Immune efficacy of carbon nanotubes recombinant subunit vaccine against largemouth bass ulcerative syndrome virus

Jia, Yijun; Guo, Zi‐Rao; Ma, Rui; Qiu, De-Kui; Zhao, Zhao; Wang, Gao‐Xue; Zhu, Bin

doi:10.1016/j.fsi.2020.03.025

Cited by 31 publications

(15 citation statements)

References 21 publications

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“…Thus, increased serum antibody levels, enzymatic activity, and expression of immune system-related genes (IgM, TGF-β, IL-1β, IL-8, TNF-α, and CD4) were evident in the groups immunized with SWCNTs-MCP compared to the group immunized with MCP alone. 122 Recombinant DNA plasmids combined with CNTs have revealed that immunized fish, show a significant increase in IgM gene expression in relation to the immune system. 123 In one study, Koi herpesvirus (KHV), also called cyprinid herpesvirus 3 (CyHV-3), which affects common carp and ornamental koi carp production, was assessed.…”

Section: Cnts In Viral Treatmentsmentioning

confidence: 99%

Coronavirus and Carbon Nanotubes: Seeking Immunological Relationships to Discover Immunotherapeutic Possibilities

Lima¹,

Octaviano²,

Piqueira³

et al. 2022

IJN

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Since December 2019, the world has faced an unprecedented pandemic crisis due to a new coronavirus disease, coronavirus disease-2019 (COVID-19), which has instigated intensive studies on prevention and treatment possibilities. Here, we investigate the relationships between the immune activation induced by three coronaviruses associated with recent outbreaks, with special attention to SARS-CoV-2, the causative agent of COVID-19, and the immune activation induced by carbon nanotubes (CNTs) to understand the points of convergence in immune induction and modulation. Evidence suggests that CNTs are among the most promising materials for use as immunotherapeutic agents. Therefore, this investigation explores new possibilities of effective immunotherapies for COVID-19. This study aimed to raise interest and knowledge about the use of CNTs as immunotherapeutic agents in coronavirus treatment. Thus, we summarize the most important immunological aspects of various coronavirus infections and describe key advances and challenges in using CNTs as immunotherapeutic agents against viral infections and the activation of the immune response induced by CNTs, which can shed light on the immunotherapeutic possibilities of CNTs.

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Section: Cnts In Viral Treatmentsmentioning

confidence: 99%

Coronavirus and Carbon Nanotubes: Seeking Immunological Relationships to Discover Immunotherapeutic Possibilities

Lima¹,

Octaviano²,

Piqueira³

et al. 2022

IJN

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“…SWCNTs comprise a single graphene cylinder, while MWCNTs comprise multiple coaxial cylinders [159]. Recently, SWCNTs have been extensively studied as carriers to harbor subunit components for the development of fish vaccines [160][161][162][163][164]. Jia et al conjugated a recombinant protein antigen to oxidized SWCNTs (1-2 nm in outside diameter and 0.4-3 µm in length) via carbodiimide chemistry [164].…”

Section: Protein-inorganic Substance Nanoconjugatesmentioning

confidence: 99%

“…Recently, SWCNTs have been extensively studied as carriers to harbor subunit components for the development of fish vaccines [160][161][162][163][164]. Jia et al conjugated a recombinant protein antigen to oxidized SWCNTs (1-2 nm in outside diameter and 0.4-3 µm in length) via carbodiimide chemistry [164]. The resulting conjugates elicited significantly higher antigen-specific antibody titers in fish serum compared to antigens alone, increasing immune protection by 53-80%.…”

Section: Protein-inorganic Substance Nanoconjugatesmentioning

confidence: 99%

Chemical Conjugation Strategies for the Development of Protein-Based Subunit Nanovaccines

Duong

Shalash

et al. 2021

Vaccines

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The production of subunit nanovaccines relies heavily on the development of a vaccine delivery system that is safe and efficient at delivering antigens to the target site. Nanoparticles have been extensively investigated for vaccine delivery over the years, as they often possess self-adjuvanting properties. The conjugation of antigens to nanoparticles by covalent bonds ensures co-delivery of these components to the same subset of immune cells in order to trigger the desired immune responses. Herein, we review covalent conjugation strategies for grafting protein or peptide antigens onto other molecules or nanoparticles to obtain subunit nanovaccines. We also discuss the advantages of chemical conjugation in developing these vaccines.

