Immunoassay based on monoclonal antibody for aflatoxin detection in poultry feed

Rossi, Carolina Nachi; Takabayashi, Cássia Reika; Ono, Mário Augusto; Saito, Gervásio Hitoshi; Itano, Eiko Nakagawa; Kawamura, Osamu; Hirooka, Elisa Yoko; Ono, Elisabete Yurie Sataque

doi:10.1016/j.foodchem.2011.12.067

Cited by 62 publications

(41 citation statements)

References 21 publications

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“…High-performance liquid chromatography (HPLC) (Hu, Zheng, Zhang, & He, 2006), thin layer chromatography (TLC) (Lin, Zhang, Wang, Wang, & Chen, 1998), enzyme-linked immunosorbent assay (ELISA) (Rossi et al, 2012) and fluorescence methods have been used for the detection of aflatoxins. However, most of these techniques require well equipped laboratories, trained personnel, harmful solvents and are time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oil

Zhang

et al. 2015

Food Chemistry

118

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Section: Introductionmentioning

confidence: 99%

Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oil

Zhang

et al. 2015

Food Chemistry

118

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“…Of the 7 positive samples of competitive ELISA tested, only 3 samples showed positive HPLC. This is probably due to the influence of protein content, pigment, and amino acids that estimated could increase toxin levels in testing by competitive ELISA methods (Rossi et al, 2012). Comparison of the ELISA method, ability to detect aflatoxins to be rugged, sensitive, accurate, precise and effective comparable to HPLC for measuring total aflatoxins ranging from 4 to 40 ppb (Zheng et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Aflatoxin B1 Contamination Correlation with Aspergillus flavus in Animal Feed Raw Materials Imported through the Port of Tanjung Perak Surabaya, Indonesia

Arios¹,

Indrawati²,

Pamungkas³

2017

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oThis study aimed to measure the incidence rate of aflatoxin B1 contamination in feed raw materials (MBM and PPM) imported through Port of Tanjung Perak Surabaya, and its relation to the growth of the fungi that produce it. ELISA (Enzyme Linked Immunosorbent Assay) competitive was conducted to identify Aflatoxin B1 and followed by HPLC (High Performance Liquid Chromatography). Fungal identification was conducted as a macroscopic and microscopic observation. ELISA test showed 55 of 84 (55.95%) samples were positive for aflatoxin B1 contamination, while 3 of 7 (42.85%) samples tested by HPLC were positive. The result of fungal identification showed 1 of 12 samples positive Aspergillus flavus, and others types of fungi. AFB1 contamination in animal feed raw materials is likely to have occurred in the country of origin, whereas the growth of the fungus is due to the level of humidity and temperature during the transportation and storage process.

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“…Determination of aflatoxins by the HPLC method HPLC was used as reference method for the determination of aflatoxins in pistachio nuts [42]. Aflatoxins were isocratically separated using a HPLC (Waters model 2690), with a C-18 column (200 9 4.6 mm), a fluorescence detector and 10 lL of sample injection.…”

Section: Artificial Neural Networkmentioning

confidence: 99%

Application of gold-labeled antibody biosensor in simultaneous determination of total aflatoxins using artificial neural network

Saidi

Mirzaei

2013

J IRAN CHEM SOC

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Preparation of antibody-coated gold nanoparticles (GNPs) specific to aflatoxins B1, B2, G1 and G2 and its use in developing aflatoxins diagnostic method were presented in this paper. The formation of gold-labeled antibodies was accomplished at optimal condition. Due to severe overlapping between the emission profiles for the aflatoxins, they cannot be determined by direct inspection of data. The strategy used in this study, constituted by artificial neural network (ANN), was easy to implement and to originate reliable results. ANN can be successfully applied to spectrofluorimetric spectra matrices to simultaneous determination of total aflatoxins. Quantitative results obtained using ANN method for aflatoxins in pistachio nuts samples were compared to those obtained using the HPLC method. Obtained results using these two methods did not show significant differences.

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Immunoassay based on monoclonal antibody for aflatoxin detection in poultry feed

Cited by 62 publications

References 21 publications

Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oil

Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oil

Aflatoxin B1 Contamination Correlation with Aspergillus flavus in Animal Feed Raw Materials Imported through the Port of Tanjung Perak Surabaya, Indonesia

Application of gold-labeled antibody biosensor in simultaneous determination of total aflatoxins using artificial neural network

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