Preparation of antibody-coated gold nanoparticles (GNPs) specific to aflatoxins B1, B2, G1 and G2 and its use in developing aflatoxins diagnostic method were presented in this paper. The formation of gold-labeled antibodies was accomplished at optimal condition. Due to severe overlapping between the emission profiles for the aflatoxins, they cannot be determined by direct inspection of data. The strategy used in this study, constituted by artificial neural network (ANN), was easy to implement and to originate reliable results. ANN can be successfully applied to spectrofluorimetric spectra matrices to simultaneous determination of total aflatoxins. Quantitative results obtained using ANN method for aflatoxins in pistachio nuts samples were compared to those obtained using the HPLC method. Obtained results using these two methods did not show significant differences.
Infrared (IR) spectrophotometric determination of dioxathion in cattle dipwashes was checked for its suitability as a routine method. Two calibration curves constructed from absorbances of a series of solutions of dioxathion in carbon tetrachloride and a series of extracts of equivalent quantities of dioxathion from aqueous emulsions were similar (slope 1.8). Several determinations of a field sample gave a mean concentration of 292 ppm or 0.029% and a coefficient of variation of 1.6%. Two determinations of an aqueous emulsion of dioxathion of approximately 400 ppm gave concentrations of 440 and 404 ppm.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.