Fine Adhesion Patterning and Aligned Nuclei Orientation of Mesenchymal Stem Cell on Narrow Line-Width of Silicone Rubber Implanted by Carbon Negative Ions

Abstract

Fine cell-adhesion patterning and nuclei orientation control on silicone rubber were investigated by limiting line-width of carbon negative-ion implantation. The ions were implanted at 3×10 15 ions/cm 2 and 10 keV through masks of ridge-pattern and rectangle-pattern with various slit apertures of 0-40 mm in width. After 2 days culture, nuclei of rat mesenchymal stem cells were stained with ‰uorescent dye to evaluate the nuclei positions at each tip ridge-region and the relative angles between the major axis of ellipse-shape nuclei and the implanted line-region. Results showed the individual nucleus arrangement along the narrow line-widths of 3-12 mm and the gathered one on the wider line-width. Number of nuclei orientation in the angle range of 0°-10°increased to 79z on the line-width of 10 mm. As a result, theˆne cell-adhesion patterning and nuclei orientation could be achieved by decreasing the implanted line-width to 10 mm.