Immobilization of cellulase on thermo-sensitive magnetic microspheres: improved stability and reproducibility

Abstract

Magnetic double-shell hybrid microspheres (FeO@SiO@p(NIPAM-co-GMA)) have been developed as a promising supported substrate for the immobilization of cellulase. Since the surface of the magnetic microspheres not only contains an epoxy group from GMA (glycidyl methacrylate) that can covalently bind to the enzyme, but also has an intelligent temperature response property from NIPAM (N-isopropylacrylamide), the cellulase can be covalently bonded to the magnetic microspheres and have a temperature-sensitive capability. The immobilized cellulase has the recovery ability of cellulase activity after a high-temperature inactivation. The average amount and activity of immobilized enzymes, respectively, was 233 mg g, 57.4 U mg under the optimized conditions. The experimental results show that the immobilized cellulase has a wider catalytic temperature range, better temperature and storage stability. The residual activity still remained about 65.6% of the initial activity after the sixth catalysis run, which indicated that the immobilized enzyme had high reproducibility.

A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization

“…These evidences proved the high activity of BcHep-I could attribute to its unique molecular structure. Taking all the results into account, the stability of BcHep-I could be further promoted which may be achieved through protein engineering or immobilization [38][39][40] in the future. Therefore, the application prospect of heparinase I is broad and promising.…”

Preliminary Study of Reducing Sugar Production from Coconut Husk by Enzymatic Hydrolysis Using Chitosan Immobilized Crude and Commercial Cellulase

“…The competitive result was shown although the micro-sized and the nano-sized had less coupled. It was due to smaller size particle provide larger surface area and numerous active sites available for the cellulose molecules to be coupled [10,14] .…”

Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review

“…Due to the unique molecular structure and functional group characteristics of LCST polymer, researchers can effectively use the change of ambient temperature to control the phase behavior of materials and then realize the intelligentization of materials. Now, LCST polymer has been applied to biomedical, catalytic, sensor, immobilized enzymes, and other fields. In the field of immobilized enzymes, Ke Li et al synthesized the tri‐block temperature‐sensitive polymer PDEA‐b‐PHEMA‐b‐PGMA and applied it to the PGA immobilization carrier for the first time, successfully using the polymer sol state realize the immobilization of the PGA and the catalytic conversion of the immobilized PGA, which effectively increased the mass transfer rate, and the recovery of the immobilized PGA in the gel state (Figure ).…”