Immunochromatographic assay for melamine based on luminescent quantum dot beads as signaling probes

Abstract

An immunochromatographic assay using quantum dot beads as a label was established for melamine detection in milk with fast and effective pretreatment.

Modification of a nitrocellulose membrane with nanofibers for sensitivity enhancement in lateral flow test strips

“…Research in this eld has developed various methods for improving the sensitivity of LFA, including sample pretreatment, [14][15][16][17] modication of labeled antibody nanoparticles, [18][19][20][21][22] nanozyme signal enhancement, [23][24][25][26] and targeted xation of antibodies. 27 Besides, many researches have been done focusing on modication of the key component of the test strip, i.e., the nitrocellulose (NC) membrane by introducing functional groups onto the surface or increase the specic surface area to improve the sensitivity of the test strip.…”

Detection of melamine based on the suppressed anodic response of uric acid by a Au-Ag nanoparticles modified glassy carbon electrode

“…Several instrumental methods have been developed for the detection and estimation of Mel, such as colorimetric assay, enzyme-linked immunosorbent assay, fluorescence spectroscopy, surface-enhanced Raman spectroscopy, capillary electrophoresis–mass spectrometry, and liquid chromatography–mass spectrometry [ 2 – 9 ]. Although these methods produce accurate and precise results, they are expensive to employ, time consuming, and require tedious sample preparation [ 10 – 21 ]. Furthermore, they cannot be employed for rapid on-site analysis [ 22 ].…”

Sensitive Immunochromatographic Assay Using Highly Luminescent Quantum Dot Nanobeads as Tracer for the Detection of Cyproheptadine Hydrochloride in Animal-Derived Food

“…However, it does not provide advanced sensitivity comparisons with conventional ELISA. A alternative reporter, quantum dot nanobeads (QBs) could have been utilized to improve the detection sensitivity of ICA, because of their high quantum yield, broad UV excitation with narrow fluorescent emission spectra, large molar extinction coefficient, stronger fluorescence intensity and high photostability (Duan et al, 2015 , 2019 ; Xiao et al, 2018 ; Chen et al, 2020 ). Wang et al used QBs as luminescent amplification probes for ultrasensitive detection of tebuconazole in agricultural product and achieved a LOD of 0.02 ng/mL, which was more sensitive than other reports (Wang Y. et al, 2019 ).…”