Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model

Abstract

Foot-and-mouth disease (FMD) and infectious bovine rhinotracheitis (IBR) are two important infectious diseases of cattle. Using bovine herpesvirus type 1 (BHV-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. In this study, a recombinant BHV-1 was constructed by inserting the synthetic FMDV (O/China/99) VP1 gene in the the gE locus of BHV-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phIE CMV) and bovine growth hormone polyadenylation (BGH polyA) signal. After homologous recombination and plaque purification, a recombinant virus named BHV-1/gE(-)/VP1 was acquired and identified. The immunogenicity was confirmed in a rabbit model by virus neutralization test and enzyme-linked immunosorbent assay (ELISA). The result indicated that the BHV-1/gE(-)/VP1 has the potential for being developed as a bivalent vaccine for FMD and IBR.

Foot-and-mouth disease vaccines: recent updates and future perspectives

“…A bovine enterovirus expressing an FMDV epitope was also generated, but was not tested in a challenge experiment [31]. In a rabbit model, a recombinant bovine herpesvirus-1 displaying the FMDV VP1 gene induced a high level of neutralizing antibodies [95].…”