Shared and non-shared antigens from three different extracts of the metacestode of Echinococcus granulosus

Carmena, David; Martı́nez, Jorge; Benito, Aitziber; Guisantes, Jorge A.

doi:10.1590/s0074-02762005000800007

Cited by 8 publications

(8 citation statements)

References 27 publications

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“…Thus far, the use of these antigens is predominantly restricted to scientific applications, and neither is available for general use (5). The number of reports on the use of protoscolex as an antigen for detecting antibodies in human hydatidosis is limited (16)(17)(18)(19). Chemale et al (20) described the analysis of E. granulosus metacestode protein extract by 2-DE and the identification of prominent proteins by peptide mass fingerprinting (PMF).…”

Section: Discussionmentioning

confidence: 99%

IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

Moghadam

Ghaffarifar

Khalilpour

et al. 2013

Clin Vaccine Immunol

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Hydatidosis is a public health problem in many parts of the world, and improvement in diagnosis of the disease is still being pursued. Protoscoleces of Echinococcus granulosus were isolated from hydatid cysts collected from naturally infected sheep slaughtered in abattoirs in Iran. Sonicated extract of protoscolex was subjected to two-dimensional gel electrophoresis and Western blot analysis. Primary antibodies were from serum samples from 130 hydatidosis patients, 38 individuals infected with other parasitic infections, and 30 healthy people, whereas peroxidase (HRP)-conjugated anti-human IgG and IgG4 were used as secondary antibodies. The recombinant form of the identified protein was produced and tested for its sensitivity and specificity for the detection of human hydatidosis. An antigenic band of ϳ60 kDa was found to be sensitive (82%) and specific (100%) for the detection of hydatidosis when probed with anti-human IgG4-HRP, while the sensitivity and specificity were 33 and 100%, respectively, with anti-human IgG-HRP. By mass spectrometry, the band was identified as protoscolex tegument paramyosin. The sensitivity and specificity of full-length paramyosin-recombinant protein in IgG4 blots were found to be 86 and 98%, respectively. In conclusion, IgG4 detection of Echinococcus granulosus paramyosin was found to be useful for the diagnosis of human hydatidosis.

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Section: Discussionmentioning

confidence: 99%

IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

Moghadam

Ghaffarifar

Khalilpour

et al. 2013

Clin Vaccine Immunol

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“…In E. granulosus protoscolex ESP, two new E. granulosus antigens of 89 kDa and 74 kDa have been proposed as potential diagnostic molecules for the immunodiagnosis of human CE [65]. Subsequent cross‐reactivity studies by immunoblot and ELISA inhibition demonstrated that E. granulosus protoscolex ESPs share a high proportion of antigenic components with protoscolex somatic extracts and, to a lesser extent, with hydatid fluid [66].…”

Section: Antigens For the Immunodiagnosis Of Aementioning

confidence: 99%

The immunodiagnosis of Echinococcus multilocularis infection

Carmena

Benito

Eraso

2007

Clinical Microbiology and Infection

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Alveolar echinococcosis (AE) is a severe zoonotic disease caused by the metacestode stage of Echinococcus multilocularis. The infection can have fatal consequences in humans if treatment is not provided, so early diagnosis is fundamental for initiating treatment and reducing morbidity and mortality. In addition, detection of the parasite in the definitive host plays a central role in epidemiological studies and surveillance programmes for control of AE. This review presents an overview of the present situation regarding the immunodiagnosis of E. multilocularis infection. Special attention is given to the description of the native, partially purified and recombinant antigens available currently for immunodiagnostic purposes. Recent advances in the primary serodiagnosis and follow-up of AE patients are highlighted, including the detection of specific cytokine profiles. Progress in the immunodiagnosis of intestinal E. multilocularis infection in definitive hosts, particularly the detection of excretory-secretory and integument products of the worm in faeces (copro-antigens) by ELISA, is also discussed.

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“…Also, variation in mean OD values within the infected groups may be related to different sensitivity of each Ag as well as the specific level Abs titer in their sera. Few studies used soluble protoscolices antigen in diagnosing human echinococcosis (Carmena et al, 2004), but many proteins of E. granulosus as hydatid fluid & protoscolices were immunogenic and used to diagnose specific Abs in dogs and intermediate host as sheep (Carmena et al, 2005). Fotoohi et al (2013) reported the suitability of HC Ag of sheep origin to diagnose infection in patients.…”

Section: Discussionmentioning

confidence: 99%

Protoscolices Antigens From Hydatid Cyst of Human and Animal Origin for Diagnosis of Cystic Echinococcosis

Ramadan

Khalifa

Kamel

et al. 2020

Journal of the Egyptian Society of Parasitology

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Shared and non-shared antigens from three different extracts of the metacestode of Echinococcus granulosus

Cited by 8 publications

References 27 publications

IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

The immunodiagnosis of Echinococcus multilocularis infection

Protoscolices Antigens From Hydatid Cyst of Human and Animal Origin for Diagnosis of Cystic Echinococcosis

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