Immune cell infiltration of normal and impaired sow endometrium

Dalin, Anne-Marie; Kaeoket, Kampon; Persson, Erik

doi:10.1016/j.anireprosci.2004.04.012

Cited by 31 publications

(54 citation statements)

References 43 publications

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“…On the contrary, if the pigs were under an influence of estradiol, the function of immune cells would be upregulated [29]. Specifically, the preceding studies revealed that the correlation between serum P 4 concentration and the number of eosinophils was found [5,22]. Likewise, the tendency of positive correlation between serum P 4 concentration and the infiltration of eosinophils in the current study was observed.…”

Section: Discussionsupporting

confidence: 54%

“…Histological examination: Endometrial sections were classified as "acute endometritis," "chronic endometritis," and "normal endometrium" according to both gross morphological findings and histological examination from hematoxylin and eosin (H&E) stained sections [5,26]. Moreover, immunocytes, including neutrophils, lymphocytes, eosinophils, macrophages, and plasma cells in different layers (surface epithelial, subepithelial connective tissue, and glandular connective tissue layers) were quantified under light microscope (400 ) in 20 randomly microscopic fields per endometrial layer [15,24].…”

Section: Tissue Collectionmentioning

confidence: 99%

“…However, an elucidation of endometritis on the criterion of histological examination is relatively complicated since estrous cycle and/or insemination can be the factors predominating on the infiltration of immune cells into the endometrium [5,15,24]. Earlier studies have demonstrated that an increase in serum progesterone (P 4 ) concentration contributes to a decrease in the number of local immune cells infiltrating into the endometrium [24,29].…”

mentioning

confidence: 99%

“…The inflammation degree of the endometrium can be classified by type and number of local immune cells infiltrating into the endometrium [5,26]. However, an elucidation of endometritis on the criterion of histological examination is relatively complicated since estrous cycle and/or insemination can be the factors predominating on the infiltration of immune cells into the endometrium [5,15,24].…”

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confidence: 99%

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Expression of Cyclooxygenase-2 in the Endometrium of Gilts with Different Stages of Endometritis

et al. 2011

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ABSTRACT. The present study determined the association among the expression of COX-2, stages of endometritis, and types and number of local immune cells infiltrating into the gilts' endometrium. The uterine tissues from 24 Landrace x Yorkshire gilts identified as acute endometritis (n=7), chronic endometritis (n=7), and normal endometrium (n=10) were included. The tissues were prepared for both histological and immunohistochemical investigations. The immunoexpression of COX-2 in every layer of the gilts' endometria was appraised by avidin-biotin-peroxidase complex method via image analysis; and was reported as percentage of positive area and staining index. The results revealed that the immunoexpression of COX-2 was found only in the surface epithelial layer. The gilts with acute endometritis possessed higher both percentage of positive area (68.99% versus 4.50% and 3.43%, P<0.001) and staining index (1.13 versus 0.05 and 0.04, P<0.001) than those with chronic endometritis and normal endometrium, respectively. Positive correlations between the number of surface epithelial neutrophils and percentage of COX-2 positive area (r=0.47, P=0.022), as well as mean staining index (r=0.44, P=0.032) were observed. In conclusion, the immunoexpression of COX-2 was found strongest in the gilts with acute endometritis, meanwhile it was not different between those with chronic endometritis and normal endometrium. This suggested that the expression of COX-2 might be dependent not only on the infiltration of local immune cells in the endometrium, but also on the duration of exposure with inflammatory agents.

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Section: Discussionsupporting

confidence: 54%

Section: Tissue Collectionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Expression of Cyclooxygenase-2 in the Endometrium of Gilts with Different Stages of Endometritis

et al. 2011

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“…After insemination, excessive sperm are eliminated by the local immune cells of the sow endometrium [2,8]. These immune cells are produced from either lymphoid (e.g., lymphocyte, plasma cell) or myeloid lineage (e.g., neutrophil, macrophage and eosinophil) in the bone marrow [2].…”

mentioning

confidence: 99%

Infiltration of Local Immune Cells in the Sow Reproductive Tracts after Intra-Uterine and Deep Intra-Uterine Insemination with a Reduced Number of Spermatozoa is Less than Conventional Artificial Insemination

Tummaruk

Tienthai

2011

The Journal of Veterinary Medical Science

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ABSTRACT. The present study investigated the infiltration of leukocyte subpopulations in the utero-tubal junction (UTJ) and each part of the oviducts at about 24 hr after intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI) compared to conventional artificial insemination (CAI) in sows. Fifteen crossbred Landrace x Yorkshire multiparous sows were used (CAI, n=5; IUI, n=5; DIUI, n=5). The sperm dose contained 3,000  10 6 (100 ml), 1,000  10 6 (50 ml) and 150  10 6 (5 ml) motile spermatozoa for CAI, IUI and DIUI, respectively. The sows were inseminated with extended fresh semen at 6 to 8 hr prior to the expected time of ovulation. At 25.2  1.6 hr after insemination, the oviducts and the UTJ were collected. The tissue samples of UTJ, caudal isthmus, cranial isthmus and ampulla were transversely cut to a thickness of 5 m and stained with H&E. The total numbers of lymphocytes, neutrophils, macrophages, eosinophils and plasma cells were determined under light microscope. It was found that the numbers of lymphocytes, eosinophils and macrophages after CAI, IUI and DIUI were not significantly different (P>0.1) in both epithelial and sub-epithelial connective tissue layer of the UTJ, caudal isthmus, cranial isthmus and ampulla. Intra-epithelial neutrophils in the UTJ were higher than cranial isthmus (P<0.05) and ampulla (P<0.05). In the UTJ, the intra-epithelial neutrophil in the CAI group was higher than DIUI group (P<0.01). Plasma cells in sub-epithelial layer of the endosalpinx in the CAI group were higher than DIUI group (P<0.05) and tended to be higher than the IUI group (P=0.08). In conclusion, compared to CAI, IUI and DIUI do not influence the infiltration of lymphocytes, macrophages and eosinophils in the UTJ and the oviduct prior to fertilization. But a lower number of neutrophils in the intra-epithelial layer of the UTJ and plasma cells in the sub-epithelial layers of the oviduct was observed in the DIUI group compared to CAI.KEY WORDS: immune cell, intra-uterine insemination, oviduct, swine.

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