Immobilization of Bacterial Cells in Hydrogels Prepared by Gamma Irradiation for Bioremoval of Strontium Ions

Singh, Rita; Shitiz, Kirti; Singh, Arvind Kumar

doi:10.1007/s11270-019-4374-8

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…We propose and demonstrate a protocol for 3D QPI of bacteria that does not suffer from motion-induced artifacts or background noise. Our protocol utilizes hydrogel as part of the loading medium and benefits from the formation of optically clear extracellular matrices 34 , 35 . The hydrogel facilitates high-quality 3D QPI measurements of bacteria by immobilizing bacteria without generating noise.…”

Section: Discussion and Summarymentioning

confidence: 99%

“…To achieve 3D QPI measurements of bacteria with neither motion-induced problems nor background noise, we propose an experimental method that utilizes a hydrogel to improve the imaging quality. Hydrogels are cross-linked hydrophilic polymers that form optically clear extracellular matrices and are suitable materials for addressing the aforementioned issues 34 , 35 . Here, we experimentally demonstrate a method for imaging bacteria using hydrogel substrates and optical diffraction tomography (ODT), a 3D QPI technique 36 .…”

Section: Introductionmentioning

confidence: 99%

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Long-term label-free assessments of individual bacteria using three-dimensional quantitative phase imaging and hydrogel-based immobilization

Shin

Kim

Park

et al. 2023

Sci Rep

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Three-dimensional (3D) quantitative phase imaging (QPI) enables long-term label-free tomographic imaging and quantitative analysis of live individual bacteria. However, the Brownian motion or motility of bacteria in a liquid medium produces motion artifacts during 3D measurements and hinders precise cell imaging and analysis. Meanwhile, existing cell immobilization methods produce noisy backgrounds and even alter cellular physiology. Here, we introduce a protocol that utilizes hydrogels for high-quality 3D QPI of live bacteria maintaining bacterial physiology. We demonstrate long-term high-resolution quantitative imaging and analysis of individual bacteria, including measuring the biophysical parameters of bacteria and responses to antibiotic treatments.

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Section: Discussion and Summarymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long-term label-free assessments of individual bacteria using three-dimensional quantitative phase imaging and hydrogel-based immobilization

Shin

Kim

Park

et al. 2023

Sci Rep

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“…Some other radionuclides, such as Eu 3+ , Th 4+ , TcO 4 − , ReO 4 − , 129 I − , Co 2+ , Cs + , and Sr 2+ , are also highly hazardous to human health. 83 , 84 , 85 , 86 In recent years, COF-based materials have shown a great potential in the preconcentration of radionuclides from radioactive wastewater.…”

Section: Removal Of Radionuclides By Cof-based Materialsmentioning

confidence: 99%

“…These advantages, together with the characteristics of predesigned structure, ordered porous structure, and adjustable physicochemical properties, make COFs promising next-generation materials with remarkable high sorption capacities for radioactive/toxic metal ions and organic contaminants. 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 …”

Section: Introductionmentioning

confidence: 99%

Orderly Porous Covalent Organic Frameworks-based Materials: Superior Adsorbents for Pollutants Removal from Aqueous Solutions

et al. 2021

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Summary Covalent organic frameworks (COFs) are a new type of crystalline porous polymers known for chemical stability, excellent structural regularity, robust framework, and inherent porosity, making them promising materials for capturing various types of pollutants from aqueous solutions. This review thoroughly presents the recent progress and advances of COFs and COF-based materials as superior adsorbents for the efficient removal of toxic heavy metal ions, radionuclides, and organic pollutants. Information about the interaction mechanisms between various pollutants and COF-based materials are summarized from the macroscopic and microscopic standpoints, including batch experiments, theoretical calculations, and advanced spectroscopy analysis. The adsorption properties of various COF-based materials are assessed and compared with other widely used adsorbents. Several commonly used strategies to enhance COF-based materials’ adsorption performance and the relationship between structural property and sorption ability are also discussed. Finally, a summary and perspective on the opportunities and challenges of COFs and COF-based materials are proposed to provide some inspiring information on designing and fabricating COFs and COF-based materials for environmental pollution management.

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“…This benign environment can suppress outside noise, thereby improving sensing signals [ 31 ]. Singh et al immobilised five strains of strontium-resistant bacteria into an acrylamide hydrogel polymer and found that the immobilised microbes had a higher rate of strontium removal efficiency [ 32 ].…”

Section: Hydrogels Used In Metsmentioning

confidence: 99%

Recent Implementations of Hydrogel-Based Microbial Electrochemical Technologies (METs) in Sensing Applications

Wang

Shi

et al. 2023

Sensors

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Hydrogel materials have been used extensively in microbial electrochemical technology (MET) and sensor development due to their high biocompatibility and low toxicity. With an increasing demand for sensors across different sectors, it is crucial to understand the current state within the sectors of hydrogel METs and sensors. Surprisingly, a systematic review examining the application of hydrogel-based METs to sensor technologies has not yet been conducted. This review aimed to identify the current research progress surrounding the incorporation of hydrogels within METs and sensors development, with a specific focus on microbial fuel cells (MFCs) and microbial electrolysis cells (MECs). The manufacturing process/cost, operational performance, analysis accuracy and stability of typical hydrogel materials in METs and sensors were summarised and analysed. The current challenges facing the technology as well as potential direction for future research were also discussed. This review will substantially promote the understanding of hydrogel materials used in METs and benefit the development of electrochemical biosensors using hydrogel-based METs.

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Immobilization of Bacterial Cells in Hydrogels Prepared by Gamma Irradiation for Bioremoval of Strontium Ions

Cited by 6 publications

References 38 publications

Long-term label-free assessments of individual bacteria using three-dimensional quantitative phase imaging and hydrogel-based immobilization

Long-term label-free assessments of individual bacteria using three-dimensional quantitative phase imaging and hydrogel-based immobilization

Orderly Porous Covalent Organic Frameworks-based Materials: Superior Adsorbents for Pollutants Removal from Aqueous Solutions

Recent Implementations of Hydrogel-Based Microbial Electrochemical Technologies (METs) in Sensing Applications

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