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“…China and the world at large. How prevention and therapy the disease has been a tremendous topic for researchers from all over the world (Embregts et al, 2017(Embregts et al, , 2019Jia, Guo, et al, 2020;Li et al, 2018;Shen et al, 2018;. Regrettably, until today, there is neither a commercial vaccine for the control and prevention nor a specific medicine for the treatment of SVCV.…”

Section: Svcv Has Aroused Widespread Interest Among Researchers Inmentioning

confidence: 99%

“…The filtered mixture was washed three times with PBS (pH 7.4) to obtain mannosylated G protein (MG). Then, according to our previous research, MG and functionalized single-walled carbon nanotubes (SWCNTs) are coupled to form SWCNTs-MG (Guo et al, 2020;Jia, Guo, et al, 2020;Zhang et al, 2020). The well-mixed SWCNTs-MG was placed in a freeze dryer and lyophilized into powder, and stored at 4℃ for later use.…”

Section: Preparation Of Swcnts-mg Vaccinementioning

confidence: 99%

Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

Gong

Zhang

et al. 2021

Journal of Fish Diseases

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Immersion vaccination of single-walled carbon nanotubes loaded with mannosemodified glycoprotein (SWCNTs-MG) vaccine has been proved to be effective in preventing spring viraemia of carp virus (SVCV). Immunization procedure has immense consequence on the immune effect of the immersion vaccine. However, immunization procedure optimization for SWCNTs-MG vaccine against SVCV has not been reported. In this study, accordingly, a full-factor experiment was designed to optimize the immunization procedure of SWCNTs-MG vaccine by three aspects of vaccine dose (30 mg/L, 40 mg/L and 50 mg/L), immunization density (8 fish L −1 , 24 fish L −1 and 48 fish L −1 ) and immunization time (6, 12 and 24 hr). Furthermore, we used the immunization group (A1B2C1, 30 mg/L, 24 fish L −1 and 6 hr) in the previous study as a positive control (PC) to evaluate the immunization effect optimized conditions from the expression of immune-related genes and relative percentage survival (RPS). At 28 days post-vaccination (DPV), common carps were intraperitoneal injected SVCV challenged test indicated that the A1B2C2 group (30 mg/L, 24 fish L −1 , 12 hr) displayed superiority of protective efficacy compare with other groups and the RPS with 77.9%, which was 15.6% higher than the PC group of RPS with 62.3%.Moreover, the expression of immune-related genes such as IL-10, CD4 and MHC-II was also significantly higher than PC group. The specific experimental flow chart is shown in Figure 1. Conclusively, these results demonstrated that vaccine dose, immunization density and immunization time are 30 mg/L, 24 fish L −1 and 12 hr, which is the more appropriate immunization programme with juvenile carp for SWCNTs-MG vaccine. This study provides a profitable reference for improving the immune efficiency of aquatic immersion vaccine.

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Immune efficacy of carbon nanotubes recombinant subunit vaccine against largemouth bass ulcerative syndrome virus

Cited by 31 publications

References 21 publications

Coronavirus and Carbon Nanotubes: Seeking Immunological Relationships to Discover Immunotherapeutic Possibilities

Coronavirus and Carbon Nanotubes: Seeking Immunological Relationships to Discover Immunotherapeutic Possibilities

Chemical Conjugation Strategies for the Development of Protein-Based Subunit Nanovaccines

Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

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