Immobilization of Lactate Dehydrogenase on Electrochemically Prepared Polyaniline Films

Abstract

Immobilization of lactate dehydrogenase (LDH) has been carried out on electrochemically prepared polyaniline (PANI) ®lms. The results of the photometric and amperometric response measurements conducted on such LDHaPANI electrodes show the response time as 90 s and shelf-life as about two weeks at 4±10 C indicating that these conducting polymer-enzyme electrodes can be used as a pyruvate sensor.

A self assembled monolayer based microfluidic sensor for urea detection

“…11,12 The modification of free terminal groups of thiolated SAMs with biomolecules using physical adsorption may result in denaturation and loss of activity of the desired biomolecules. However, binding of a biomolecule using a chemical activator arising due to the resultant covalent bond may lead to increased stability of a biosensor.…”

α-MoO3 nanowire-based amperometric biosensor for l-lactate detection

“…The results indicate that the oxidation current density increases significantly with increasing pH, reaching a maximum at pH 7. Above pH 7, we observed a decrease in enzyme activity, which is in good agreement with results obtained for other previously reported electrochemical sensors [20][21][22].…”

Electrocatalytic reduction of hydrogen peroxide performed by poly(pyrrole) / Prussian blue composites

“…This extended π-conjugated system leads to its intrinsic properties, such as high electrical conductivity, low energy optical transitions, low ionization potential and high electron affinity [3]. All these interesting features allow the use of conducting polymers in different fields of technology such as solar cells, light weight batteries, electrochromic devices, sensors, molecular electronic devices supercapacitors for energy storage and secondary batteries [2][3][4].…”