Immuno-histochemistry in the detection of early myocardial infarction (a post-mortem study)

Amin, Hebat Allah; El-Hennawy, Ahmed; Nakhla, Gina A.; Tabak, Sahar Abdel-Hamid; Hassan, Hanan Hosny

doi:10.1016/j.ejfs.2011.04.003

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Troponin is a common biomarker for cardiac injury; therefore, its degradation in myocardial samples has also been investigated in previous immunostaining-based studies. It was found that staining for cTnT and cardiac troponin I (cTnI) in human, canine, porcine, and rat heart muscle was absent or significantly decreased after myocardial infarction [ 36 , 37 ]. Similarly, a porcine heart failure model using Western blots showed an up to 70% reduction in cTnT and cTnI immunoreaction intensity at two months post-infarction [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Influencing Factors on Postmortem Protein Degradation for PMI Estimation: A Systematic Review

et al. 2021

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The present review provides an overview of the current research status on the effects of influencing factors on postmortem protein degradation used to estimate the PMI (postmortem interval). Focus was set on characteristics of internal and external influencing factors and the respective susceptibility and/or robustness of protein degradation. A systematic literature search up to December 2020 was conducted on the effect of influencing factors investigated in the context of postmortem protein degradation in the tissues of animals and humans using the scientific databases PubMed and Google Scholar, as well as the reference lists of eligible articles. We identified ten studies investigating a total of seven different influencing factors in degrading tissues/organs (n = 7) of humans and animals using six different methodological approaches. Although comparison of study outcomes was impeded by the high variety of investigated factors, and by high risk of bias appraisals, it was evident that the majority of the influencing factors concerned affected protein degradation, thus being able to modulate the precision of protein degradation-based PMI estimation. The results clearly highlight the need for a thorough screening for corresponding factors to enable the introduction of appropriate correction factors and exclusion criteria. This seems especially relevant for the protein degradation-based study of human PMI to increase the reliability and precision of the method and to facilitate a broader applicability in routine forensic casework.

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Section: Discussionmentioning

confidence: 99%

Influencing Factors on Postmortem Protein Degradation for PMI Estimation: A Systematic Review

et al. 2021

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“…Several studies in different experimental models showed that measurement of cTn in serum is the most sensitive and specific biomarker of myocardial necrosis (Thygesen et al, 2010). Furthermore, various observations suggested that cTn immunohistochemical staining might be useful for identifying myocardial necrosis in human hearts autopsy (Amin et al, 2011;Hansen and Rossen, 1999). Our study, in a minimally invasive canine model of MI, demonstrated that cTnI staining loss could be identified even in the absence of histologic evidence of necrosis such as in cardiac apex, i.e., no loss of cTnI was observed in non-necrotic myocardium, meanwhile, a great loss was observed in necrotic myocardium.…”

Section: Discussionmentioning

confidence: 99%

“…An exception was the myocardium of cardiac apex, where loss of cTnI was visible even in non-necrotic myocardium. The explanations for this is, distribution of cTnI flow within the ischemic tissue is not uniform, development of necrosis may occur earlier in some parts of infract than others, as well as flow of cTnI away from necrotic fibers may also be changeable, causing rate of "washout" difference in different fibers (Amin et al, 2011). So, our studies demonstrated that cTnI immunohistochemical staining may be more sensitive than usual Masson's trichrome staining for the recognition of myocardial necrosis in experimental animals as well as human hearts at autopsy.…”

Section: Discussionmentioning

confidence: 99%

A cardiac troponin I study in a minimally invasive myocardial infarction canine model

Mikrani¹,

Liang²,

Naveed³

et al. 2019

JAB

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Cardiac troponin I (cTnI) is an important biomarker of acute myocardial infarction (MI) in animals and human beings. Nevertheless, no immunohistochemical study has been reported about the pattern of myocardial cTnI egression in a minimally invasive model. The present study intended to establish a minimally invasive model of MI and to evaluate the distribution of cTnI. Twelve Mongrel dogs were divided into 2 groups (n = 6): experimental and sham-operated group. Three incisions were made on the left thoracic wall, left anterior descending (LAD) of coronary artery was identified and titanium nips were clamped by video-assisted thoracoscopy surgery (VATS). Series of electrocardiograms (ECG) and biochemical analyses of blood samples-oxidatively modified proteins (OMP), creatine kinase (CK), and cTnI were performed. Furthermore, Masson's trichrome staining was used to observe the histopathology of cardiac myocytes, while immunohistochemistry was done to observe cTnI egression from myocardium. ECG showed elevated ST-segment, whereas OMP, CK and cTnI level increased remarkably and declined to baseline subsequently in the model group throughout study period. Masson's trichrome staining of model group showed a large amount of collagen deposition in the fibrotic area as compared to control group. In immunohistochemical staining, no loss of cTnI staining was observed in non-necrotic myocardium, meanwhile, a great loss was observed in necrotic myocardium. An exception was the myocardium of cardiac apex, where loss of cTnI was visible even in non-necrotic myocardium. All these results revealed that loss of cTnI occurs not only in the necrotic myocardium but also in so-called non-necrotic myocardium of minimally invasive MI model through VATS.

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“…However; it is not the marker of choice that can be used in the detection of early myocardial ischemia in advanced autolytic changes - despite its sensitivity generally. Other antibodies that can withstand autolysis as troponin, myoglobin and desmin can do that role instead [ 5 , 11 , 17].…”

Section: Discussionmentioning

confidence: 99%

“…Loss of myocardial staining of H-FABP in the three groups was recorded. The obtained results were scored from (0) to (-3) according to the percent of myocardial fibers showing depletion of H-FABP staining: Score 0: no loss of H-FABP staining, Score -1: loss in less than 25% of fibers, Score -2: loss in 25-50% of fibers, Score -3: loss in >50% of fibers [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Role of Cardiac Myocytes Heart Fatty Acid Binding Protein Depletion (H-FABP) in Early Myocardial Infarction in Human Heart (Autopsy Study)

Shabaiek

Ismael²,

Elsheikh³

et al. 2016

Open Access Maced J Med Sci

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BACKGROUND:Many immunohistochemical markers have been used in the postmortem detection of early myocardial infarction.AIM:In the present study we examined the role of Heart-type fatty acid binding protein (H-FABP), in the detection of early myocardial infarction.MATERIAL AND METHODS:We obtained samples from 40 human autopsy hearts with/without histopathological signs of ischemia.RESULTS:All cases of definite and probable myocardial infarction showed a well-defined area of H-FABP depletion. All of the control cases showed strong H-FABP expression, except two markedly autolysed myocardial samples that showed affected antigenicity.CONCLUSION:Thus, we suggest H-FABP as being one of the valuable tools facing the problem of postmortem detection of early myocardial infarction/ischemia, but not in autolysis.

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Immuno-histochemistry in the detection of early myocardial infarction (a post-mortem study)

Cited by 9 publications

References 10 publications

Influencing Factors on Postmortem Protein Degradation for PMI Estimation: A Systematic Review

Influencing Factors on Postmortem Protein Degradation for PMI Estimation: A Systematic Review

A cardiac troponin I study in a minimally invasive myocardial infarction canine model

Role of Cardiac Myocytes Heart Fatty Acid Binding Protein Depletion (H-FABP) in Early Myocardial Infarction in Human Heart (Autopsy Study)

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