Immunoblot evaluation of the 100 and 130 kDa antigens in camel hydatid cyst fluid for the serodiagnosis of human cystic echinococcosis in Libya

Abstract

Two high molecular weight antigens with molecular masses approximately 100 kDa and 130 kDa were identified by immunoblotting camel hydatid cyst fluid, with 94% sensitivity in sera from surgically confirmed Libyan cystic echinococcosis cases. 40% of sera from surgically confirmed alveolar echinococcosis cases cross-reacted with the 100 and 130 kDa antigens, as did 5.3% of sera from human Taenia solium cysticercosis patients. No cross-reaction occurred with sera from human schistosomiasis mansoni or onchocerciasis patients. In addition, all sera from patients with non-hydatid space-occupying lesions (i.e. simple liver cysts, kidney cysts, lung tuberculosis, pulmonary carcinoma, pulmonary empyema, and lung abscess) were seronegative against the same antigens, as were control serum samples from healthy individuals. The 100 and 130 kDa antigens were strongly recognized by sera from cystic echinococcosis patients when camel or horse hydatid cyst fluid was used in immunoblotting but were only weakly recognized if sheep or human hydatid cyst fluid was used. Camel hydatid cyst fluid could be an important source of diagnostic antigens for human cystic echinococcosis in the Middle East endemic region.

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18).

“…The sensitivity (92%) for the 8-kD subunit obtained in our study appears to be similar to that (94%) for the 8-kD subunit reported by Shambesh and others, 11 as well as that (90%) for the 12-kD subunit described by Leggatt and others, 10 but higher than that (80%) for the 12-kD subunit reported by Ioppolo and others. 31 The cross-reactions for the 8-kD subunit with AE sera (80%) demonstrated in our study is higher than those reported in other two studies (both 40%).…”

“…31 The cross-reactions for the 8-kD subunit with AE sera (80%) demonstrated in our study is higher than those reported in other two studies (both 40%). 10,11 Based on these data, it seems reasonable to conclude that the smallest subunit of antigen B (8/12-kD) is not species-specific for E. granulosus but is shared with E. multilocularis. This will limit its usefulness for serologic differentiation of CE from AE.…”

“…Currently, it has been generally accepted that detection of antigen B, particularly its smallest subunit of 8/12 kD, offers greater specificity than detection of antigen 5, but neither antigen B nor antigen 5 are species-specific for E. granulosus. [8][9][10][11] For the serodiagnosis of AE, an ELISA using an affinity-purified Em2 antigen from E. multilocularis metacestodes has been shown to be a reliable serologic test. 12 Its diagnostic value has been improved by the combination use of the native Em2 antigen and a recombinant antigen (II/3-10) in an ELISA known as the Em2 plus -ELISA, which is now commercially available.…”

Shared and non-shared antigens from three different extracts of the metacestode of Echinococcus granulosus

“…Some of the products identified in HCF by the pool of human sera in the present study may correspond to those previously described by other authors using the same antigenic extract. This may be the case of the protein of 34 kDa (recently identified by Poretti et al 1999), and the protein of 110 kDa, probably related with the component of 100 kDa described by Shambesh et al (1995), the protein of 110-120 kDa (Shapiro et al 1992), and the protein of 116 kDa (Kanwar et al 1992).…”

Comparative evaluation of Western Blotting in hepatic and pulmonary cystic echinococcosis

“…According to the ROC curve analysis, the band of 120-124 kDa is observed as the most important band for differentiation. There aren't enough studies about high molecular masses in CE patients (Sbihi et al, 1997;Kanwar et al, 1992;Shambesh et al, 1995). In a previous study, we frequently found a band of 124 kDa in patients with pulmonary CE (Akisu et al, 2005).…”