Immunization with recombinant Lactobacillus casei strains producing K99, K88 fimbrial protein protects mice against enterotoxigenic Escherichia coli

Liu, Wen; Hou, Xilin; Wang, Guihua; Yu, Liyun; XiaoMan, Wei; Liu, Jian‐Kui; Liu, Qinfang; Chunhua, Wei

doi:10.1016/j.vaccine.2011.08.036

Cited by 35 publications

(25 citation statements)

References 49 publications

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“…Mucosal immunity is likely to be involved as a first-line defense because ETEC primarily targets the intestinal tract (Moyle et al 2004). The mammalian mucosal immune response is characterized by an IgA-dominated antibody profile and the involvement of mucosal IgA in protecting against ETEC infection has been reported (Verdonck et al 2005;Wen et al 2012). In the present study, we observed induction of antigen-specific IgA in the feces of inoculated mice.…”

Section: Discussionsupporting

confidence: 73%

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Hur

Kim

et al. 2015

Veterinary Quarterly

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Results: All groups with single, double, and triple inoculations of the ghost showed higher humoral and mucosal immune responses than the control group. In particular, the groups with intramuscular double and triple inoculations showed significantly higher immune responses. In addition, oral inoculation with a combination of the ghost and MONTANIDE IMS 1113 (MI1113) resulted in high and prolonged induction of intestinal IgA levels. Conclusion: These results indicated that both systemic and mucosal immunity against ETEC fimbrial antigens expressed by the ghost are induced by intramuscular booster inoculation with the ghost, and that addition of M1113 to the ghost was found to result in prominent induction of mucosal immunity through oral inoculation.

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Section: Discussionsupporting

confidence: 73%

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Hur

Kim

et al. 2015

Veterinary Quarterly

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“…The recombinant probiotic was able to neutralize 93% of enterotoxin in culture lysates of diverse ETEC strains . Similarly, a recombinant L. casei strain carrying the K88 fimbriae from ETEC was able to reduce the attachment of ETEC to porcine intestinal brush border in a dose-dependent manner and to reduce infection in a mice model (Wen et al, 2012). These studies demonstrate that bioengineered probiotics can be used in the targeted control of specific enteropathogens.…”

Section: Wild-type and Bioengineered Probiotics To Control Foodborne mentioning

confidence: 70%

Modern Approaches in Probiotics Research to Control Foodborne Pathogens

Amalaradjou

Bhunia

2012

Advances in Food and Nutrition Research

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Foodborne illness is a serious public health concern. There are over 200 known microbial, chemical, and physical agents that are known to cause foodborne illness. Efforts are made for improved detection, control and prevention of foodborne pathogen in food, and pathogen associated diseases in the host. Several commonly used approaches to control foodborne pathogens include antibiotics, natural antimicrobials, bacteriophages, bacteriocins, ionizing radiations, and heat. In addition, probiotics offer a potential intervention strategy for the prevention and control of foodborne infections. This review focuses on the use of probiotics and bioengineered probiotics to control foodborne pathogens, their antimicrobial actions, and their delivery strategies. Although probiotics have been demonstrated to be effective in antagonizing foodborne pathogens, challenges exist in the characterization and elucidation of underlying molecular mechanisms of action and in the development of potential delivery strategies that could maintain the viability and functionality of the probiotic in the target organ.

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“…Recombinant probiotics can act as a vaccine arming the host immune system to deal with gut pathogens [141]. In order to exploit a safe and effective vaccine for the prevention against K99 infections of ETEC, Wen et al [142] cloned and expressed ETEC adhesins K99 into the probiotic L. casei . They reported that there was an increase in the efficacy of the recombinant probiotic and that more than 80% of the vaccinated mice were protected after challenge with a lethal dose of standard strains.…”

Section: The Concept Of Probiotic Bioengineering or Recombinant Probimentioning

confidence: 99%

Probiotic engineering: towards development of robust probiotic strains with enhanced functional properties and for targeted control of enteric pathogens

2017

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There is a growing concern about the increase in human morbidity and mortality caused by foodborne pathogens. Antibiotics were and still are used as the first line of defense against these pathogens, but an increase in the development of bacterial antibiotic resistance has led to a need for alternative effective interventions. Probiotics are used as dietary supplements to promote gut health and for prevention or alleviation of enteric infections. They are currently used as generics, thus making them non-specific for different pathogens. A good understanding of the infection cycle of the foodborne pathogens as well as the virulence factors involved in causing an infection can offer an alternative treatment with specificity. This specificity is attained through the bioengineering of probiotics, a process by which the specific gene of a pathogen is incorporated into the probiotic. Such a process will subsequently result in the inhibition of the pathogen and hence its infection. Recombinant probiotics offer an alternative novel therapeutic approach in the treatment of foodborne infections. This review article focuses on various strategies of bioengineered probiotics, their successes, failures and potential future prospects for their applications.

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Immunization with recombinant Lactobacillus casei strains producing K99, K88 fimbrial protein protects mice against enterotoxigenic Escherichia coli

Cited by 35 publications

References 49 publications

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Modern Approaches in Probiotics Research to Control Foodborne Pathogens

Probiotic engineering: towards development of robust probiotic strains with enhanced functional properties and for targeted control of enteric pathogens

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