Induction of protective immune response against both PPRV and FMDV by a novel recombinant PPRV expressing FMDV VP1

Yin, Chunsheng; Chen, Weiye; Hu, Qianqian; Wen, Zhiyuan; Wang, Xijun; Ge, Jinying; Yin, Qianqian; Hai-bing, Zhi; Xia, Chun; Bu, Zhigao

doi:10.1186/1297-9716-45-62

Cited by 19 publications

(16 citation statements)

References 36 publications

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“…Over the last several decades, the VP1 coding sequence has been partially or completely applied in vaccine design for the large-scale preparation of recombinant proteins in different expression systems, including E. coli, yeasts, baculovirus, plants, and mammalian cells (Chai et al 2013;Jung et al 2013;Lentz et al 2010;Li et al 2008;Parida et al 2006;Shi et al 2006) and thus FMDV VP1 was chosen for mucosal vaccine design in this study. The VP1 protein is the primary Ag inducing neutralizing Ab responses and plays a vital role in the assembly of FMDV particles (Yin et al 2014). Here, the sequence of VP1 was optimized according to the pool and codon used by L. lactis, by decreasing the GC content from 55.29-35.18%, without altering the protein sequence and optimizing unfavorable GC peaks, which could improve translational performance (Jiang et al 2014).…”

Section: Discussionmentioning

confidence: 99%

The immune response to a recombinant Lactococcus lactis oral vaccine against foot-and-mouth disease virus in mice

Liu

et al. 2020

Biotechnol Lett

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Objective Development of an effective mucosal vaccine to induce specific immune responses against Foot-and-mouth disease virus (FMDV). Results For this purpose, the FMDV VP1 gene (SPVP1) was optimized and synthesized based on the codon bias of Lactococcus lactis (L. lactis), and then incorporated in the plasmid pNZ8148. L. lactis NZ9000 containing the pNZ8148-SPVP1 recombinant plasmid was used as an oral delivery vehicle to induce anti-FMDV mucosal and systemic immune responses in mice. After confirmation that the SPVP1 protein was expressed successfully in the recombinant L. latic, the mice were orally challenged with NZ9000-pNZ8148, NZ9000-pNZ8148-SPVP1, phosphate-buffered saline as a mock infection group, or with inactivated vaccine as a positive group. Mice immunized with NZ9000-pNZ8148-SPVP1 produced high levels of mucosal secretory IgA (sIgA), antigenspecific serum IgG, IgA, and neutralizing antibodies, and developed stronger cell-mediated immune reactions and significant T spleen lymphocyte proliferation. Furthermore, the recombinant group generated much higher levels of IFN-c, IL-2, IL-4, IL-5, and IL-10 than the other groups. Conclusions Potent immune responses were successfully elicited in mice with FMDV VP1 delivered through L. lactis.Keywords Lactococcus lactis Á Oral vaccine Á Mucosal immunity Á Foot-and-mouth disease virus Á VP1 protein Xinsheng Liu and Linlin Qi have contributed equally to this study.

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Section: Discussionmentioning

confidence: 99%

The immune response to a recombinant Lactococcus lactis oral vaccine against foot-and-mouth disease virus in mice

Liu

et al. 2020

Biotechnol Lett

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“…In order to effectively contain PPR, prophylactic immunization for animals in areas where PPR infection was suspected was recommended (Liu, Wu, Zou, Li, & Wang, ; Yin et al, ). Live‐attenuated vaccines were used to impart immunity that could last a minimum of three years.…”

Section: Discussionmentioning

confidence: 99%

Spatio‐temporal analysis of peste des petits ruminants outbreaks in PR China (2013–2018): Updates based on the newest data

