This study investigated the humoral immunization of Astragalus polysaccharide (APS) against H9N2 avian influenza virus (H9N2 AIV) infection in chickens.The effects of APS treatment on H9N2 infection was evaluated by an MTT [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens.

Hypothalamic gonadotropin-releasing hormone (GnRH) is a major regulator of follicle-stimulating hormone (FSH) secretion in gonadotrope cell in the anterior pituitary gland. microRNAs (miRNAs) are small RNA molecules that control gene expression by imperfect binding to the 3′-untranslated region (3′-UTR) of mRNA at the post-transcriptional level. It has been proven that miRNAs play an important role in hormone response and/or regulation. However, little is known about miRNAs in the regulation of FSH secretion. In this study, primary anterior pituitary cells were treated with 100 nM GnRH. The supernatant of pituitary cell was collected for FSH determination by enzyme-linked immunosorbent assay (ELISA) at 3 hours and 6 hours post GnRH treatment respectively. Results revealed that GnRH significantly promoted FSH secretion at 3 h and 6 h post-treatment by 1.40-fold and 1.80-fold, respectively. FSHβ mRNA at 6 h post GnRH treatment significantly increased by 1.60-fold. At 6 hours, cells were collected for miRNA expression profile analysis using MiRCURY LNA Array and quantitative PCR (qPCR). Consequently, 21 up-regulated and 10 down-regulated miRNAs were identified, and qPCR verification of 10 randomly selected miRNAs showed a strong correlation with microarray results. Chromosome location analysis indicated that 8 miRNAs were mapped to chromosome 12 and 4 miRNAs to chromosome X. Target and pathway analysis showed that some miRNAs may be associated with GnRH regulation pathways. In addition, In-depth analysis indicated that 10 up-regulated and 3 down-regulated miRNAs probably target FSHβ mRNA 3′-UTR directly, including miR-361-3p, a highly conserved X-linked miRNA. Most importantly, functional experimental results showed that miR-361-3p was involved in FSH secretion regulation, and up-regulated miR-361-3p expression inhibited FSH secretion, while down-regulated miR-361-3p expression promoted FSH secretion in pig pituitary cell model. These differentially expressed miRNAs resolved in this study provide the first guide for post-transcriptional regulation of pituitary gonadotrope FSH secretion in pig, as well as in other mammals.

To determine the effect of astragalus and ginseng polysaccharides (APS, GPS) on immune response and improvement of H5N1 vaccine, 360-day-old broilers were randomly divided into 8 groups of 45 chicks, comprising APS groups (1–3); GPS groups (4–6); vaccine group (7); and blank control (8) (without polysaccharide and vaccine). From day 12 after hatch groups 1–3 were given APS and groups 4–6 with GPS both at 100, 200, and 400 (mg/kg), respectively. At day 15 after hatch, groups 1–7 were vaccinated with 0.3 mL H5N1 vaccine subcutaneously; daily weight gain (DWG) and serum Ig antibody (by HI-test) were measured on 3, 7, 14, and 28 days after vaccination. Serum antibody titers and expression of cytokines (IL-2, IL-10, I FN-γ, and TNF) were determined by ELISA and RT-PCR. Results revealed that all the polysaccharide groups were numerically increased in antibody levels and the expression of cytokines was significant (P < 0.05) in the APS and GPS groups compared to corresponding vaccine group and blank control. DWG was higher (P < 0.05) in 400 mg/kg APS groups than control groups. Thus oral supplements of GPS and APS have shown their potential in the improvement of immune response and could be used as adjuvant in a formulation of H5N1 vaccine.

The poultry industry worldwide suffers significantly due to climate change, which may affect the chicken protein source. The study examined backyard poultry farmers' awareness, perceptions, and responses to climate change in Tonkolili, Sierra Leone. Data were collected from 232 backyard poultry farmers using a semi-structured questionnaire. The data were analyzed using SPSS 23.0 Software. According to the respondents' socioeconomic profiles, the majority of farmers (53.4%) were between the ages of 31 and 40 years, and most of them were female (59.5%) and married (54.3%). The average household size was between 5-8 persons, and the majority of respondents (59.5%) lacked formal education. The poultry farmers are well aware of climate change (96.6%), and that decrease in rainfall (x̄ = 3.45) and increasing temperature (x̄ = 3.39) affects poultry production, leading to high incidences of poultry diseases (x̄ = 4.11), increases the cost of feed (x̄ = 3.99), and reduces the quantity of egg production (x̄ = 3.92). The logistic regression model results showed that the positive and significant factors that influenced climate change awareness are gender and sources of climate change information. Some of the adaptation strategies practices by the farmers are an adequate supply of drinking water, increased quantity of supplementary feed, and rearing a different variety of birds. Climate change affects backyard poultry production therefore, poultry farmers should be provided with technical training and assistance through extension services to help alleviate the impact of climate change on backyard poultry production.

