A Rapid Immunochromatographic Method Based on a Secondary Antibody-Labelled Magnetic Nanoprobe for the Detection of Hepatitis B preS2 Surface Antigen

Cai, Yangyang; Yan, Jun; Zhu, Li; Wang, Hengliang; Lu, Ying

doi:10.3390/bios10110161

Cited by 9 publications

(10 citation statements)

References 45 publications

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“…A higher CV (>7%) was found in fish and shrimp samples, especially at low concentrations (0.1 μg/mL), implying that the food matrix could affect the detection accuracy. The obtained recoveries and CVs by the developed TS-DTL were comparable to the ICTS methods reported previously [ 13 , 29 ]. In addition, the measurement results of three types of real samples by TS-DTL were in agreement with the data obtained by the well-accepted HPLC method, showing that the developed tricolour TS-DTLS could accurately and quantitatively detect TMS and MG in aquatic products and related aquaculture water.…”

Section: Resultssupporting

confidence: 84%

“…In recent years, immunochromatographic test strip (ICTS) technology based on specific reactions between monoclonal antibodies (mAbs) and antigens has been widely applied in the fields of food safety [ 12 ] and disease diagnosis [ 13 ] due to its advantages of a simple operation, low cost and fast on-site detection. The common ICTS employs coloured or luminescent nanoparticles as detection probes, such as Au nanoparticles (AuNPs) [ 14 ], silica nanoparticles [ 15 ] and quantum dots [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

Xü

Liu

et al. 2022

Biosensors

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In this research, we designed a rapid tricolour immunochromatographic test strip with double test lines (TS-DTL) and two-colour AuNP probes, which realised the simultaneous detection of tricaine mesylate (TMS) and malachite green (MG). Through a distinct tricolour system (red T1 line, blue T2 line and purple C line), a visual identification of TMS (0.2 μg/mL) and MG (0.5 μg/mL) was quickly achieved on site, which improved the accuracy of naked eye observations. The LODs of TMS in aquaculture water, fish and shrimp were 11.0, 29.6 and 61.4 ng/mL, respectively. MG LODs were 47.0 ng/mL (aquaculture water), 82.8 ng/mL (fish) and 152.4 ng/mL (shrimp). The LOD of MG was close to the similar TS methods. However, visual detection of TMS could meet the requirements of the residue limit (1 μg/mL) of TMS in the USA, and the quantitative detection of TMS was over 16 times lower than the USA standard. The developed platform was rapid (~20 min, HPLC~3 h) and accurate, which was verified using a traditional HPLC method. The recovery rates ranged from 82.2% to 108.6% in three types of real samples, indicating a potential application in on-site fast screening or multiple detection for TMS and MG residues in aquatic products.

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Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

Xü

Liu

et al. 2022

Biosensors

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“…This is mainly attributed to the application of colloidal gold as a reporter. In recent years, researchers have tried to develop various nano-beacon materials to replace colloidal gold, including fluorescent microspheres, up-conversion luminescence materials, , quantum dots, , and magnetic particles, , and so on. Among them, surface-enhanced Raman scattering (SERS) nanotags can provide strong fingerprint signals and have been combined with the LFIA platform to develop ultra-sensitive indicators for trace biological substances. − …”

Section: Introductionmentioning

confidence: 99%

Ag Nanoparticles with Ultrathin Au Shell-Based Lateral Flow Immunoassay for Colorimetric and SERS Dual-Mode Detection of SARS-CoV-2 IgG

et al. 2022

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Immunoglobulin detection is essential for diagnosing progression of SARS-CoV-2 infection, for which SARS-CoV-2 IgG is one of the most important indexes. In this paper, Ag nanoparticles with ultrathin Au shells (∼2 nm) embedded with 4-mercaptobenzoic acid (MBA) (Ag MBA @Au) were manufactured via a ligand-assisted epitaxial growth method and integrated into lateral flow immunoassay (LFIA) for colorimetric and SERS dual-mode detection of SARS-CoV-2 IgG. Ag MBA @Au possessed not only the surface chemistry advantages of Au but also the superior optical characteristics of Ag. Moreover, the nanogap between the Ag core and the Au shell also greatly enhanced the Raman signal. After being modified with anti-human antibodies, Ag MBA @Au recognized and combined with SARS-CoV-2 IgG, which was captured by the SARS-CoV-2 spike protein on the T line. Qualitative analysis was achieved by visually observing the color of the T line, and quantitative analysis was conducted by measuring the SERS signal with a sensitivity four orders of magnitude higher (detection limit: 0.22 pg/mL). The intra-assay and inter-assay variation coefficients were 7.7 and 10.3%, respectively, and other proteins at concentrations of 10 to 20 times higher than those of SARS-CoV-2 IgG could hardly produce distinguishable signals, confirming good reproducibility and specificity. Finally, this method was used to detect 107 clinical serum samples. The results agreed well with those obtained from enzyme-linked immunosorbent assay kits and were significantly better than those of the colloidal gold test strips. Therefore, this dual-mode LFIA has great potential in clinical practical applications and can be used to screen and trace the early immune response of SARS-CoV-2.

