Immunochemical determination of human apolipoprotein A-I by laser nephelometry

Moderate alcohol consumption is associated with lower cardiovascular mortality. The effect of moderate alcohol intake during 5 weeks on lipoproteins, especially on the high density lipoprotein (HDL) cholesterol total (of which the levels are inversely predictive of coronary heart disease) and apoproteins A, A1 and B, was studied in 7 normal men. HDL cholesterol total appreciated by the heparin manganese precipitation method and phosphortungstate magnesium method increased (p < 0.01) during alcohol consumption. The composition of HDL was modified by alcohol consumption: increase of the esterified/total cholesterol ratio (p < 0.05) and phospholipids (p < 0.05) without significant modification of triglycerides. Low density lipoprotein and very low density lipoprotein did not vary significantly. Apoprotein A1 increased during alcohol consumption (p < 0.05) with a transitory increase of apoprotein A. There was no significant modification of apoprotein B.

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“…Apoproteins A, A1 and B were assayed by the immunonephelometric technique [17,18]. The usual quality control procedures were followed.…”

mentioning

confidence: 99%

High Density Lipoprotein Cholesterol and Apoprotein A1 in Healthy Volunteers during Long-Term Moderate Alcohol Intake

Couzigou¹,

Fleury

Crockett

et al. 1984

Ann Nutr Metab

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“…Plasma was then separated and sodium azide (0.2 g/l) added. Plasma apo B (Sievet-Desrumeaux et al 1979) and apo A1 (Sievet-Desrumeaux et al 1980) were measured by immunonephelometry with Hyland Travenol reagents. The lipoprotein profiles were obtained by means of density-gradient ultracentrifugation according to A. Mougin-Schutz (personal communication).…”

Section: Lipid Analysismentioning

confidence: 99%

Effects of a low-energy diet associated with egg supplementation on plasma cholesterol and lipoprotein levels in normal subjects: results of a cross-over study

et al. 1986

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1. The influence of a low-energy diet when associated with high-cholesterol intake was investigated in seventeen normal men during an 8-week cross-over study. The subjects were given a daily supplement of two whole eggs and two egg yolks (approximately 1 g cholesterol) either with their usual diet for 4 weeks or with a low-energy diet for 4 weeks. Each subject took part randomly in both dietary periods.2. During the first part of the study, no changes occurred in the plasma cholesterol of the subjects with egg supplementation of the usual diet. 3.In contrast, the low-energy diet and associated weight loss markedly decreased the tolerance to highcholesterol intake resulting in increased plasma cholesterol. The mean rise was 22.7% but with wide individual variations in the response. This was almost completely normalized when the subjects returned to their usual energy intake indicating the involvement of weight reduction in the increase observed.4. Changes in low-density-lipoprotein (LDL) cholesterol were parallel to those of total plasma cholesterol with an increase following the low-energy diet and normalization after body-weight recovery.5. The opposite effect was shown with the low-energy diet after previous adaptation to the consumption of four eggs per day. This dietary regimen resulted in a decrease in plasma cholesterol although it was not significant. Moreover, the lipoprotein profile was improved with a decrease in very-low-density-lipoprotein (VLDL) cholesterol and an increase in high-density-lipoprotein (HDL) cholesterol.6. High-cholesterol intake induced significant changes in lipoprotein composition whatever the energy ration. LDL and HDL were enriched in cholesterol esters as early as the 1st month of egg supplementation of the diet.7. Taken together, the results emphasize the possible adverse effect of slimming diets when associated with high-cholesterol intake. The existence of ' high-responders' to these dietary conditions calls for special attention to be paid to the cholesterol content of restricted diets.

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Plasma lipid lowering effects of wheat germ in hypercholesterolemic subjects

Cara

Borel

Armand

et al. 1991

Plant Food Hum Nutr

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The present study was performed to investigate the possible effects of wheat germ supplementation on lipid metabolism in humans. Ten free-living adult subjects participated in the study. None was obese or diabetic. They all presented an hypercholesterolemia (from 6.58 to 9.50 mM), associated in 6 over 10 cases to an hypertriglyceridemia (from 1.70 to 5.00 mM). The subjects were studied in three consecutive periods, during which they first were on their usual diet (first week), they then ingested a daily supplement of 30 g wheat germ (4 weeks) and then they returned to their usual basal diet (4 weeks follow-up). Dietary records were obtained for 7 and 3 consecutive days before and during wheat germ supplementation, respectively. Fasting blood samples were taken at the end of each period. After 4 weeks of wheat germ intake, glycemia did not change while total plasma cholesterol significantly decreased (paired Student's t test, p less than or equal to 0.05) from 7.80 to 7.15 mM. LDL and HDL cholesterol values did not show marked changes, but VLDL cholesterol significantly dropped by 40.6%. Thus, the plasma/HDL total cholesterol ratio was significantly lower. Apoprotein B and A1 decreased. In the hypertriglyceridemic subjects, this was accompanied by a significant reduction of plasma triglycerides (1.64 vs. 2.68 mM) and a marked drop of VLDL triglycerides (-51%). Taken as a whole, the present results obtained in humans are very close to those previously obtained in the rat and point out that wheat germ may play a beneficial role in the dietary management of hyperlipidemia.

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Immunochemical determination of human apolipoprotein A-I by laser nephelometry

Cited by 24 publications

References 19 publications

High Density Lipoprotein Cholesterol and Apoprotein A1 in Healthy Volunteers during Long-Term Moderate Alcohol Intake

High Density Lipoprotein Cholesterol and Apoprotein A1 in Healthy Volunteers during Long-Term Moderate Alcohol Intake

Effects of a low-energy diet associated with egg supplementation on plasma cholesterol and lipoprotein levels in normal subjects: results of a cross-over study

Plasma lipid lowering effects of wheat germ in hypercholesterolemic subjects

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