Self-assembly and characterization of transferrin–gold nanoconstructs and their interaction with bio-interfaces

McDonagh, Birgitte Hjelmeland; Volden, Sondre; Lystvet, Sina M.; Singh, Gurvinder; Ese, Marit-Helen Glomm; Ryan, Joseph A.; Lindgren, Mikael; Sandvig, Axel; Sandvig, Ioanna; Glomm, Wilhelm R.

doi:10.1039/c5nr01284h

Cited by 12 publications

(6 citation statements)

References 32 publications

(106 reference statements)

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“…Positively charged NSs have a strong protein adsorption in biological media and can severely damage the membranes of cells. Due to this, a neutral or negative charge is preferable to avoid strong adsorption on cell membranes and/or protein adsorption [ 26 , 60 ]. Furthermore, the shape of NSs will determine to which extent the surface coverage of stabilizing molecules is uniform or not [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

Growing gold nanostructures for shape-selective cellular uptake

et al. 2018

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With development in the synthesis of shape- and size-dependent gold (Au) nanostructures (NSs) and their applications in nanomedicine, one of the biggest challenges is to understand the interaction of these shapes with cancer cells. Herein, we study the interaction of Au NSs of five different shapes with glioblastoma-astrocytoma cells. Three different shapes (nanorods, tetrahexahedra, and bipyramids), possessing tunable optical properties, have been synthesized by a single-step seed-mediated growth approach employing binary surfactant mixtures of CTAB and a secondary surfactant. By the use of two-step seed-mediated approach, we obtained new NSs, named nanomakura (Makura is a Japanese word used for pillow) which is reported for the first time here. Spherical Au nanoparticles were prepared by the Turkevich method. To study NS-cell interactions, we functionalized the NSs using thiolated PEG followed by 11-Mercaptoundecanoic acid. The influence of shape and concentration of NSs on the cytotoxicity were assessed with a LIVE/DEAD assay in glioblastoma-astrocytoma cells. Furthermore, the time-dependent uptake of nanomakura was studied with TEM. Our results indicate that unlike the other shapes studied here, the nanomakura were taken up both via receptor-mediated endocytosis and macropinocytosis. Thus, from our library of different NSs with similar surface functionality, the shape is found to be an important parameter for cellular uptake.Electronic supplementary materialThe online version of this article (10.1186/s11671-018-2662-7) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Growing gold nanostructures for shape-selective cellular uptake

et al. 2018

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“…As proteins are nanoscale biomacromolecules with unique three-dimensional structures, protein-templated Au NCs showed great potential for biomedical applications in a variety of biological conditions . Many protein molecules, such as transferrin, bovine serum albumin (BSA), and insulin, have been explored to develop fluorescent Au NCs for biosensing and imaging applications. , …”

Section: Introductionmentioning

confidence: 99%

“…13 Many protein molecules, such as transferrin, bovine serum albumin (BSA), and insulin, have been explored to develop fluorescent Au NCs for biosensing and imaging applications. 14,15 However, protein-templated Au NCs are not ready to be used for molecular receptor-specific imaging in biomedical applications. To achieve active targeting, Au NCs have been conjugated with site-specific ligands, such as aptamer, peptide, and antibody, which serve as guiding moieties to recognize tumor-related targets specifically.…”

Section: ■ Introductionmentioning

confidence: 99%

Monoclonal-Antibody-Templated Gold Nanoclusters for HER2 Receptors Targeted Fluorescence Imaging

Zhang

Chen

Zhang

et al. 2020

ACS Appl. Bio Mater.

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HER2 receptor-specific monoclonal-antibody-templated gold nanoclusters, Herceptin-templated Au NCs (Her-Au NCs), have been successfully obtained via “green” synthesis. This strategy allows the fluorescent gold nanoclusters (Au NCs) formed in the three-dimensional structure of Herceptin without destroying the high specificity and affinity to HER2 receptors. The Her-Au NCs have been found to be superior compared to Cy3-Herceptin in the fluorescence emission (λem = 645 nm) and the photostability under high-intensity UV irradiation or long-time storage. Moreover, Her-Au NCs can achieve receptor-specific imaging without targeted modification owing to the HER2-binding ability of the Herceptin scaffold. For imaging applications, Her-Au NCs can be utilized as effective optical probes for not only fluorescence imaging of HER2-positive cancer cells but also imaging of HER2-positive tumors in vivo.

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“…It has been demonstrated that chitosan can be used as stabilizer and as reducing agent to synthesize gold nanoparticles, however, longer times of reaction and heating about 45–95 °C is needed, moreover, the nanoparticles synthesized do not have control in shape and size . It is very attractive to integrate nanoparticles with biological molecules such as DNA, and proteins, aminoacids, polysaccharides, and vitamins to create new materials for potential new applications in electronics, optics, genomics, proteomics, and biomedical and bioanalytical areas . However, the use of semisynthetic derivatives of these molecules could enhance the interaction between nanoparticles and the targeted molecules without loss of biocompatibility and bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Polyelectrolytes with sulfonate groups obtained by chemical modification of chitosan useful in green synthesis of Au and Ag nanoparticles

Caldera‐Villalobos

Garcı́a-Serrano

Peláez-Cid

et al. 2017

J of Applied Polymer Sci

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Usually the metal nanoparticles are obtained by different chemical reactions that are not environmentally friendly. This paper describes the synthesis of two polyelectrolytes with sulfonate groups in ortho-position and in ortho-and para-positions, which were obtained by chemical modification of chitosan. They were used in the green synthesis of Au and Ag nanoparticles by colloidal method in aqueous solution. Polyelectrolytes were used as reducing agents of Au 31 and Ag 1 ions and as stabilizing agents of Ag and Au nanoparticles. The hydroxyl and imine groups in the polyelectrolytes are reducing agents of Au 31 and Ag 1 ions while the sulfonate groups and the polymer backbone stabilized Au and Ag nanoparticles. Polyelectrolyte 1, which has sulfonate group in ortho-position, favors the obtaining of anisotropic Au nanoparticles with an average size of 19 nm. While the polyelectrolyte 2, with two sulfonate groups in the ortho-and para-positions, yielded quasi-spherical Au nanoparticles with an average size of 14 nm. In general, Ag nanoparticles stabilized with both polyelectrolytes, show quasi-spherical forms with good control in size. Finally, both polyelectrolytes have the ability to protect the Au and Ag nanoparticles allowing obtaining colloidal solutions that are stable for several months.

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Self-assembly and characterization of transferrin–gold nanoconstructs and their interaction with bio-interfaces

Cited by 12 publications

References 32 publications

Growing gold nanostructures for shape-selective cellular uptake

Growing gold nanostructures for shape-selective cellular uptake

Monoclonal-Antibody-Templated Gold Nanoclusters for HER2 Receptors Targeted Fluorescence Imaging

Polyelectrolytes with sulfonate groups obtained by chemical modification of chitosan useful in green synthesis of Au and Ag nanoparticles

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