Immunochemical studies of organ and tumor lipids.II. Organ and species specificity of the lipid antigens of the rat lymphosarcoma

Immobilization of α‐chymotrypsin on sucrose stearate‐palmitate containing liposomes

“…The increase in SSP molar concentration (25-40 mol%) leads to aggregation of the liposome preparation even in the presence of 10 mololo of dicetylphosphate as the negatively charged liposome component. SSP liposomes retained more than 70% of the initial amount of the entrapped calcein for a 3-day period which is comparable to data obtained with SSP-free liposomes (table 1) [16] we found that 15% of SSP was oxidized when liposomes were incubated overnight at pH 5.6 in the presence of 10m2 M NaI04.2HzO.…”

Identification of the major glycolipid from Mycoplasma species strain J as 3,4,6-triacyl-β-D-glucopyranose

“…Fatty acid esters were determined by hydroxamate formation (Rapport and Alonzo, 1955); reducing sugar by the Park-Johnson (1949) method; hexose by the anthrone reaction; glucose and galactose by their respective oxidases (Worthington Biochemicals Corp., Freehold, N. J.…”

Distribution of Alkylglycerone-Phosphate Synthase in Subcellular Fractions of Rat Liver

“…The essential reactions of the biosynthesis of O-alkyl-glycerolipids have largely been elucidated by Snyder et al 141 and Hajra 151 , and are known as dihydroxyacetone phosphatepathway. They comprise (i) the acylation of glycerone phosphate (also known as dihydroxyacetone phosphate which is the main precursor of ether lipids) by a fatty acid 16 ' 71 , (ii) the subsequent substitution of a long-chain alcohol for even this acyl group i8] and (iii) the reduction of the alkyl derivative thus obtained by an NADPHdependent reductase giving rise to the ether analog of a lysophosphatidic acid 141 . In rat and guinea pig liver the acyltransferase catalysing the acylation of glycerone phosphate is mostly localized in peroxisomes whereas the reductase was shown to be enriched in both peroxisomes and microsomes* 3 ' 9 ' 101 .…”