Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo

Kallon, Sanpha; Li, Xiaorong; Ji, Jun; Chen, Cuiying; Xi, Qianyun; Chang, Shuang; Xue, Chunyi; Ma, Jingyun; Xie, Qingmei; Zhang, Youngliang

doi:10.1186/2049-1891-4-22

Cited by 65 publications

(58 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…APS, as an effective Chinese medicine extraction from Astragalus, has been shown anti-oxidation, anti-inflammatory, anti-antiviral activity [28]. Most researchers confirmed that APS took antiviral action only by strengthening the immune system [29]. The current work is the first to investigate the effect of APS on PCV2 replication in vitro.…”

Section: Discussionmentioning

confidence: 77%

Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway

Xue

Gan

Zhang

et al. 2015

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 77%

Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway

Xue

Gan

Zhang

et al. 2015

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

“…GPS was extracted and purified as described in our previous works [15, 16]. Trizol, isopropanol, chloroform [trichloromethane], DEPC water, and 75% ethanol were used in the extraction of RNA.…”

Section: Methodsmentioning

confidence: 99%

Vaccination with Astragalus and Ginseng Polysaccharides Improves Immune Response of Chickens against H5N1 Avian Influenza Virus

Abdullahi

Kallon

et al. 2016

BioMed Research International

Self Cite

View full text Add to dashboard Cite

To determine the effect of astragalus and ginseng polysaccharides (APS, GPS) on immune response and improvement of H5N1 vaccine, 360-day-old broilers were randomly divided into 8 groups of 45 chicks, comprising APS groups (1–3); GPS groups (4–6); vaccine group (7); and blank control (8) (without polysaccharide and vaccine). From day 12 after hatch groups 1–3 were given APS and groups 4–6 with GPS both at 100, 200, and 400 (mg/kg), respectively. At day 15 after hatch, groups 1–7 were vaccinated with 0.3 mL H5N1 vaccine subcutaneously; daily weight gain (DWG) and serum Ig antibody (by HI-test) were measured on 3, 7, 14, and 28 days after vaccination. Serum antibody titers and expression of cytokines (IL-2, IL-10, I FN-γ, and TNF) were determined by ELISA and RT-PCR. Results revealed that all the polysaccharide groups were numerically increased in antibody levels and the expression of cytokines was significant (P < 0.05) in the APS and GPS groups compared to corresponding vaccine group and blank control. DWG was higher (P < 0.05) in 400 mg/kg APS groups than control groups. Thus oral supplements of GPS and APS have shown their potential in the improvement of immune response and could be used as adjuvant in a formulation of H5N1 vaccine.

show abstract

“…Astragalus was used as supplementary forage supplement for livestock and poultry industry in China, because it contains polysaccharides, saponins, flavonoids, anthraquinones, alkaloids, amino acid, β-sitosterol and metallic elements (Ibrahim et al, 2013;Sun et al, 2013;Li et al, 2009). Recent literatures reported that crude extracts of Astragalus are observed to be antiinflammatory (Kim et al, 2013), immunostimulant (Qin et al, 2012), antioxidative (Kim and Yang, 2005), and antiviral in isolated constituents (Kallon et al, 2013). Astragalus was fermented by lactic acid bacteria (LAB) using solid state fermentation (SSF) technology.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Bacillus subtilis HNMY-13 and HNMY-15 strains in aflatoxin B1 degradation and Astragalus bio-transformation

Qiao¹,

Shi²,

Zhang³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Astragalus of traditional Chinese medicine (TCM) and Bacillus subtilis are extensively used in animal feed and for production of various fermented compounds. In order to observe the characteristics of HNMY-13 and HNMY-15 B. subtilis strains, this study focused on the optimization of parameters in liquid fermentation of the two strains in aflatoxin B 1 degradation, and Astragalus polysaccharide yield. The optimal parameters: degradation of aflatoxin B 1 , the bacterial count, and the Astragalus polysaccharide yield analysis were examined in this study. It was shown that the percentage degradation of AFB 1 by HNMY-13 and HNMY-15 strains were 80.48 and 79.55% at 37°C, and 80.66% and 81.34 with pH 7.0. The percentage degradation by HNMY-13 was 88.07% after 48 h fermentation and by HNMY-15 was 84.81% after 60 h fermentation, respectively. The viable count of fermented sample was 8.8×10 8 and 7.6×10 8 CFU·mL -1 using HNMY-13 and HNMY-15 strains, respectively. The polysaccharide yielded by HNMY-13 was 4.93% at 48 h which is 1.7-fold higher than that of the control group, while polysaccharide yielded by HNMY-15 reached peak of 3.54% at 60 h. In conclusion, HNMY-13 and HNMY-15 of B. subtilis promote production of Astragalus polysaccharide in the process of liquid fermentation and can also degrade aflatoxin B 1 significantly; hence the combination could form a potential feed additive for animals.

show abstract

Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo

Cited by 65 publications

References 23 publications

Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway

Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway

Vaccination with Astragalus and Ginseng Polysaccharides Improves Immune Response of Chickens against H5N1 Avian Influenza Virus

Characteristics of Bacillus subtilis HNMY-13 and HNMY-15 strains in aflatoxin B1 degradation and Astragalus bio-transformation

Contact Info

Product

Resources

About