Slaughterhouse workers are more susceptible to zoonotic disease infections from cuts and bloodletting, spreading blood-borne pathogens to societies. Adopting biosecurity measures by slaughterhouse workers in their daily tasks can lower the risk of spreading zoonotic diseases while improving food safety. This study aims to examine slaughterhouse workers' awareness of biosecurity, their perception of the efficiency of biosecurity measures in reducing zoonotic infections, and, most crucially, their adoption of such measures. The research was carried out in Sierra Leone's Northern Province's Koinadugu district. A total of 18 slaughterhouses were randomly selected in six chiefdoms of the Koinadugu district. A semi-structured questionnaire was used to survey 87 slaughterhouse workers in the 18 slaughterhouses selected in the six chiefdoms. The data were analyzed using Microsoft Excel version 2016 software. Results show that most slaughterhouse workers (87.4%) are well aware of biosecurity measures. They perceived cleaning and disinfecting the materials and equipment used in the slaughterhouse (x̄ = 4.43), as well as the workplace itself (x̄ = 4.33), and receiving proper training (x̄ = 4.33) as crucial biosecurity measures. The results also reveal little use of personal protective equipment by slaughterhouse workers (27%). There is low adoption of biosecurity measures by slaughterhouse workers in the district. Corruption and inadequate funding of relevant institutions affect the enforcement of biosecurity legislation in the district. Therefore, education and training programs can improve slaughterhouse workers' knowledge and understanding of biosecurity measures. Also, good governance should be fostered at all institutional levels to increase the effectiveness and efficiency of public sector service delivery

Climate change is having a negative influence on agriculture and livestock production systems. This study aims to assess livestock farmers’ perceptions and adaptive responses to climate change in the Koinadugu area of northern Sierra Leone. A sample of 126 livestock farmers was selected from four chiefdoms. The data were collected using a semi-structured questionnaire and meteorological time series data. The Statistical Package for Social Sciences (SPSS) version 23.0 and excel were used to analyze the data. According to the respondents’ socioeco­nomic characteristics, most farmers (93.7%) were males between 46 and 55 years. The majority of the farmers (58.7%) were married, and family sizes ranged from 5-8 individuals per household. Most of the livestock farmers (59.5%) in the study area had no formal education. Farmers attested to their awareness of climate change by essential indications such as increasing heat waves (77.0%), decreased rainfall (54.0%), and an increase in humidity (82.5%). Feed shortages (x̄ = 4.14), water scarcity (x̄ = 4.10), and higher veterinary care expenditures (x̄ = 4.25) are some of the impacts of climate change on livestock production. Numerous adaptation strategies include livestock reduction (87.3%), animal vaccination (95.2%), and Migration along with animals during the adverse climatic condition (75.4%), which the farmers employ. Lack of capital, inadequate information about climate change, and inadequate infrastructure impede adaptation. The study sug­gests that the government pursues strong policies to help livestock farmers adjust to climate unpredictability in the long run.

Slaughterhouse workers are more susceptible to zoonotic disease infections from cuts and bloodletting, spreading blood-borne pathogens to societies. Adopting biosecurity measures by slaughterhouse workers in their daily tasks can lower the risk of spreading zoonotic diseases while improving food safety. This study aims to examine slaughterhouse workers' awareness of biosecurity, their perception of the efficiency of biosecurity measures in reducing zoonotic infections, and, most crucially, their adoption of such measures. The research was carried out in Sierra Leone's Northern Province's Koinadugu district. A total of 18 slaughterhouses were randomly selected in six chiefdoms of the Koinadugu district. A semi-structured questionnaire was used to survey 87 slaughterhouse workers in the 18 slaughterhouses selected in the six chiefdoms. The data were analyzed using Microsoft Excel version 2016 software. Results show that most slaughterhouse workers (87.4%) are well aware of biosecurity measures. They perceived cleaning and disinfecting the materials and equipment used in the slaughterhouse (x̄ = 4.43), as well as the workplace itself (x̄ = 4.33), and receiving proper training (x̄ = 4.33) as crucial biosecurity measures. The results also reveal little use of personal protective equipment by slaughterhouse workers (27%). There is low adoption of biosecurity measures by slaughterhouse workers in the district. Corruption and inadequate funding of relevant institutions affect the enforcement of biosecurity legislation in the district. Therefore, education and training programs can improve slaughterhouse workers' knowledge and understanding of biosecurity measures. Also, good governance should be fostered at all institutional levels to increase the effectiveness and efficiency of public sector service delivery.

The physico-chemical and functional characteristics of peanut protein isolates extracted from peanut cakes obtained as by-products from two different peanut oil extraction methods (cold pressed and heat pressed) were studied. Isolates from cold pressed method (CPI) exhibited superior physical properties such as particle size (as seen from scanning electron micrographs), and thermal denaturation profiles as obtained from differential scanning calorimeter, compared to isolates obtained from heat pressed method (HPI). Solubility profiles obtained for CPI and HPI were pH dependent with CPI been more soluble at all pH levels. Solubility around the isoelectric pH range of 4.5-5.5 was low for both samples. Other functional properties such as emulsification, whipping, fat and water absorption were better exhibited by CPI than HPI. From the SE-HPLC, HPI recorded a higher molecular weight (16.64KDa) than CPI (14.91KDa), while CPI had higher biochemical components (protein, carbohydrates) than HPI.