Immunochromatographic Assay of Hepatitis B Surface Antigen Using Magnetic Nanoparticles as Signal Materials

Oh, Kyuha; Choi, Young Soo; Yoon, Ha Young; Park, Nami; Kim, Jooho; Kim, Young Keun

doi:10.1109/tmag.2014.2324613

Cited by 8 publications

(3 citation statements)

References 13 publications

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“…A comparison of several immunosensors for the quantitative detection of HBV serologic markers is summarized in Table 4. Obviously, ICA methods with various nanoparticle labels (colored, luminescent, and magnetic nanoparticles) have been widely applied in the quantitative detection of hepatitis B serological markers, with a short assay time (10~25 min), convenient operation, and good sensitivity [38][39][40][41][42]. However, clinical human serum samples have not been evaluated in the reported ICA methods [38][39][40][41], and other serologic markers, except HBsAg, were less reported.…”

Section: Discussionmentioning

confidence: 99%

“…Obviously, ICA methods with various nanoparticle labels (colored, luminescent, and magnetic nanoparticles) have been widely applied in the quantitative detection of hepatitis B serological markers, with a short assay time (10~25 min), convenient operation, and good sensitivity [38][39][40][41][42]. However, clinical human serum samples have not been evaluated in the reported ICA methods [38][39][40][41], and other serologic markers, except HBsAg, were less reported. An electrochemical method based on an AuNPs-modified glassy carbon electrode was developed to detect preS1Ag; however, it took about 80 min [43].…”

Section: Discussionmentioning

confidence: 99%

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A Rapid Immunochromatographic Method Based on a Secondary Antibody-Labelled Magnetic Nanoprobe for the Detection of Hepatitis B preS2 Surface Antigen

Cai

Yan

Zhu³

et al. 2020

Biosensors

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Hepatitis B is a globally prevalent viral infectious disease caused by the hepatitis B virus (HBV). In this study, an immunochromatographic assay (ICA) for the rapid detection of hepatitis B preS2 antigen (preS2Ag) was established. The magnetic nanoparticles (MNPs) indirectly labelled with goat anti-mouse (GAM) secondary antibody were applied as a nanoprobe for free preS2 antibody (preS2Ab) capturing and signal amplification. By employing sample pre-incubation processing as well, preS2Ag-preS2Ab was sufficiently caught by the GAM-MNPs probe in 5 min. A qualitative sensitivity of 625 ng/mL was obtained by naked-eye observation within 15–20 min. A standard curve (0–5000 ng/mL) was established, with a quantitative limit of detection (LOD) of 3.6 ng/mL, based on the stability and penetrability of the magnetic signal characteristics. The proposed method for preS2Ag was rapid (~25 min, cf. ELISA ~4 h) and had a good accuracy, which was verified using an ELISA kit (relative error < 15%). Large equipment and skilled technicians were not required. The sensitivity and specificity of the developed GAM-MNPs-ICA method were 93.3% and 90% in clinical serum samples (n = 25), respectively. A good detection consistency (84%) was observed between the developed ICA method and 2 types of commercial ELISA kits, indicating that the GAM-MNPs-ICA has a potential application in large-scale screening for and point-of-care diagnosis of hepatitis B or other infectious diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Rapid Immunochromatographic Method Based on a Secondary Antibody-Labelled Magnetic Nanoprobe for the Detection of Hepatitis B preS2 Surface Antigen

Cai

Yan

Zhu³

et al. 2020

Biosensors

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show abstract

“…Using this probe, they could enhance the analytical sensitivity of their 15 min‐LFA‐POC test down to LoD of 0.37 ng/ml. Other attempts to replace the GNPs were not as successful and LoD values of ∼1 ng/mL were achieved using red silica nanoparticles, 117 polydiacetylene vesicles 118 and iron oxide particles 119 . Using the signal amplification technique (Figure 2) by GNP‐based detecting and signal‐amplifying probes, Shen et al 115 .…”

Section: Assured‐compliant Poc Tests For the Diagnosis Of Viral Infec...mentioning

confidence: 99%

ASSURED‐compliant point‐of‐care diagnostics for the detection of human viral infections

Naseri

Ziora

Simon

et al. 2021

Reviews in Medical Virology

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Viral disease outbreaks have been always a threat to global public health making affordable, rapid and accurate diagnostics highly important tools to slow down the spread of viruses and decrease the mortality rate. Point‐of‐care (POC) diagnostics have emerged as a strong tool for the diagnosis of viral infections, especially in countries where health‐care systems are inadequate to provide proper services to all citizens. According to the World Health Organization, an ideal POC diagnostic must be Affordable, Sensitive, Specific, User‐friendly, Rapid/Robust, Equipment‐free and Deliverable (ASSURED). This review surveys carefully each ASSURED criterion and identifies where existing viral POC diagnostics fail to meet these criteria. Given the widespread concern with plastic pollution, we also propose the addition of 'disposability' to the existing ASSURED criteria and consider the letter “D” as the representative of both Deliverable and Disposable. Next, the POC tests used for the diagnosis of several common human viral infections which met all the ASSURED criteria (ASSURED‐compliant) are described in detail. Finally, the future of ASSURED‐compliant POC diagnostics, capable of generating comparable results to the viral diagnostic gold standards, is discussed.

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Influence of controlled surface functionalization of magnetic nanocomposites on the detection performance of immunochromatographic test

Zhao

Yang

et al. 2016

Sensors and Actuators B: Chemical

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Immunochromatographic Assay of Hepatitis B Surface Antigen Using Magnetic Nanoparticles as Signal Materials

Cited by 8 publications

References 13 publications

A Rapid Immunochromatographic Method Based on a Secondary Antibody-Labelled Magnetic Nanoprobe for the Detection of Hepatitis B preS2 Surface Antigen

A Rapid Immunochromatographic Method Based on a Secondary Antibody-Labelled Magnetic Nanoprobe for the Detection of Hepatitis B preS2 Surface Antigen

ASSURED‐compliant point‐of‐care diagnostics for the detection of human viral infections

Influence of controlled surface functionalization of magnetic nanocomposites on the detection performance of immunochromatographic test

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