Immune responses of chickens inoculated with a recombinant fowlpox vaccine coexpressing HA of H9N2 avain influenza virus and chicken IL-18

Chen, Hongying; Shang, Yue; Yao, Huixia; Bao-an, Cui; Zhang, Hongying; Wang, Zixin; Wang, Ya-Dan; Chao, An-Jun; TingYun, Duan

doi:10.1016/j.antiviral.2011.04.007

Cited by 35 publications

(21 citation statements)

References 38 publications

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“…APS can induce the expression of surface antigens on lymphocytes, promote the production of antibodies, affect the secretion of cytokines, and even stimulate cell proliferation [17, 18]. Previous studies proved the effective immunostimulatory roles of APS against various viruses [17, 19, 20]. …”

Section: Introductionmentioning

confidence: 99%

Astragalus Polysaccharide Protects Astrocytes from Being Infected by HSV‐1 through TLR3/NF‐κB Signaling Pathway

Shi

Yin

Xin³

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Astragalus polysaccharide (APS) is the most immunoreactive substance in Astragalus. APS can regulate the body's immunity and is widely used in many immune related diseases. However, till now, there is little information about its contribution to the protection of astrocytes infected by virus. Toll-like receptor 3 (TLR3) is a key component of the innate immune system and has the ability to detect virus infection and trigger host defence responses. This study was undertaken to elucidate the protective effect of APS on herpes simplex virus (HSV-1) infected astrocytes and the underlying mechanisms. The results showed that APS protected the astrocytes from HSV-1 induced proliferation inhibition along with increasing expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) markedly. Moreover, APS significantly promoted the expression of Toll-like receptor 3 (TLR3) and the activation of nuclear factor-κB (NF-κB) in astrocytes. In addition, while astrocytes were pretreated with TLR3 antibody before adding HSV-1 and APS, the expression of TLR3, TNF-α, and IL-6 and the activation of NF-κB decreased sharply. These results indicate that APS can protect astrocytes by promoting immunological function provoked by HSV-1 through TLR3/NF-κB pathway.

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Section: Introductionmentioning

confidence: 99%

Astragalus Polysaccharide Protects Astrocytes from Being Infected by HSV‐1 through TLR3/NF‐κB Signaling Pathway

Shi

Yin

Xin³

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Currently, vaccination is one of the most promising control measures for H9N2 AIV. Unvaccinated chickens can become infected with modified live H9N2 virus strains used for early immunization [4], while inactivated vaccines have been shown to be safe and efficacious against H9N2 AIV [5]. Novel immune adjuvants are still in development and their use is highly desirable for producing vaccines which evoke a rapid immune response against viruses.…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study, it was reported that APS possess effective immunostimulatory effects when used in vaccination programs against Foot and mouth disease virus (FMDV), Newcastle disease virus (NDV) and Infectious bursal disease virus (IBDV) [5,8]. The appropriate dose of APS was likely to increase the expression of MHC class II, CD40, and CD86, and improve FMDV antigen-presentation during the early stages of the immune response [8].…”

Section: Introductionmentioning

confidence: 99%

Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo

Kallon

et al. 2013

J Animal Sci Biotechnol

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This study investigated the humoral immunization of Astragalus polysaccharide (APS) against H9N2 avian influenza virus (H9N2 AIV) infection in chickens.The effects of APS treatment on H9N2 infection was evaluated by an MTT [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens.

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“…Peripheral blood lymphocyte proliferation assay was performed according to a previous described method (6). Briefly, blood samples from all piglets were collected at 21 days after the boost immunization.…”

Section: Peripheral Blood Lymphocyte Proliferation Assaymentioning

confidence: 99%

Immune Responses of Piglets Immunized by a Recombinant Plasmid Containing Porcine Circovirus Type 2 and Porcine Interleukin-18 Genes

Chen

Wang

et al. 2014

Viral Immunology

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In this study, two recombinant plasmids containing the ORF2 gene of porcine circovirus type 2 (PCV2) with or without porcine interleukin-18 (IL-18) were constructed and evaluated for their ability to protect piglets against PCV2 challenge. Transient expression of the plasmids in PK-15 cells could be detected using Western blot. Piglets were given two intramuscular immunizations 3 weeks apart and were challenged with a virulent Wuzhi strain of PCV2 at 42 days after the initial immunization. All animals vaccinated with pBudCE4

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Immune responses of chickens inoculated with a recombinant fowlpox vaccine coexpressing HA of H9N2 avain influenza virus and chicken IL-18

Cited by 35 publications

References 38 publications

Astragalus Polysaccharide Protects Astrocytes from Being Infected by HSV‐1 through TLR3/NF‐κB Signaling Pathway

Astragalus Polysaccharide Protects Astrocytes from Being Infected by HSV‐1 through TLR3/NF‐κB Signaling Pathway

Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo

Immune Responses of Piglets Immunized by a Recombinant Plasmid Containing Porcine Circovirus Type 2 and Porcine Interleukin-18 Genes

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