Immunochemical detection ofSalmonellagroup B, D and E using an optical surface plasmon resonance biosensor

Abstract

A surface plasmon resonance biosensor (Biacore) was used to detect Salmonella through antibodies reacting with Salmonella group A, B, D and E (Kauffmann^White typing). In the assay designed, anti-Salmonella antibodies immobilized to the biosensor surface were allowed to bind injected bacteria followed by a pulse with soluble anti-Salmonella immunoglobulins to intensify the signal. No significant interference was found for (mixtures of) 30 non-Salmonella serovars at 10 9 CFU ml 31 . A total of 53 Salmonella serovars were successfully detected at 1U10 7 CFU ml 31 , except those of groups C, G, L and P, as expected. The cut-off point was determined with an equicellular mixture of Salmonella enteritidis and Salmonella typhimurium at a final amount of 1.7U10 3 CFU per test portion. Although further work is needed to cover the detection of all relevant Salmonella serovars in food-producing animals and food products, this work demonstrates the merits of this alternative biosensor approach in terms of automation, sensitivity, specificity, simple handling and limited hands-on time. ß

Development of a Simultaneous Detection Method for Foodborne Pathogens Using Surface Plasmon Resonance Biosensors

“…Many researchers have applied SPR biosensors for the detection of O157 (Meeusen et al, 2005;Subramanian et al, 2006;Taylor et al, 2005), SE (Bhunia et al, 2004;Bokken et al, 2003), and LM (Hearty et al, 2006;Leonard et al, 2004). The lower detection limit was reported to be 10 5 to 10 7 CFU/mL for O157, 10 6 to 10 7 CFU/mL for SE, and 10 5 to 10 7 CFU/mL for LM, respectively.…”

Enzyme-based electrochemical biosensors for food safety: a review

“…48 Many researchers use commercially available SPR biosensor for the identification of food-borne pathogens. BIACORE 3000 was used for the detection of Listeria monocytogenes 49 and Salmonella, 50 and Spreeta TM was used for E. coli O157:H7. 51,52 Nonspecific antigen Antigen Antibody Substrate Figure 6 Antigen-antibody interaction taking place at the surface of the transducer.…”

Biosensors and their Applications in Food Safety: A Review

“…can survive outside of their natural habitat, e.g., in water and food products (White et al, 2002). Salmonella detection by SPR-based assays with antibodies as the recognition element has been described in many studies (Bokken et al, 2003;Oh et al, 2004;Mazumdar et al, 2007;Dudak and Boyaci, 2009;Mazumdar et al, 2010;Singh et al, 2015;Vaisocherová-Lísalová et al, 2016).…”