Immobilization onto gold nanoparticles alters α-lactalbumin interaction with pure and mixed phospholipid monolayers

Lystvet, Sina M.; Volden, Sondre; Halskau, Øyvind; Glomm, Wilhelm R.

doi:10.1039/c1sm06337e

Cited by 16 publications

(31 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to confirm this, we performed a controlled experiment in which urease solution was incubated with ANS and citrate capped Au nanoparticles (maintaining the HAuCl 4 concentration same as used in the urease reduction). The fluorescence results (Figure 3b) showed that the emission intensity of ANS was indeed quenched by the Au nanoparticles with a change in line-shape signifying the energy transfer40. However the quenching was not as much as observed for the urease reduced Au nanoparticles, suggesting that both energy transfer as well as partial unfolding of urease contributed to the emission quenching of ANS.…”

Section: Resultsmentioning

confidence: 92%

See 1 more Smart Citation

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Sharma

Mandani

Sarma

2013

Sci Rep

View full text Add to dashboard Cite

Biomineralization is an extremely efficient biologically guided process towards the advancement of nano-bio integrated materials. As a prime module of the natural world, enzymes are expected to play a major role in biogenic growth of inorganic nanostructures. Although there have been developments in designing enzyme-responsive nanoparticle systems or generation of inorganic nanostructures in an enzyme-stimulated environment, reports regarding action of enzymes as reducing agents themselves for the growth of inorganic nanoparticles still remains elusive. Here we present a mechanistic investigation towards the synthesis of metal and metallic alloy nanoparticles using a commonly investigated enzyme, Jack bean urease (JBU), as a reducing as well as stabilizing agent under physiological conditions. The catalytic functionality of urease was taken advantage of towards the development of metal-ZnO core-shell nanocomposites, making urease an ideal bionanoreactor for synthesizing higher order nanostructures such as alloys and core- shell under ambient conditions.

show abstract

Section: Resultsmentioning

confidence: 92%

“…The conformational studies were performed by fluorescence spectroscopy using 1-anilino-8-naphthalene sulfonate (ANS) as an extrinsic probe, as reported earlier for protein studies40. ANS is highly fluorescent in hydrophobic environment such as interior of an enzyme, but has very low quantum yield in water.…”

Section: Resultsmentioning

confidence: 99%

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Sharma

Mandani

Sarma

2013

Sci Rep

View full text Add to dashboard Cite

show abstract

“…15,18,19 The change in surface pressure upon injecting Tf and Tf-AuINT under an air/water interface (phosphate buffer, pH 7.4) is shown in Figure 4a. 15,18,19 The change in surface pressure upon injecting Tf and Tf-AuINT under an air/water interface (phosphate buffer, pH 7.4) is shown in Figure 4a.…”

Section: Interaction With Lipid Monolayersmentioning

confidence: 99%

“…[15][16][17][18][19] The average changes in trough area upon injection of Tf and Tf-AuINT under a precompressed phospholipid film are shown in Figure 4b. Consequently, only Tf and Tf-AuINT results are shown here.…”

Section: Interaction With Lipid Monolayersmentioning

confidence: 99%

Self-assembly and characterization of transferrin–gold nanoconstructs and their interaction with bio-interfaces

et al. 2015

Self Cite

View full text Add to dashboard Cite

Transferrin (Tf) conjugated to gold nanoparticles and clusters combine the protein's site-specific receptor targeting capabilities with the optical properties imparted by the nano-sized gold. We have described two different synthesis protocols, one yielding fluorescent Tf-stabilized gold nanoclusters (AuNCs) and one yielding Tf-stabilized gold nanoparticles that exhibit localized surface plasmon resonance. We demonstrate that the synthetic route employed has a large influence both on the gold nanostructure formed, and also on the structural integrity of the protein. A slight protein unfolding allows stronger interaction with lipids, and was found to significantly perturb lipid monolayers. Interactions between the protein-gold nanostructures and three different cell types were also assessed, indicating that the enhanced membrane affinity may be attributed to intercellular membrane differences.

show abstract

“…As it is known, bio-macromolecules are endowed with complex and deformable architectures, with many functional groups available to binding. Only certain conformations permit globular proteins an extensive and homogeneous adsorption onto nanoparticles [30]. Sometimes, the biopolymers are properly modified for interactions with CNTs to be significant, to ensure effective stabilization.…”

mentioning

confidence: 99%

Dispersability of Carbon Nanotubes in Biopolymer-Based Fluids

Tardani

Mesa

2015

Crystals

View full text Add to dashboard Cite

Abstract:In this review the dispersability of carbon nanotubes in aqueous solutions containing proteins, or nucleic acids, is discussed. Data reported previously are complemented by unpublished ones. In the mentioned nanotube-based systems several different phases are observed, depending on the type and concentration of biopolymer, as well as the amount of dispersed nanotubes. The phase behavior depends on how much biopolymers are adsorbing, and, naturally, on the molecular details of the adsorbents. Proper modulation of nanotube/biopolymer interactions helps switching between repulsive and attractive regimes. Dispersion or phase separation take place, respectively, and the formation of liquid crystalline phases or gels may prevail with respect to dispersions. We report on systems containing ss-DNA-and lysozyme-stabilized nanotubes, representative of different organization modes. In the former case, ss-DNA rolls around CNTs and ensures complete coverage. Conversely, proteins randomly and non-cooperatively adsorb onto nanotubes. The two functionalization mechanisms are significantly different. A fine-tuning of temperature, added polymer, pH, and/or ionic strength conditions induces the formation of a given supra-molecular organization mode. The biopolymer physico-chemical properties are relevant to induce the formation of different phases made of carbon nanotubes.

show abstract

Immobilization onto gold nanoparticles alters α-lactalbumin interaction with pure and mixed phospholipid monolayers

Cited by 16 publications

References 51 publications

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Self-assembly and characterization of transferrin–gold nanoconstructs and their interaction with bio-interfaces

Dispersability of Carbon Nanotubes in Biopolymer-Based Fluids

Contact Info

Product

Resources

About