Comparative evaluation of Western Blotting in hepatic and pulmonary cystic echinococcosis

Abstract

Summary :Many serological tests are widely used in the diagnosis of cystic echinococcosis (CE), caused by the larval stages of Echinococcus granulosus. The present study was carried for differentiation between hepatic and pulmonary cystic echinococcosis by Western Blotting (WB). A total of 121 sera from patients with hepatic CE (37), pulmonary CE (31) and controls (53; consisting of six healthy, seven Hymenolepis nana infection, 20 hepatic and 20 pulmonary diseases other than CE) were examined. In all of the CE patients, E. granulosus infection was confirmed by surgical intervention. Sera were previously tested using IHA and ELISA to detect the E. granulosus specific antibodies. Sera from hepatic cases of CE reacted with 16 polypeptides of 6-116 kDa and sera from pulmonary cases of CE reacted with 14 polypeptides of 4-130 kDa by Western Blotting. The WB test enabled the detection of antibodies in the hepatic CE samples for proteins of 24, 32-34, 44-46 and 52-54 kDa in molecular weight in 78.4 %, 75.7 %, 78.4 % and 89.2 % of the patients, respectively. In the pulmonary CE samples sera WB test enabled the detection of antibodies 24, 44-46, 100, 110, 116 and 120-124 kDa in molecular weight in 81.3 %, 75.0 %, 87.5 %, 71.9 %, 84.4 % and 65.6 % of the patients, respectively. We indicated that the antigenic components of high molecular weight can be good candidates for differentiation of hepatic CE from pulmonary CE. de 24,[32][33][34][44][45][46]4 %, 75,7 %, 78,4 % et 89,2 % des patients, respectivement, et, en cas de KH pulmonaire, de protéines de 24,[44][45][46] 100, 110,[116][117][118][119][120][121][122][123][124]3 %, 75,0 %, 87,5 %, 71,9 %, 84,4 % et 65,6 Résumé

Serological Diagnosis and Follow-Up of Human Cystic Echinococcosis: A New Hope for the Future?

“…Se reported in these studies varied from 64.8% to 100%. Reasons for false negative results depend on several factors comprising cyst location other than the liver [ 12 , 13 ], early (CE1) and inactive (CE4 and CE5) cyst stages [ 14 – 16 ], serum collection before treatment [ 15 , 17 ], single and small cysts [ 17 ], and HF antigenic source variability [ 18 ]. Additionally, E. granulosus complex comprises several genotypes that potentially express different antigenic sets.…”

Evaluation of the seropositivity of patients with cystic echinococcosis in Konya, Turkey

“…Conducted in our country, CH is higher in women than in men. [3,16,17] According to the study of Hakverdi et al [18] according to their study, the rate of CH was found to be 53.7% in women and 46.3% in men. In a study from the Central Anatolia Region, 56% of the patients diagnosed with CH were reported as female and 44% as male.…”

Turkish HPB Surgery Association consensus report on hepatic cystic Echinococcosis (HCE)

“…Another recommendation for performance is to use the WB (Western Blot) test as a secondary confirmatory test due to its high specificity of up to 100% (2,6,(21)(22)(23)(24). It is possible to find different results for each technique in the literature due to the aforementioned factors, especially the type of antigen used in the test (18,(25)(26)(27)(28)(29).…”

“…The most commonly used antigen source for this purpose in tests is hydatid cyst fluid (HCS). Overall, HCS provides higher sensitivity (80-99% sensitivity and 60-97% specificity), while tests in which the purified main antigenic structures of Antigen 5 (Ag5) and Antigen B (AgB) or their smaller subunits or their recombinant forms and synthetic peptides are used as antigens provide higher specificity (33-93% sensitivity and 80-100% specificity) (27)(28)(29)(30). A double-blind, randomized study on this subject compared the diagnostic performances of HCS, natural AgB, two recombinant AgB subunits, an AgB-derived synthetic peptide, and recombinant cytosolic malate dehydrogenase (EgM-DH) in the ELISA method (31).…”