Immunochemical characterization and quantitation of lipoprotein (a) in baboons

Rainwater, David L.; Manis, Georgios

doi:10.1016/0021-9150(88)90159-1

Cited by 37 publications

(2 citation statements)

References 21 publications

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“…As a first step in the identification of these nucleotides, we compared the chimpanzee apo(a) promoter sequence to the corresponding human and baboon sequences. Lp(a) concentrations in baboon are not sig- nificantly different from those observed in Caucasians (26). Therefore, assuming that the baboon and human apo(a) promoters possess similar transcriptional activities, we hypothesized that the identification of base changes specific to the chimpanzee apo(a) sequence could be relevant to the increased apo(a) promoter activity observed in this species.…”

Section: Resultsmentioning

confidence: 99%

Functional Analysis of the Chimpanzee and Humanapo(a) Promoter Sequences

Huby

Dachet

Lawn

et al. 2001

Journal of Biological Chemistry

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Section: Resultsmentioning

confidence: 99%

Functional Analysis of the Chimpanzee and Humanapo(a) Promoter Sequences

Huby

Dachet

Lawn

et al. 2001

Journal of Biological Chemistry

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“…Another ELISA technique was developed for the measurement of Lp(a) concentrations in baboons. The assay has been documented to quantify Lp(a) concentrations from l to 9 ng [61].…”

Section: Elisamentioning

confidence: 99%

The ins and outs of lipoprotein(a) assay methods

Heydari,

Rezayi,

Ruscica

et al. 2024

Arch Med Sci Atheroscler Dis

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Pathophysiological, epidemiological and genetic studies convincingly showed lipoprotein(a) (Lp(a)) to be a causal mediator of atherosclerotic cardiovascular disease (ASCVD). This happens through a myriad of mechanisms including activation of innate immune cells, endothelial cells as well as platelets. Although these certainties whether or not Lp(a) is ready for prime-time clinical use remain debated. Thus, remit of the present review is to provide an overview of different methods that have been employed for the measurement of Lp(a). The methods include dynamic light scattering, multi-angle light scattering analysis, near-field imaging, sedimentation, gel filtration, and electron microscopy. The development of multiple Lp(a) detection methods is vital for improved prediction of ASCVD risk.

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Automated latex nephelometric immunoassay for the measurement of serum lipoprotein (a)

Borque

Rus

Cura

et al. 1993

Clinical Laboratory Analysis

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A sensitive immunoassay based on latex particle agglutination has been developed for measuring lipoprotein Lp(a) concentrations in serum or plasma. Carboxylated latex particles (diameter 240 nm) covalently coated with F(ab')2 fragments of anti-lipoprotein Lp(a) antibodies are incubated with diluted sample (400-fold) for 12 min at room temperature, with the resulting agglutination quantified by measuring the change of light-scatter produced. The assay has been automated on the Behring nephelometer analyzer with a sampling rate of 150 samples/hour. This assay generates a standard curve in the range of 27 to 1750 mg/L, showing inter-assay precision of less than 8%. There were no interferences from plasminogen, bilirubin, Intralipid, haemoglobin, rheumatoid factor, and apolipoprotein B. No significant differences were observed when fresh and frozen samples were compared. Sample pretreatment with "Lipoclean" clearing agent and sample lyophilization decreased the agglutinating reaction. In two separate studies using 77 and 112 patient sera the Lp(a) values, determined by the latex nephelometric method, the Terumo Macra Lp(a) ELISA test, and the Pharmacia Apo(a) radioimmunoassay method, gave correlation coefficients of 0.948 and 0.974, respectively. Physiological lipoprotein (a) values were determined in a blood donor group, with the distribution of serum Lp(a) highly skewed, with a mean (SD) and median values of 213(236) mg/L and 116 mg/L, respectively. Concentrations of Lp(a) were found to be age- and sex-independent. This latex nephelometric procedure is a convenient method and an interesting alternative to other immunoassays for routine measurement of human lipoprotein (a).

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Immunochemical characterization and quantitation of lipoprotein (a) in baboons

Cited by 37 publications

References 21 publications

Functional Analysis of the Chimpanzee and Humanapo(a) Promoter Sequences

Functional Analysis of the Chimpanzee and Humanapo(a) Promoter Sequences

The ins and outs of lipoprotein(a) assay methods

Automated latex nephelometric immunoassay for the measurement of serum lipoprotein (a)

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