Immobilization of extracellular matrix on polymeric materials by carbon-negative-ion implantation

Tsuji, Hiroshi; Sommani, Piyanuch; Muto, Takashi; Utagawa, Yoshiyuki; Sakai, Shun; Sato, Hiroko; Gotoh, Yasuhito; Ishikawa, Junzo

doi:10.1016/j.nimb.2005.05.020

Cited by 17 publications

(27 citation statements)

References 14 publications

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“…The adhesion of cells on the narrower linewidth than cell size was considered to be due to two eŠects. One is the improved adsorption of ECM on the implanted regions and other is the small amount of ECM adsorbed on the interface area between the implanted and unimplanted regions 3,4) . The elongated adhesion along the implanted region and a little spread adhesion around the boundary regions resulted in the observed cell adhesion on the narrower line-width than the cell size.…”

Section: Resultsmentioning

confidence: 99%

“…Improvement of cell adhesions on polymeric materials have been purposed by using ion implantation with both ion-types of positive and negative [1][2][3][4][5] . Negative-ion implantation techniques have been used to modify polymer surface for improvement of cell-a‹nity because of its advantage of``charge-up free'' [6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

“…For cell culture, carbon element is selected because of its harmless to cell and matching to polymer-lattice. As previous work, we modiˆed the hydrophobic polymer of silicone rubber (SR) by using carbon negative-ion implantation [3][4][5] . XPS analysis showed the formations of oxygen functional groups such as carbonyl and hydroxyl on the ion-induced defects after the implantation 4) .…”

Section: Introductionmentioning

confidence: 99%

“…Such formed groups having negative-polar characteristic refer to the hydrophilic properties, leading to the lowering of water contact angle of the implanted SR from about 100°to 86°3 ,4) . These eŠects promoted the adsorption of extracellular matrix (ECM), necessary adhesive proteins for cell adhesion, on the implanted area 3,4) . By using micro-pattern mask with slit-aperture width of 50 mm, we could pattern the adhesions of nerve cells and mesenchymal stem cells (MSC) on the implanted regions 3,5) .…”

Section: Introductionmentioning

confidence: 99%

“…These eŠects promoted the adsorption of extracellular matrix (ECM), necessary adhesive proteins for cell adhesion, on the implanted area 3,4) . By using micro-pattern mask with slit-aperture width of 50 mm, we could pattern the adhesions of nerve cells and mesenchymal stem cells (MSC) on the implanted regions 3,5) . Moreover, we had investigated the trial diŠerentiation of MSC into neuron with retaining adhesion pattern 5) .…”

Section: Introductionmentioning

confidence: 99%

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Fine Adhesion Patterning and Aligned Nuclei Orientation of Mesenchymal Stem Cell on Narrow Line-Width of Silicone Rubber Implanted by Carbon Negative Ions

et al. 2010

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Fine cell-adhesion patterning and nuclei orientation control on silicone rubber were investigated by limiting line-width of carbon negative-ion implantation. The ions were implanted at 3×10 15 ions/cm 2 and 10 keV through masks of ridge-pattern and rectangle-pattern with various slit apertures of 0-40 mm in width. After 2 days culture, nuclei of rat mesenchymal stem cells were stained with ‰uorescent dye to evaluate the nuclei positions at each tip ridge-region and the relative angles between the major axis of ellipse-shape nuclei and the implanted line-region. Results showed the individual nucleus arrangement along the narrow line-widths of 3-12 mm and the gathered one on the wider line-width. Number of nuclei orientation in the angle range of 0°-10°increased to 79z on the line-width of 10 mm. As a result, theˆne cell-adhesion patterning and nuclei orientation could be achieved by decreasing the implanted line-width to 10 mm.

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Section: Resultsmentioning

confidence: 99%