Immune response in the adipose tissue of lean mice infected with the protozoan parasite<i>Neospora caninum</i>

Teixeira, Luzia; Moreira, João; Melo, Joana; Bezerra, Filipa; Marques, Raquel M.; Ferreirinha, Pedro; Correia, Alexandra; Monteiro, Mariana P.; Ferreira, Paula; Vilanova, Manuel

doi:10.1111/imm.12440

Cited by 18 publications

(45 citation statements)

References 62 publications

(131 reference statements)

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“…When we separate the data accordingly to previous exposure to N. caninum, as assessed by serology, an increased frequency in macrophages was observed in SAT of animals seropositive to N. caninum. These may suggest that in bovines, as already described in the murine model 53 , a previous infection challenge can affect the frequency of cellular populations in adipose tissue. On the other hand, animals seropositive to N. caninum showed lower frequencies of the CH138A + CD11b − MHC-II − CD45 − population in MAT and SAT, which further hints for an influence of infection in the bovine adipose tissue cell composition.…”

Section: Discussionmentioning

confidence: 56%

“…The frequency of cells in adipose tissue can be affected by several factors; one of those can be previous exposure to infection 53,69,70 . When we separate the data accordingly to previous exposure to N. caninum, as assessed by serology, an increased frequency in macrophages was observed in SAT of animals seropositive to N. caninum.…”

Section: Discussionmentioning

confidence: 99%

“…comparative analysis of myeloid cell populations in the adipose tissue of animals seropositive or seronegative for Neospora caninum. It has been shown in the murine model that N. caninum can impact on the frequencies of immune populations present in adipose tissue 12,53 . Since N. caninum infection is highly prevalent in Portuguese bovine herds, where seropositivity to this parasite can reach 80% 54 , we reanalysed our data splitting the animals in seropositive and seronegative to N. caninum.…”

Section: Cd45 Negative Cells In Bovine Adipose Tissue Flow Cytometrymentioning

confidence: 99%

“…We chose this kit to assess seropositivity to N. caninum as others have shown that this was one of the best-adjusted ELISA kit for the serological diagnosis of bovine neosporosis from the ones commercially available 74 . RnA isolation and real time pcR analysis. Total RNA extraction and cDNA synthesis were performed by a protocol similar to the one previously described in the murine model 53 . Briefly, RNA was extracted from total SVF cells (6 × 10 4 cells), total PBL (6 × 10 4 cells) and from sorted SSC-A high CD11b −/+ CD14 − MHC-II − C H138A − CD45 + cells (mast cells; 4 × 10 3 −1 × 10 4 sorted cells), isolated as described above, using TriReagent ™ (Sigma-Aldrich) according to the manufacturer's instructions.…”

Section: Immunocytochemistry Cytospins Of Svf Cells Isolated From Mamentioning

confidence: 99%

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Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Oliveira

Pinto

Correia

et al. 2020

Sci Rep

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immune cells resident in adipose tissue have important functions in local and systemic metabolic homeostasis. Nevertheless, these immune cell populations remain poorly characterized in bovines. Recently, we described diverse lymphocyte subpopulations in adipose tissue of Holstein-Friesian cows. Here, we aimed at characterising myeloid cell populations present in bovine adipose tissue using multicolour flow cytometry, cell sorting and histochemistry/immunohistochemistry. Macrophages, CD14 + CD11b + MHc-ii + CD45 + cells, were identified in mesenteric and subcutaneous adipose tissue, though at higher proportions in the latter. Mast cells, identified as SSC-A high CD11b −/+ CD14 − MHcii − CH138A − CD45 + cells, were also observed in adipose tissue and found at higher proportions than macrophages in mesenteric adipose tissue. Neutrophils, presenting a CH138A + CD11b + phenotype, were also detected in mesenteric and subcutaneous adipose tissue, however, at much lower frequencies than in the blood. Our gating strategy allowed identification of eosinophils in blood but not in adipose tissue although being detected by morphological analysis at low frequencies in some animals. A population not expressing CD45 and with the CH138A + CD11b − MHc-ii − phenotype, was found abundant and present at higher proportions in mesenteric than subcutaneous adipose tissue. The work reported here may be useful for further studies addressing the function of the described cells.www.nature.com/scientificreports www.nature.com/scientificreports/ colour-flow cytometry revealed the presence of CD14 + cells, CD172a + cells, CD11c + cells and CD163 + cells in the stromal vascular fraction (SVF) of subcutaneous and omental adipose tissue 23,24 . Recently, Aylward et al. 25 reported the presence of CD172a + CD11b + cells, CD11c + MHC-II + cells, and CD11b + CD11c + cells in mesenteric adipose tissue. However, these markers are not exclusive to macrophages but common to other myeloid cell populations or can be even expressed in non-hematopoietic cells. As an example, studies in humans and mice have shown that CD14 can be expressed in non-myeloid cells 26 such as preadipocytes 27 and epithelial cells 28 . Also, CD172a is expressed by granulocytes such as eosinophils 29 and mast cells 30 beside its expression in monocytes/ macrophages. Since mast cells 31,32 and eosinophils 3,33 have already been described in adipose tissue, single markers analysis limits the interpretation of the data. Therefore, to characterize the different myeloid cell populations in bovine adipose tissue we used a multicolour flow cytometry panel associated with cell sorting and histochemistry/immunohistochemistry analysis. Our strategy allowed identification and isolation of macrophages, mast cells and neutrophils with macrophages being observed at different proportions in mesenteric adipose tissue (MAT) and subcutaneous adipose tissue (SAT). We also showed that CD45 negative cells represent more than 40% of all the cells in the adipose tissue SVF of MAT. A non-leukocyte populat...

