Immobilization of nitrifying bacteria on composite based on polymers and eggshells for nitrate production

Chaali, Mona; Ortiz, Hugo Alejandro Rivera; Cano, Beatriz Delgado; Brar, Satinder Kaur; Ramírez, Antonio Avalos; Arriaga, Sonia; Heitz, Michèle

doi:10.1016/j.jbiosc.2021.01.010

Cited by 6 publications

(6 citation statements)

References 49 publications

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“…Interestingly, addition of EP facilitates the metabolic activity of bacterial cells, which is consistent with the previous study. 29 Overall, the metabolic activity results agree with the plate-counting method, indicating the enhanced effectiveness of AgNPs@EP to inactivate biofilms on the plastic surface. SEM imaging was acquired to validate the biofilm removal by AgNPs@EP treatment for 2 h. The images in Figure 6c and d demonstrate that E. coli and L. innocua biofilms (consisting of EPS and bacterial cells) were extensively formed on the plastic surface before treatment.…”

Section: Enhanced Storage Stability Of Agnps@ep the Results In Figuresupporting

confidence: 67%

“…For the two control groups (PBS- and EP-treated biofilms), the fluorescent signals remained high and detectable during the 12-h period, suggesting live bacterial cells. Interestingly, addition of EP facilitates the metabolic activity of bacterial cells, which is consistent with the previous study . Overall, the metabolic activity results agree with the plate-counting method, indicating the enhanced effectiveness of AgNPs@EP to inactivate biofilms on the plastic surface.…”

Section: Resultsmentioning

confidence: 94%

“…Such high binding affinity between EP and the biofilm matrices is potentially due to (a) surface hydrophobicity of EP and (b) surface roughness and porosity of EP. First, the hydrophobic surface of EP promotes the van der Waals interaction with bacterial cells. , Second, the porosity of EP creates surface roughness that exposes a larger surface area for bacterial adhesion on EP . In addition, surface roughness of EP is further induced by the dehydration ability of the abundant proteins within EP such that more area is revealed to interact with the “protruding parts” of bacteria .…”

Section: Discussionmentioning

confidence: 99%

“…First, the hydrophobic surface of EP promotes the van der Waals interaction with bacterial cells. , Second, the porosity of EP creates surface roughness that exposes a larger surface area for bacterial adhesion on EP . In addition, surface roughness of EP is further induced by the dehydration ability of the abundant proteins within EP such that more area is revealed to interact with the “protruding parts” of bacteria . Therefore, the unique surface properties of EP lead to its high affinity to bind biofilms.…”

Section: Discussionmentioning

confidence: 99%

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Biobased Chicken Eggshell Powder for Efficient Delivery of Low-Dose Silver Nanoparticles (AgNPs) to Enhance Their Antimicrobial Activities against Foodborne Pathogens and Biofilms

Tao

Huang

2022

ACS Appl. Bio Mater.

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Despite the current sanitation practices to decontaminate food-contact surfaces, persistent biofilms still pose significant threats to human health by inducing cross-contamination. This study aims to enhance the antimicrobial activity of low-dose silver nanoparticles (AgNPs) against foodborne pathogens and their biofilms through the development of a biobased delivery carrier for metallic nanoparticles. In this study, chicken eggshell powder (EP) was used as a biocompatible delivery carrier, and it possesses a strong ability to encapsulate green-synthesized AgNPs with an encapsulation efficiency of 80.18%. The EP carriers stabilized AgNPs in an organic-rich environment and prevented the aggregation of nanoparticles. The results of antimicrobial test demonstrate that EP significantly enhanced the antimicrobial efficacy of low-dose AgNPs (2 μg/mL), enabling 5-log reductions of planktonic Escherichia coli and Listeria innocua within 25 min and 60 min treatments, respectively, even in the presence of high organic content (chemical oxygen demand, COD = 1000 mg/L). Due to the high affinity of EP to bind biofilms, the encapsulated low-dose AgNPs can inactivate approximately 2-log CFU/cm 2 of biofilms within a 2-h treatment. The proposed AgNPs@EP composite with a low silver concentration (2 μg/mL) can effectively inactivate and remove biofilms from food-contact surfaces in which such a low concentration of AgNPs is unlikely to induce negative impacts on human health and environment. Therefore, this antimicrobial AgNPs@EP composite can potentially be used as a biobased sanitizer for food-contact surfaces in a food manufacturing plant.

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Section: Enhanced Storage Stability Of Agnps@ep the Results In Figuresupporting

confidence: 67%

Section: Resultsmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Biobased Chicken Eggshell Powder for Efficient Delivery of Low-Dose Silver Nanoparticles (AgNPs) to Enhance Their Antimicrobial Activities against Foodborne Pathogens and Biofilms

Tao

Huang

2022

ACS Appl. Bio Mater.

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“…Recent carriers used for enrichment have included activated carbon, ceramic, composites, etc. (Chaali et al, 2021;Parker et al, 2002;Li et al, 2013;Han & Park, 2012). After enrichment, the nitrifying carrier were also widely used for improving sustainable nitrification in lab-scale and full-scale (Wang et al, 2013;Liu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Construction of Nitrification Model With Nitrifying Coal Ash in Aerobic Treatment of High Strength Wastewater

Liu

Zhao

Pan

et al. 2022

Journal of Environmental Engineering and Landscape Management

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Nitrifying carriers can provide good settle ability and stable removal efficiency for nitrogen. Models for ammonia removal rate for nitrifying carriers will improve its engineering application. This study was conducted in nitrifying coal ash system with Monod model. Results indicated the maximum NH4+-N removal rate and half-saturation constant of NH4+-N in Monod model were 110.48 mg/L and 59.19 mg/L, respectively. Introduction of the correction coefficients, including pH, temperature and dissolved oxygen (DO) concentration, decreased the average gap between experiment data and simulated data from 6.48 to 2.74 mg N/(L·h). And improved accuracy of the Monod model by 5.11%. The differences between experiment and simulated NH4+-N removal rate ranged from 0.08 mg N/(L·h) to 8.34 mg N/(L·h) when the influent concentration of NH4+-N increased from 443.18 to 1121.29 mg N/L and without organic. Only 0.08% inconsistency between experiment and simulated data occurred in treating wastewater with high-strength ammonia. However, NH4+-N removal rate of the nitrifying coal ash was inhibited about 40% when influent with averaged 173.19 mg COD/L and 37.20 mg N/L, therefore, other factors, the content of nitrifying bacteria for example, need to be introduced into the Monod model when treating organic wastewater.

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Biosorption of cadmium(II) from aqueous solution by immobilized Bacillus cereus on eggshell powder

Rasheed,

Jawad,

Ghous

et al. 2023

Biomass Conv. Bioref.

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Immobilization of nitrifying bacteria on composite based on polymers and eggshells for nitrate production

Cited by 6 publications

References 49 publications

Biobased Chicken Eggshell Powder for Efficient Delivery of Low-Dose Silver Nanoparticles (AgNPs) to Enhance Their Antimicrobial Activities against Foodborne Pathogens and Biofilms

Biobased Chicken Eggshell Powder for Efficient Delivery of Low-Dose Silver Nanoparticles (AgNPs) to Enhance Their Antimicrobial Activities against Foodborne Pathogens and Biofilms

Construction of Nitrification Model With Nitrifying Coal Ash in Aerobic Treatment of High Strength Wastewater

Biosorption of cadmium(II) from aqueous solution by immobilized Bacillus cereus on eggshell powder

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