1967
DOI: 10.1084/jem.125.3.393
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Studies on Rabbit Lymphocytes in Vitro

Abstract: The peripheral lymphocytes of the rabbit may be stimulated in vitro to transform into immature blast cells by sheep antisera to rabbit immunoglobulin G (IgG) and to rabbit IgG subunits (L chain, H chain, Fab piece, Fc piece), suggesting that the peripheral lymphocyte contains IgG molecules (1). Studies on lymphocytes from newborn rabbits using rabbit anti-allotype sera (2) and preincubation of lymphocytes prior to stimulation with anti-allotype (3) sera indicate that the IgG molecules present in lymphocytes ar… Show more

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Cited by 50 publications
(8 citation statements)
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“…The maximum per cent blast transformation induced by antisera of different specificities tested in this and in previous studies is given in Table IV. The high percentages induced with anti-IgG, anti-IgM, and anti-IgA sera were interpreted to indicate that all lymphocytes contained determinants of IgG and IgM; and approximately ~ of these lymphocytes contained determinants of IgA as well (5). This interpretation is inconsistant with observations that plasma cells contain only one immunoglobulin group at a time.…”
Section: Discussionmentioning
confidence: 42%
See 1 more Smart Citation
“…The maximum per cent blast transformation induced by antisera of different specificities tested in this and in previous studies is given in Table IV. The high percentages induced with anti-IgG, anti-IgM, and anti-IgA sera were interpreted to indicate that all lymphocytes contained determinants of IgG and IgM; and approximately ~ of these lymphocytes contained determinants of IgA as well (5). This interpretation is inconsistant with observations that plasma cells contain only one immunoglobulin group at a time.…”
Section: Discussionmentioning
confidence: 42%
“…(Received for publication 15 January 1968) Antisera to rabbit leukocytes (1) or to rabbit immunoglobulins (2)(3)(4)(5) may stimulate cultures of rabbit peripheral lymphocytes. Such antisera stimulate lymphocytes in vitro to synthesize RNA, DNA, and protein, to transform into immature "blast" cells, and to undergo division (2).…”
Section: (Front the Department Of Pathology University Of Pittsburghmentioning
confidence: 99%
“…Others are bound to or incorporated into the surface of cells, and we should like to think that antigen-sensitive cells precommitted to the synthesis of antibody molecules of one specificity carry samples of such molecules at their surface. Experimental evidence is available (22)(23)(24)(25) for the existence of immunoglobulin-like receptors on a majority of the lymphocytes. It is unlikely that a cell would synthesize receptor molecules of two different specificities recognizing two unrelated antigenic determinants that we have brought together in a hapten-carrier complex.…”
Section: Resultsmentioning
confidence: 99%
“…Immunofluorescence studies of plasma cells indicate that a given plasma cell contains only one immunoglobulin group at a time (9)(10)(11). In contrast, studies on the induction of lymphocyte transformation in vitro with sheep antisera specific for IgG, IgA, and IgM indicate that each lymphocyte contains the determinants of IgG and IgM at the same time; with approximately one-third of the lymphocytes containing all three major immunoglobulin groups (IgG, IgA, and IgM) at a time (12).…”
mentioning
confidence: 99%