Immobilization of Glycoside Hydrolase Families GH1, GH13, and GH70: State of the Art and Perspectives

Graebin, Natália G.; Schöffer, Jéssie da Natividade; Andrades, Diandra de; Hertz, Plinho Francisco; Ayub, Marco Antônio Záchia; Rodrigues, Rafael C.

doi:10.3390/molecules21081074

Cited by 49 publications

(24 citation statements)

References 300 publications

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“…For example, endoglucanases (EC 3.2.1.4) from Bacillus that have cellulose degradation function usually belonged to the GH5 families [29] ( Table 2). In the genome, six GH13s were obliged to hydrolyze starch, such as α-amylase, α-glucosidase and α-glycosidase [30]. Four GH4s, three GH1s and one GH16 exhibited potential cellulose degradation because of enzyme function.…”

Section: Carbohydrate-active Enzyme (Cazyme) Annotationmentioning

confidence: 99%

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Lü

Zheng

et al. 2020

Biotechnol Biofuels

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Background: Bamboo, a lignocellulosic feedstock, is considered as a potentially excellent raw material and evaluated for lignocellulose degradation and bioethanol production, with a focus on using physical and chemical pre-treatment. However, studies reporting the biodegradation of bamboo lignocellulose using microbes such as bacteria and fungi are scarce. Results:In the present study, Bacillus velezensis LC1 was isolated from Cyrtotrachelus buqueti, in which the symbiotic bacteria exhibited lignocellulose degradation ability and cellulase activities. We performed genome sequencing of B. velezensis LC1, which has a 3929,782-bp ring chromosome and 46.5% GC content. The total gene length was 3,502,596 bp using gene prediction, and the GC contents were 47.29% and 40.04% in the gene and intergene regions, respectively. The genome contains 4018 coding DNA sequences, and all have been assigned predicted functions. Carbohydrate-active enzyme annotation identified 136 genes annotated to CAZy families, including GH, GTs, CEs, PLs, AAs and CBMs. Genes involved in lignocellulose degradation were identified. After a 6-day treatment, the bamboo shoot cellulose degradation efficiency reached 39.32%, and the hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 7.2 g/L of ethanol at 96 h. Conclusions:These findings provide an insight for B. velezensis strains in converting lignocellulose into ethanol. B. velezensis LC1, a symbiotic bacteria, can potentially degrade bamboo lignocellulose components and further transformation to ethanol, and expand the bamboo lignocellulosic bioethanol production.

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Section: Carbohydrate-active Enzyme (Cazyme) Annotationmentioning

confidence: 99%

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Lü

Zheng

et al. 2020

Biotechnol Biofuels

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“…GH32 contains sucrose-6-phosphate hydrolase and levanase, and are thus expected to function in sucrose hydrolysis [34]. GH13 (α-amylase, α-glucosidase, α-glycosidase, and α-α-phosphorylase) and CBM50 (involved in starch hydrolysis) [35] were also detected. GH65 (maltose phosphorylase) was identified in several B. velezensis genomes; this sequence is associated with trehalose degradation [36].…”

Section: Resultsmentioning

confidence: 99%

Comparative genomic and secretomic characterisation of endophytic Bacillus velezensis LC1 producing bioethanol from bamboo lignocellulose

