Abstract:Immobilization of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from spinach leaves is described. This enzyme enables the fixation of carbon dioxide on a five-carbon sugar D-ribulose-1,5-bisphosphate (RuBP). Two different immobilization methods were employed: dicyclohexylcarbodiimide coupling on nylon membrane matrix and dimethylpimelimidate immobilization on protein A agarose. The reusability of immobilized enzymes, coupling efficiency, and temperature-activity relationship of soluble and immobi… Show more
“…A bioprocess enabling fixation of CO 2 in bioreactors employing recombinant Rubisco has been invented [17,18]. In this approach, modular devices are used, where in one module the CO 2 is fixed on RuBP ( d ‐ribulose 1,5‐bisphosphate) using Rubisco [18,19] and in the other module a cohort of enzymes regenerates RuBP from 3‐phospho‐glycerate. The bioprocess for fixation of Rubisco employs a highly enriched and relatively purified stream of CO 2 obtained after treatment of the emission stream.…”
The enzyme carbonic anhydrase (isoform II) from bovine and human erythrocytes was immobilized using different covalent coupling methods on inert matrices. Immobilized carbonic anhydrase may enable concentration of CO2 for Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)-catalysed fixation in bioreactors. In the present study the activity of carbonic anhydrase with respect to hydration of CO2 using soluble and immobilized enzymes was determined. The stability of the immobilization matrix, the properties of the immobilized enzymes subjected to a variation in operation variables and the activity profile with respect to storage are reported. Immobilization imparted greater thermal and storage stability and enhanced reusability.
“…A bioprocess enabling fixation of CO 2 in bioreactors employing recombinant Rubisco has been invented [17,18]. In this approach, modular devices are used, where in one module the CO 2 is fixed on RuBP ( d ‐ribulose 1,5‐bisphosphate) using Rubisco [18,19] and in the other module a cohort of enzymes regenerates RuBP from 3‐phospho‐glycerate. The bioprocess for fixation of Rubisco employs a highly enriched and relatively purified stream of CO 2 obtained after treatment of the emission stream.…”
The enzyme carbonic anhydrase (isoform II) from bovine and human erythrocytes was immobilized using different covalent coupling methods on inert matrices. Immobilized carbonic anhydrase may enable concentration of CO2 for Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)-catalysed fixation in bioreactors. In the present study the activity of carbonic anhydrase with respect to hydration of CO2 using soluble and immobilized enzymes was determined. The stability of the immobilization matrix, the properties of the immobilized enzymes subjected to a variation in operation variables and the activity profile with respect to storage are reported. Immobilization imparted greater thermal and storage stability and enhanced reusability.
“…The process of carbon dioxide fixation can be carried out successfully with a pure stream of carbon dioxide in bioreactor [105]. In order to make the bioprocess feasible the enzyme d-Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was recently immobilized [106]. Also, the novel trickling spray reactor employing immobilized carbonic anhydrase, which enables concentration of CO 2 from the emission stream was developed [107] Carbonic anhydrase is one of the fastest enzymes that make fast mass transfer from gas phase to aqueous phase.…”
Section: Advantages Of Biotransformations Over Classical Chemistry Enmentioning
Throughout the history of mankind, microorganisms have been of enormous social and economic importance. Without even being aware of their existence, very early on in history man was using them in the production of food and beverages. The Sumerians and Babylonians were practising the brewing of beer before 6000 BC, references to wine making can be found in the Book of Genesis and the Egyptians used yeast for baking bread. However, knowledge of the production of chemicals such as alcohols and organic acids through fermentation is relatively recent and the first reports in the literature only appeared in the second half of the 19th century. Lactic acid was probably the first optically active compound to be produced industrially by fermentation. This was accomplished in the USA in 1880 [1]. In 1921, Chapman reviewed a number of early industrial fermentation processes for organic chemicals [2].In the course of time, it was discovered that microorganisms could modify certain compounds by simple, chemically well defined reactions, which were further catalyzed by enzymes. Nowadays, these processes are called "biotransformations". The essential difference between fermentation and biotransformation is that there are several catalytic steps between the substrate and the product in a fermentation while there are only one or two in a biotransformation. The distinction is also in the fact that the chemical structures of the substrate and the product resemble one another in a biotransformation, but not necessarily in a fermentation.
“…Subsequent to capture the carbon dioxide becomes solublized (as carbonic acid or bicarbonate). After adjustment of pH using controllers and pH-stat the solution is fed to immobilized Rubisco reactors [18] where acceptor D-Ribulose-1,5-bisphosphate (RuBP) after CO 2 fixation is converted into 3-phosphoglycerate [16,17]. However, inasmuch as the recycling of acceptor RuBP is central to continuous CO 2 fixation, we have invented a novel scheme (Figure 1), which proceeds with no loss of CO 2 (unlike cellular biochemical systems) in 11 steps in a series of bioreactors [20].…”
Sugar binding proteins and binders of intermediate sugar metabolites derived from microbes are increasingly being used as reagents in new and expanding areas of biotechnology.
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