Immune responses of two recombinant adenoviruses expressing VP1 antigens of FMDV fused with porcine granulocyte macrophage colony-stimulating factor

Du, Yijun; Jiang, Ping; Li, Yufeng; He, Hairong; Jiang, Wenming; Wang, Xinglong; Wang, Hong

doi:10.1016/j.vaccine.2007.09.062

Cited by 19 publications

(4 citation statements)

References 50 publications

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“…The VP1 protein is the main antigenic protein in FMDV and can induce the production of protective neutralizing antibodies to provide a greater protective effect. Therefore, many studies of FMDV subunit vaccines have focused on the VP1 protein [ 25 – 29 ]. However, FMDV subunit vaccines have lower immunological effects than traditional inactivated vaccines [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

Liu

Fang

et al. 2017

BioMed Research International

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Improving vaccine immunogenicity by targeting antigens to dendritic cells has recently emerged as a new design strategy in vaccine development. In this study, the VP1 gene of foot-and-mouth disease virus (FMDV) serotype A was fused with the gene encoding human immunodeficiency virus (HIV) membrane glycoprotein gp120 or C2-V3 domain of hepatitis C virus (HCV) envelope glycoprotein E2, both of which are DC-SIGN-binding glycoproteins. After codon optimization, the VP1 protein and the two recombinant VP1-gp120 and VP1-E2 fusion proteins were expressed in Sf9 insect cells using the insect cell-baculovirus expression system. Western blotting showed that the VP1 protein and two recombinant VP1-gp120 and VP1-E2 fusion proteins were correctly expressed in the Sf9 insect cells and had good reactogenicity. Guinea pigs were then immunized with the purified proteins, and the resulting humoral and cellular immune responses were analyzed. The VP1-gp120 and VP1-E2 fusion proteins induced significantly higher specific anti-FMDV antibody levels than the VP1 protein and stronger cell-mediated immune responses. This study provides a new perspective for the development of novel FMDV subunit vaccines.

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Section: Discussionmentioning

confidence: 99%

Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

Liu

Fang

et al. 2017

BioMed Research International

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“…Although the correlation between FMDV NA titer and protective efficacy in cattle has been well established [32-34], previous studies have shown that the protection against FMD is related not only to humoral immunity, but also to cellular immunity [35-37]. Previous studies have also shown that swine immunized with recombinant adenovirus expressing FMDV VP1 were completely protected against virulent FMDV challenge, even though the NA (titer < 40) induced by recombinant adenovirus expressing FMDV VP1 was significantly lower than that in swine inoculated with inactivated FMD vaccine [38,39]. Also, other reports showed that swine or cattle immunized with adenovirus expressing the FMDV P1 protein were protected against virulent FMDV challenge at post-vaccination day 7, although an NA titer was undetectable [28,40].…”

Section: Discussionmentioning

confidence: 99%

Induction of protective immune response against both PPRV and FMDV by a novel recombinant PPRV expressing FMDV VP1

Yin

Chen

et al. 2014

Vet Res

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Peste des petits ruminants (PPR) and foot-and-mouth disease (FMD) are both highly contagious diseases of small domestic and wild ruminants caused by the PPR virus (PPRV) and the FMD virus (FMDV). In this study, a recombinant PPRV expressing the FMDV VP1 gene (rPPRV/VP1) was generated and FMDV VP1 expression did not impair replication of the recombinant virus in vitro and immunogenicity in inducing neutralizing antibody against PPR in goats. Vaccination with one dose of rPPRV/VP1 induced FMDV neutralizing antibody in goats and protected them from challenge with virulent FMDV. Our results suggest that the recombinant PPRV expressing the FMDV VP1 protein is a potential dual live vectored vaccine against PPRV and FMDV.

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“…DNA vaccines can also induce cellular immunity and humoral immunity, but, unlike DNA vaccines, recombinant viruses are more stable, versatile for manufacture, storage, and have no limitation that DNA vaccines remains low relative efficacy, requiring multiple boosts with high doses. Several virus vectors were used as FMDV antigen delivery, such as fowlpox virus, pseudorabies virus, adenovirus (Qian et al 2004;Zheng et al 2006;Du et al 2007). Among the virus vectors, BHV-1 provides many unique advantages.…”

Section: Discussionmentioning

confidence: 99%

Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model

et al. 2009

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Foot-and-mouth disease (FMD) and infectious bovine rhinotracheitis (IBR) are two important infectious diseases of cattle. Using bovine herpesvirus type 1 (BHV-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. In this study, a recombinant BHV-1 was constructed by inserting the synthetic FMDV (O/China/99) VP1 gene in the the gE locus of BHV-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phIE CMV) and bovine growth hormone polyadenylation (BGH polyA) signal. After homologous recombination and plaque purification, a recombinant virus named BHV-1/gE(-)/VP1 was acquired and identified. The immunogenicity was confirmed in a rabbit model by virus neutralization test and enzyme-linked immunosorbent assay (ELISA). The result indicated that the BHV-1/gE(-)/VP1 has the potential for being developed as a bivalent vaccine for FMD and IBR.

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Immune responses of two recombinant adenoviruses expressing VP1 antigens of FMDV fused with porcine granulocyte macrophage colony-stimulating factor

Cited by 19 publications

References 50 publications

Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

Induction of protective immune response against both PPRV and FMDV by a novel recombinant PPRV expressing FMDV VP1

Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model

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