Tang

Zheng

et al. 2019

Preprint

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Background: Compared to physical and chemical methods, microbial lignocellulosic degradation is a green process. Bacillus is an excellent organic matter degrader, and it has exhibited various abilities required for lignocellulose degradation, including the degradation of cellulose, hemicellulose and lignin. Several B. velezensis strains encode lignocellulosases. However, their usefulness based on their ability to transform biomass has not been appreciated. Genomic, comprehensive comparative genomic and secretomic analyses were used to clarify the lignocellulose-degrading potential of these bacteria, which is necessary for their future application.Results: In the present study, the complete genomes of 20 B. velezensis strains and an endophytic bacterium, B. velezensis LC1, were analysed to find common and unique genes encoding carbohydrate-active enzymes (CAZymes) and evaluate their potentials to degrade lignocellulose. By comparative whole genomic and CAZyomic analyses of all 21 strains, we identified genes of lignocellulolytic enzymes with the potential to degrade cellulose (GH5, GH30, GH4, GH1, GH16 and GH32) and hemicellulose (GH43, GH30, GH51, GH26 and GH53). Further identification of the secretome of B. velezensis LC1 by liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmed that a considerable number of proteins in the culture medium are involved in lignocellulose degradation, including endoglucanase, hemicellulases, and other related proteins. Moreover, assays of the activities of several lignocellulolytic enzymes including endoglucanase, exoglucanase, β-glucosidase, xylanase, lignin peroxidase, laccase, and manganese peroxidase show that these enzymes are more active in bamboo powder compared to glucose substrates. After a 6-day treatment, the degradation efficiencies of cellulose, hemicellulose and lignin from bamboo powder were 59.90%, 75.44% and 23.41%, respectively. The hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 10.44 g/L ethanol after 96 h.Conclusions: These findings indicate that Bacillus velezensis strains have the potential to degrade a range of polysaccharides in lignocellulosic biomass. One strain, B. velezensis LC1, efficiently degrades bamboo lignocellulose components, allowing for subsequent ethanol production.

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“…Strain 9912D, with the greatest spore yield and most antagonistic activity against phytopathogenic fungi, had been derived from wild-type 9912 by chemical and physical mutagenesis, which may have resulted in loss of some genes (Pan et al 2017). B. velezensis 157 revealed a significantly higher number of six GH13 (one α-amylase, three α-glucosidase, α-glycosidase and α-α-phosphotrehalase) and CBM34 than that found in genomes of related B. velezensis, which are responsible for hydrolyzing starch (Graebin et al 2016). Meanwhile, B. velezensis 157 with one GH65 (maltose phosphorylase), was expected to be able to degrade trehalose (Inoue et al 2002).…”

Section: Resultsmentioning

confidence: 99%

Comparative genome analysis of Bacillus velezensis reveals a potential for degrading lignocellulosic biomass

Chen

et al. 2018

3 Biotech

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Genomes of 24 sequenced strains were characterized to identity shared and unique genes of lignocellulolytic enzymes and predict potential to degrade lignocellulose. All 24 strains had genes that encoded lignocellulolytic enzymes, with potential to degrade cellulose and hemicelluloses. Several lignocellulosic genes related to cellulose degradation were universally present, including one GH5 (endo-1,4-β-glucanase), one GH30 (glucan endo-1,6-β-glucosidase), two GH4 (6-phospho-β-glucosidase, 6-phospho-α-glucosidase), one GH1 (6-phospho-β-galactosidase), one GH16 (β-glucanase) and three GH32 (two sucrose-6-phosphate hydrolase and levanase). However, in the absence of gene(s) for cellobiohydrolase, it was predicted that none of the 24 strains would be able to directly hydrolyse cellulose. Regarding genes for hemicellulose degradation, four GH43 (1,4-β-xylosidase; except strain 9912D), one GH11 (endo-1,4-β-xylanase), three GH43 (two arabinan endo-1,5-α-L-arabinosidase and one arabinoxylan arabinofuranohydrolase), two GH51 (α-N-arabinofuranosidase), one GH30 (glucuronoxylanase), one GH26 (β-mannosidase) and one GH53 (arabinogalactan endo-1,4-β-galactosidase) were present. In addition, two PL1 (pectate lyase) and one PL9 (pectate lyase) with potential for pectin degradation were conserved among all 24 strains. In addition, all 24 had limited representation of the auxiliary activities super-family, consistent with a limited ability to degrade lignin. Therefore, it was predicted that for these bacteria to degrade lignin, pretreatment of lignocellulosic substrates may be required. Finally, based on in silico studies, we inferred that strains may degrade a range of polysaccharides in lignocellulosic biomasses.

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Immobilization of Glycoside Hydrolase Families GH1, GH13, and GH70: State of the Art and Perspectives

Cited by 49 publications

References 300 publications

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Comparative genomic and secretomic characterisation of endophytic Bacillus velezensis LC1 producing bioethanol from bamboo lignocellulose

Comparative genome analysis of Bacillus velezensis reveals a potential for degrading lignocellulosic biomass

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