Immune Status and Growth of Broiler Fed Diet with Microparticle Protein Added with Natural Acidifier

Abstract

The present study was conducted to evaluate the feeding effect of diet composed of microparticle protein added with lime juice as an acidifier on immune status and efficiency of protein utilization in relation to the growth of broiler chicken. Dietary treatments tested were P1 was diet with 20% intact protein and CaCO3 as a Ca source, P2 was diet with 20% microparticle protein and eggshell as a Ca source added with 1.2% lime juice, and P3 was diet with 20% microparticle protein and eggshell as a Ca source added with 2.4% lime juice. Variables measured were protein and amino acids digestibility, retentions of N and Ca, fecal Nτ–methyl histidine (Nτ–MH) excretion as a protein turnover index, lymphoid organs, heterophil and lymphocyte (H/L) ratio, immunoglobulin A (IgA) as a body resistance indicator, feed consumption, body weight gain (BWG), and feed conversion ratio (FCR). The experiment was arranged in a completely randomized design with 3 treatments and 8 replications. Data were subjected to analysis of variance and Duncan test at 5% probability. Protein and amino acid digestibility, N, and Ca retentions were significantly (p<0.05) higher in P2 and P3. However, the H/L ratio was significantly (p<0.05) lower in both treatments compared to P1. Bursa fabricius and lymph weights were not difference, but BWG in P2 significantly (p<0.05) increased. In conclusion, protein and some essential amino acids digestibility, N and Ca retentions, IgA, and BWG increase with the decrease in H/L ratio, fecal Nτ–MH, feed consumption, and FCR in broiler fed diet composed of microparticle protein source with additional 1.2 mL acidifier of lime juice.

Antioxidant Enzymes and Growth of Broiler Fed Microparticle Protein Diet with Inulin or Lactobacillus acidophillus Supplementation

“…It was possible that broiler-fed microparticle protein sources of fish meal and soybean meal increased protein digestibility and N retention (Suthama & Wibawa, 2018). Other reports also supported that protein and amino acid digestibility and Ca and N retentions increased in broiler fed diet composed of microparticle protein source added with acidifier (Suthama et al, 2021a). The increase in protein utilization efficiency in particular and feed in general is an important key in the broiler management industry as an economical meat producer.…”

“…Two hundred and forty (240) broilers (Cobb 500 strain) of 14-day old for treated animals and 10 birds for endogenous correction (average body weight was 268±30 g) were used in the current study. The birds since day-old until two weeks of age were provided a preliminary period that was divided into 2 steps as reported elsewhere (Suthama et al, 2021a). A commercial feed was fully given during the first week, and it was continued to a combination of commercial and experimental diets for another week thereafter.…”

“…Experimental feeds were composed of microparticle protein sources derived from fish and soybean meals. The microparticle protein processing method was based on the previous study of Suthama & Wibawa (2018) with an ultrasonic bath treatment system using an ultrasound transducer as the most recently reported (Suthama et al, 2021a).…”

“…leaf extract with a normal protein diet (Maesaroh et al, 2022). Feeding a microprotein diet with the inclusion of prebiotics or probiotic would be an ideal combination ensuring the host animal's health and productive efficiency in the absence of using antibiotic growth promoters (AGPs) (Suthama et al, 2021a, b). However, since the use of AGPs is prohibited, encouraging researchers to obtain and investigate beneficial natural feed additives with improving effects on poultry production without harming either host animal or consumer health.…”

Pengaruh Level dan Lama Pemberian Sinbiotik Ekstrak Porang dan Lactobacillus casei terhadap Retensi Nutrien dan Produktivitas Itik Tegal

“…Penyerapan kalsium dipengaruhi oleh keasaman saluran pencernaan. Protein dan Ca "selalu bersama" selama proses penyerapan (Suthama et al, 2021)…”