Gao

Liu

Zheng

et al. 2019

Transbound Emerg Dis

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Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants.The purpose of this study was to investigate the epidemic characteristics of PPR outbreaks in the People's Republic of China (PR China) from 2013 to 2018. A total of 41,876 PPR outbreaks were recorded in small ruminant populations in PR China during that period. Data from February to June 2018 were used to study new developments in the PPR epidemic in PR China. Spatio-temporal clusters and temporal distribution patterns were studied based on PPR notifications. We also used multiple logistic regression to examine the contribution of anthropogenic, climatic and topographic factors to PPR outbreaks. Distance to the nearest road (OR = 1.007 [95% CI: 1.001-1.014]), price of mutton (OR = 1.904 [95% CI: 1.358-2.668]) and mean monthly temperature in July (OR 1.156 = [95% CI: 1.110-1.204]) showed positive effects on PPR outbreaks. Negative effects were observed for number of large-scale farms (OR = 0.962 [95% CI: 0.940-0.985]). We also found that observed patterns of seasonality were characterized by peaks in April of 2014. Spatio-temporal clusters occurred in Yunnan, Jiangsu, Anhui, Heilongjiang province and Chongqing municipality. Hunan province reported PPR occurrences every year from 2014 to June 2018. Yunnan, Jiangsu and Anhui province have 56, 33 and 30 epidemic locations, respectively. PPR infections were first reported as the cause of death for 19 wild bharals in Qinghai province in 2018. All of this suggests that domestic trading of sheep and goats may be closely related to the spread of PPR. Prophylactic immunization in suspected animal populations or areas is recommended for the control of PPR and wild small ruminants should be monitored. Results presented here provide improved knowledge about PPR dynamics in PR China, which could be helpful in designing more effective prevention strategies. K E Y W O R D S disease control, peste des petits ruminants, PR China, spatio-temporal cluster, statistical analysis, surveillance

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“…The gene used in this study, VP1 gene, has been the hot area of research in molecular biology of FMDV for years. VP1 protein exposed on the surface of viral particles is the main antigen of neutralizing antibody responses and plays an important part in forming the virus particles [41]. Over the past decades, the partial or complete VP1 coding sequence has been used in the development of engineering vaccines accommodating the large-scale production of recombinant protein, efficiently produced and expressed in various systems, such as E. coli, transgenic plants, yeasts, baculovirus and mammalian cells [19,[42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Protection against Foot-and-Mouth Disease Virus in Guinea Pigs via Oral Administration of Recombinant Lactobacillus plantarum Expressing VP1

et al. 2015

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Mucosal vaccination is an effective strategy for generating antigen-specific immune responses against mucosal infections of foot-and-mouth disease virus (FMDV). In this study, Lactobacillus plantarum strains NC8 and WCFS1 were used as oral delivery vehicles containing a pSIP411-VP1 recombinant plasmid to initiate mucosal and systemic immune responses in guinea pigs. Guinea pigs were orally vaccinated (three doses) with NC8-pSIP411, NC8-pSIP411-VP1, WCFS1-pSIP411, WCFS1-pSIP411-VP1 or milk. Animals immunized with NC8-pSIP411-VP1 and WCFS1-pSIP411-VP1 developed high levels of antigen-specific serum IgG, IgA, IgM, mucosal secretory IgA (sIgA) and neutralizing antibodies, and revealed stronger cell-mediated immune responses and enhanced protection against FMDV challenge compared with control groups. The recombinant pSIP411-VP1 effectively improved immunoprotection against FMDV in guinea pigs.

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Induction of protective immune response against both PPRV and FMDV by a novel recombinant PPRV expressing FMDV VP1

Cited by 19 publications

References 36 publications

The immune response to a recombinant Lactococcus lactis oral vaccine against foot-and-mouth disease virus in mice

The immune response to a recombinant Lactococcus lactis oral vaccine against foot-and-mouth disease virus in mice

Spatio‐temporal analysis of peste des petits ruminants outbreaks in PR China (2013–2018): Updates based on the newest data

Protection against Foot-and-Mouth Disease Virus in Guinea Pigs via Oral Administration of Recombinant Lactobacillus plantarum Expressing VP1

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