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Cd45 Negative Cells In Bovine Adipose Tissue Flow Cytometrymentioning

confidence: 99%

Section: Immunocytochemistry Cytospins Of Svf Cells Isolated From Mamentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Oliveira

Pinto

Correia

et al. 2020

Sci Rep

Self Cite

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“…Livers were fixed in buffered formalin and embedded in paraffin for hematoxylin-eosin (HE) histopathologic analysis or Foxp3 detection by immunohistochemistry as previously described (23). For histopathologic analysis of WT and Il10 Ϫ/Ϫ mice, five micrographs at ϫ100 magnification, from five different liver lobes, were randomly taken from each tissue slide sample.…”

Section: Il10mentioning

confidence: 99%

Poly- N -Acetylglucosamine Production by Staphylococcus epidermidis Cells Increases Their In Vivo Proinflammatory Effect

et al. 2016

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Staphylococcus epidermidis is a commensal bacterium of human skin and mucous membranes (1). The ability to establish biofilms on the surfaces of indwelling medical devices is a major virulence mechanism of this bacterium, which is the most commonly isolated etiological agent of nosocomial infections (2). S. epidermidis biofilm formation involves initial cellular adherence to a surface, followed by intercellular aggregation and accumulation in multilayer cell clusters (1). This process is dependent on adhesive extracellular molecules (3) such as poly-N-acetylglucosamine (PNAG). PNAG is a major factor mediating cell-to-cell adhesion in staphylococci (4, 5), and its production depends on genes encoded within the intercellular adhesion (ica) locus (6). The final stage of the biofilm life cycle comprises cell disassembly and subsequent growth in a planktonic mode, a process contributing to S. epidermidis biofilm pathogenesis by disseminating infection (7).S. epidermidis strains with impaired PNAG production display attenuated virulence in foreign body infection models (8, 9), while the production of this polysaccharide conferred a survival advantage on this bacterium infecting the nematode host Caenorhabditis elegans (10). Moreover, ica mutants display growth attenuation when competing in vivo with the corresponding wild-type (WT) strains (11) and commensal ica-negative strains became invasive when transformed with a plasmid containing the ica locus (12). In the clinical setting, the presence of icaA/D genes was associated with therapeutic failure in device-related infections with coagulase-negative staphylococci (13). Despite the observed association of PNAG production with S. epidermidis virulence, it is not entirely clear how this polysaccharide may affect the host immune response. Previous reports have shown that PNAG stimulates the production of proinflammatory mediators (14, 15) but also that it impairs phagocytosis and phagocyte-mediated bacterial killing (16-18). Additionally, little is known about the effects of this polysaccharide on acquired immunity. Here, by using the PNAG-producing S. epidermidis 9142 strain and a PNAG-negative isogenic M10 mutant, we have addressed in the murine model how this polysaccharide affected the host inflammatory response. The results obtained show that mice infected with a PNAG-producing S. epidermidis strain exhibited a more intense inflammatory response than mice infected with an isogenic PNAG-negative mutant. MATERIALS AND METHODSBacteria and growth conditions. Biofilm-forming S. epidermidis strain 9142 (19) and the isogenic PNAG-negative M10 mutant (5) were used. Absence of growth defects in the M10 strain was confirmed, as the two strains had similar generation times, as determined by growing 9142 and M10 bacteria in tryptic soy broth (TSB; Merck, Darmstadt, Germany).

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Specialized immune responses in the peritoneal cavity and omentum

Liu

Silva-Sánchez

Randall

et al. 2020

Journal of Leukocyte Biology

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The peritoneal cavity is a fluid filled space that holds most of the abdominal organs, including the omentum, a visceral adipose tissue that contains milky spots or clusters of leukocytes that are organized similar to those in conventional lymphoid tissues. A unique assortment of leukocytes patrol the peritoneal cavity and migrate in and out of the milky spots, where they encounter Ags or pathogens from the peritoneal fluid and respond accordingly. The principal role of leukocytes in the peritoneal cavity is to preserve tissue homeostasis and secure tissue repair. However, when peritoneal homeostasis is disturbed by inflammation, infection, obesity, or tumor metastasis, specialized fibroblastic stromal cells and mesothelial cells in the omentum regulate the recruitment of peritoneal leukocytes and steer their activation in unique ways. In this review, the types of cells that reside in the peritoneal cavity, the role of the omentum in their maintenance and activation, and how these processes function in response to pathogens and malignancy will be discussed.

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Immune response in the adipose tissue of lean mice infected with the protozoan parasiteNeospora caninum

Cited by 18 publications

References 62 publications

Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

Poly- N -Acetylglucosamine Production by Staphylococcus epidermidis Cells Increases Their In Vivo Proinflammatory Effect

Specialized immune responses in the peritoneal cavity and omentum

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