Immobilization of horseradish peroxidase via an amino silane on oxidized ultrananocrystalline diamond

Diamond‐based DNA sensors: surface functionalization and read‐out strategies

“…The diamond surface has been functionalized successfully with various biomolecules: not only with DNA as was already discussed, but also with immunoglobulins G and M (IgG and IgM) [70], and enzymes such as catalase [71], urease, glucose, oxidase [72], and horse radish peroxidase [73].…”

Nanoindentation as a Tool to Clarify the Mechanism Causing Variable Stiffness of a Silane Layer on Diamond

“…Diamond may be more biocompatible for direct protein immobilization than, for example, metal surfaces [22]. However, from our experience of single-molecule dynamics [23,24], as well as that of others' [10,[25][26][27][28][29][30], we still recommend the use of an intervening layer for preserving enzymatic activities. Polymer layers have a distinct advantage compared with flat solid surfaces in terms of retaining protein activity.…”

“…A wide range of various silanes, such as aminosilanes (-NH2), mercaptosilanes (-SH), and glycidoxytrimethoxysilanes (epoxide), is commercially available, allowing different chemical functionalities to be incorporated on the surfaces [34]. Formation of aminosilane layers on a diamond surface has been reported in several studies [10,26,27]. In our work, we used 3-aminopropyltriethoxysilane (APTES), which is one of the commonly used aminosilanes.…”

Silylation of clay mineral surfaces

“…Recently, great efforts have been made to silylate kaolinite interlayer surfaces, and the introduced functional groups via silylation are capable of enduing the silylated products with versatile properties, such as organophilicity (Gârea et al, 2008), metal affinity (Tan et al, 2011) and enzyme loading (Hernando et al, 2007;Kim et al, 2006;Kobayashi and Matsunaga, 1991). A breakthrough about preparation of silane grafted kaolinite was achieved by Tonlé et al (2007).…”