Biobased Chicken Eggshell Powder for Efficient Delivery of Low-Dose Silver Nanoparticles (AgNPs) to Enhance Their Antimicrobial Activities against Foodborne Pathogens and Biofilms

Abstract

Despite the current sanitation practices to decontaminate food-contact surfaces, persistent biofilms still pose significant threats to human health by inducing cross-contamination. This study aims to enhance the antimicrobial activity of low-dose silver nanoparticles (AgNPs) against foodborne pathogens and their biofilms through the development of a biobased delivery carrier for metallic nanoparticles. In this study, chicken eggshell powder (EP) was used as a biocompatible delivery carrier, and it possesses a strong ability to encapsulate green-synthesized AgNPs with an encapsulation efficiency of 80.18%. The EP carriers stabilized AgNPs in an organic-rich environment and prevented the aggregation of nanoparticles. The results of antimicrobial test demonstrate that EP significantly enhanced the antimicrobial efficacy of low-dose AgNPs (2 μg/mL), enabling 5-log reductions of planktonic Escherichia coli and Listeria innocua within 25 min and 60 min treatments, respectively, even in the presence of high organic content (chemical oxygen demand, COD = 1000 mg/L). Due to the high affinity of EP to bind biofilms, the encapsulated low-dose AgNPs can inactivate approximately 2-log CFU/cm 2 of biofilms within a 2-h treatment. The proposed AgNPs@EP composite with a low silver concentration (2 μg/mL) can effectively inactivate and remove biofilms from food-contact surfaces in which such a low concentration of AgNPs is unlikely to induce negative impacts on human health and environment. Therefore, this antimicrobial AgNPs@EP composite can potentially be used as a biobased sanitizer for food-contact surfaces in a food manufacturing plant.