Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1

Setlem, Keerthana; Mondal, Bhairab; Ramlal, Shylaja; Kingston, Joseph J.

doi:10.3389/fmicb.2016.01909

Cited by 47 publications

(20 citation statements)

References 38 publications

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“…Compared to traditional SELEX, counter SELEX adds an additional step using structurally-similar targets to incubate with aptamers to effectively discriminate non-specific oligonucleotides. This procedure has been dramatically applied to other modified SELEX methods to obtain more specific aptamers [ 10 , 11 , 12 ]. It is to be noted that the prominent difference between counter SELES and negative SELEX is their use of different incubation objects.…”

Section: Limitations and Advancements In Aptamer Selectionmentioning

confidence: 99%

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

Zhang

Wang

et al. 2017

IJMS

350

205

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Aptamers are short DNA/RNA oligonucleotides capable of binding to target molecules with high affinity and specificity. The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks to the inherit merits, aptamers have been used in a wide range of applications, including disease diagnosis, targeted delivery agents and therapeutic uses. To date, great achievements regarding the selection, modifications and application of aptamers have been made. However, few aptamer-based products have already successfully entered into clinical and industrial use. Besides, it is still a challenge to obtain aptamers with high affinity in a more efficient way. Thus, it is important to comprehensively review the current shortage and achievement of aptamer-related technology. In this review, we first present the limitations and notable advances of aptamer selection. Then, we compare the different methods used in the kinetic characterization of aptamers. We also discuss the impetus and developments of the clinical application of aptamers.

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Section: Limitations and Advancements In Aptamer Selectionmentioning

confidence: 99%

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

Zhang

Wang

et al. 2017

IJMS

350

205

View full text Add to dashboard Cite

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“…In early 2015, a heightened risk of aflatoxin poisoning became a concern of the Provincial Government of East Java, Indonesia, due to the increase in small industries which commercially process and market animal feed traditionally without considering sanitation and/or product quality [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements

Lazuardi¹,

Hermanto²

2017

Vet World

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Aim:The objective of the current study is to determine the concentration of aflatoxin B1 using high-performance liquid chromatography (HPLC) with a photodiode array (PDA) detector.Materials and Methods:Aflatoxin B1 certified reference grade from Trilogy Analytical Laboratory dissolved acetonitrile (ACN) at 10 µg/mL was using standard assessment. HPLC instruments such as ultraviolet-PDA detector used a Shimadzu LC-6AD pump with DGU-20A5 degasser, communication module-20A, and PDA detector SPD-M20A with FRC-10A fraction collector. The HPLC was set isocratic method at 354 nm with a reverse-phase ODS C18 column (LiChrospher® 100 RP-18; diameter, 5 µm) under a 20°C controlled column chamber. Rheodyne® sample loops were performed in 20 µL capacities. The mobile phase was performed at fraction 63:26:11 H2O: methanol:ACN at pH 6.8. A total of 1 kg of feed contained 10% bread crumbs and 30% concentrated, 40% forage, and 20% soybean dregs were using commercials samples. Samples were extracted by ACN and separated with solid phase extraction ODS 1 mL than elution with mobile phase to collect at drying samples performed. The samples were ready to use after added 1 mL mobile phase than injected into the system of HPLC.Results:We found that the retention time of aflatoxin B1 was approximately 10.858 min. Linearity of 0.01-0.08 µg/mL aflatoxin B1 dissolved in mobile phase was obtained at R2=0.9. These results demonstrate that these methods can be used to analyze aflatoxin B1 and gain 89-99% recovery. The limit of detection of this assay was obtained at 3.5 × 10−6 µg/mL.Conclusion:This method was easy to apply and suitable to analyzing at small concentrations of aflatoxin B1 in formulated product of feed cattle.

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“…Since its launch in 1990, conventional SELEX has been progressively modified to improve aptamer specificity and affinity, to simplify the process and to increase time and cost efficiency [ 44 – 46 ]. The modifications in the SELEX method depend on the purpose and target molecules.…”

Section: Aptamer: Oligonucleotides Nanomedicinementioning

confidence: 99%

Aptamer: A potential oligonucleotide nanomedicine in the diagnosis and treatment of hepatocellular carcinoma

et al. 2017

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Hepatocellular carcinoma (HCC) is one of the most common cancers with a high mortality rate. Late diagnosis and poor prognosis are still a major drawback since curative therapies such as liver resection and liver transplantation are effective only for an early stage HCC. Development of novel molecular targeting therapies against HCC may provide new options that will improve the efficiency of the diagnosis and the success of the therapy, thus ameliorating the life expectancy of the patients. The aptamer is an oligonucleotide nanomedicine that has high binding affinity and specificity to small and large target molecules in the intracellular and extracellular environment with agonist or antagonist function. Currently, several aptamers for diagnostic and therapeutic purposes are under development to recognize different molecules of HCC. In in vitro models, the aptamer has been shown to be able to reduce the growth of HCC cells and increase the sensitivity to conventional chemotherapies. In in vivo mouse models, aptamer could induce cell apoptosis with antitumor activity. Overall data had shown that aptamer has limited toxicity and might be safe in clinical application. This review summarizes recent information of aptamer as a potential oligonucleotide nanomedicine tool, in diagnostics, targeted therapy, and as drug delivery nano-vehicles.

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Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1

Cited by 47 publications

References 38 publications

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

High-performance liquid chromatography ultraviolet-photodiode array detection method for aflatoxin B1 in cattle feed supplements

Aptamer: A potential oligonucleotide nanomedicine in the diagnosis and treatment of hepatocellular carcinoma

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