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“…Besides, the single detection mode based on colorimetric results is susceptible to interference from biological environment with high background, particularly serum and plasma. Recent studies have applied colorimetric/fluorescence [ 19 ], colorimetric/luminescence [ 20 ] and colorimetric/surface-enhanced Raman spectroscopy (SERS) [ 21 , 22 ] dual-mode analytical models to LFIA platform [ 4 ], based on colloidal gold [ 17 ], carbon nanoparticles [ 23 ], fluorescent nanoparticles [ 24 – 27 ], SERS-active nanomaterials [ 28 , 29 ], and magnetic nanoparticles [ 30 , 31 ] as the LFIA reporters.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric and Raman dual-mode lateral flow immunoassay detection of SARS-CoV-2 N protein antibody based on Ag nanoparticles with ultrathin Au shell assembled onto Fe3O4 nanoparticles

Liang

Zhao

et al. 2022

Anal Bioanal Chem

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Serological antibody tests are useful complements of nuclei acid detection for SARS-CoV-2 diagnosis, which can significantly improve diagnostic accuracy. However, antibody detection in serum or plasma remains challenging to do with high sensitivity. In this study, Ag nanoparticles with ultra-thin Au shells embedded with 4-mercaptobenzoic acid (MBA) (Ag MBA @Au) were manufactured and then assembled onto Fe 3 O 4 surface by electrostatic interaction to construct the Fe 3 O 4 -Ag MBA @Au nanoparticles (NPs) with magnetic-Raman-colorimetric properties. Based on the composite nanoparticles, a colorimetric and Raman dual-mode lateral flow immunoassay (LFIA) for ultrasensitive identification of SARS-CoV-2 nucleocapsid (N) protein antibody was constructed. The magnetic nanoparticles (Fe 3 O 4 NPs) were acted as the core and coated a layer of Ag MBA @Au particles on the surface by electrostatic interaction to prepare Fe 3 O 4 -Ag MBA @Au NPs, which can amplify the SERS signal due to multiple Ag MBA @Au particles concentrated on a single magnetic nanoparticle. Moreover, the Fe 3 O 4 -Ag MBA @Au NPs facilitated pre-purifying sample using magnetic separation, and complex matrix interference would be greatly decreased in the detection. The Fe 3 O 4 -Ag MBA @Au NPs modified with N protein recognized and bound with N protein antibodies, which were trapped on the T-line, forming color band for observing detection. Under optimal conditions, the N protein antibodies could be qualitatively detected in colorimetric mode with the visual limit of 10 −8 mg/mL and quantitatively detected by SERS signals between 10 −6 and 10 −10 mg /mL with 0.08 pg/mL detection limit. The coefficients variations (CV) of intra-assay was 8.0%, whereas of inter-assay was 11.7%, confirming of good reproducibility. Finally, this approach was able to discriminate between positive, negative, and weakly positive samples when detecting 107 clinical serum samples. The process enables highly sensitive quantitative assays that are valuable for evaluating disease processes and guiding treatment. Graphical Abstract Colorimetric and Raman dual-mode LFIA detection of SARS-CoV-2 N protein antibody based on Fe 3 O 4 -Ag MBA @Au nanoparticles Supplementary Information The online version contains supplementary material available at 10.1007/s00216-022-04437-1.

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A Rapid Immunochromatographic Method Based on a Secondary Antibody-Labelled Magnetic Nanoprobe for the Detection of Hepatitis B preS2 Surface Antigen

Cited by 9 publications

References 45 publications

A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

A Rapid Tricolour Immunochromatographic Assay for Simultaneous Detection of Tricaine and Malachite Green

Ag Nanoparticles with Ultrathin Au Shell-Based Lateral Flow Immunoassay for Colorimetric and SERS Dual-Mode Detection of SARS-CoV-2 IgG

Colorimetric and Raman dual-mode lateral flow immunoassay detection of SARS-CoV-2 N protein antibody based on Ag nanoparticles with ultrathin Au shell assembled onto Fe3O4 nanoparticles

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