Immobilization-free DNA-based homogeneous electrochemical biosensors

Zhang, Fangting; Cai, Liangyuan; Zhou, Ying‐Lin; Zhang, Xin‐Xiang

doi:10.1016/j.trac.2016.08.012

Cited by 54 publications

(22 citation statements)

References 151 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zwitterionic amphoteric surfactants produce a positive or negative charge depending on the pH value of the solution (Rekha et al, 2009). Surfactants as organized assemblies are significantly applied in ordinary life, chemical industry, medical science, material processing, and the other significant scope such as nano reactors, drug carriers, and fabrication of sensors (Eivazzadeh‐Keihan et al, 2018; Hayes & Smith, 2019; Jain & Rather, 2011b; Le Guenic et al, 2019; Li, Luo, et al, 2015; Zahid et al, 2017; Zhang, Cai, et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Today, electrochemical sensors have become an essential category of chemical sensors, which have many advantages, including suitable field‐based size, absolute discrimination ability, and fast response (Afzali et al, 2016; Hatami et al, 2019; Housaindokht et al, 2018; Nasiri et al, 2021; Zahid et al, 2017). They have received much consideration, both in fundamental research and practical applications, because of their several benefits such as easy construction, reasonable cost, outstanding detection limits with sufficiency to the complicated biofluids (Eivazzadeh‐Keihan et al, 2018; Zhang, Cai, et al, 2016). Voltammetry is an important electrochemical technique on which many of the electrochemical sensors relies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in applications of surfactant‐based voltammetric sensors

Housaindokht

Janati-Fard

Ashraf

2021

J Surfact & Detergents

View full text Add to dashboard Cite

Voltammetric sensors are simple, powerful, and cheap equipment with easy principles, which have been widely used in electrochemical studies to investigate the properties of the electroactive species. Surfactants are frequently used in the construction of voltammetric sensors to enhance the sensitivity and selectivity of the sensor, which significantly impacted the electrode function in terms of dissolving organic compounds, creating a specific orientation of molecules on the surface of the electrodes, and enhancing electron transfer reactions. This review focuses on the recent contributions of surfactants in the development of efficient voltammetric sensors. In addition, other ingredients commonly employed alongside surfactants, such as nanomaterials, conducting polymers, and composite materials, have been discussed. The comprehension of these aspects can guide the expansion and the use of surfactants in the electrochemical sensing scope.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recent advances in applications of surfactant‐based voltammetric sensors

Housaindokht

Janati-Fard

Ashraf

2021

J Surfact & Detergents

View full text Add to dashboard Cite

show abstract

“…Therefore, extensive efforts have been devoted to developing techniques for detecting highly sensitive sequence-specific DNA sensors based on optical [6,7], electrical [8,9] and electrochemical [1,3,10,11] methods. Among these techniques, electrochemical methods have received considerable attention because they can be used to fabricate fast, simple, highly sensitive, and miniature DNA sensors [12,13]. Electrochemical impedance spectroscopy (EIS) is a very accurate electrochemical method, which is sensitive to changes on the electrode surface and is suitable for label-free DNA detection [14,15].…”

Section: Introductionmentioning

confidence: 99%

Physical Surface Modification of Carbon-Nanotube/Polydimethylsiloxane Composite Electrodes for High-Sensitivity DNA Detection

2021

View full text Add to dashboard Cite

The chemical modification of electrode surfaces has attracted significant attention for lowering the limit of detection or for improving the recognition of biomolecules; however, the chemical processes are complex, dangerous, and difficult to control. Therefore, instead of the chemical process, we physically modified the surface of carbon-nanotube/polydimethylsiloxane composite electrodes by dip coating them with functionalized multi-walled carbon nanotubes (F-MWCNTs). These electrodes are used as working electrodes in electrochemistry, where they act as a recognition layer for sequence-specific DNA sensing through π–π interactions. The F-MWCNT-modified electrodes showed a limit of detection of 19.9 fM, which was 1250 times lower than that of pristine carbon/polydimethylsiloxane electrodes in a previous study, with a broad linear range of 1–1000 pM. The physically modified electrode was very stable during the electrode regeneration process after DNA detection. Our method paves the way for utilizing physical modification to significantly lower the limit of detection of a biosensor system as an alternative to chemical processes.

show abstract

“…Cucurbit[8]uril (CB[8]) is an important host macromolecule that has been used for protein and cell immobilization. [ 16–20 ] The carbonyl‐fringed portals and the hydrophobic cavity allows CB[n]s to form stable host–guest complexes with various molecules. Due to its large dimensions, CB[8] is a suitable host for a wide range of organic molecules, and is considered as an excellent candidate as an electrode modifier.…”

Section: Introductionmentioning

confidence: 99%

An electrogenerated chemiluminescence aptasensor for lysozyme based on the interaction between Ru(bpy)₃²⁺ and cucurbit[8]uril

Yin

Dong

et al. 2020

Luminescence

View full text Add to dashboard Cite

Strong anodic Ru(bpy)32+ electrogenerated chemiluminescence (ECL) was obtained at a cucurbil[8]uril (CB[8]) modified electrode in neutral conditions without the need of an additional coreactant. An ECL aptasensor was fabricated based on the strong ECL emission as well as the host–guest interaction between DNA and CB[8]. Firstly, amino group‐terminated complementary DNA (DNA‐NH2) was firmly immobilized on CB[8]/glass carbon electrode, which could further increase ECL intensity. Then, a ferrocene group‐terminated lysozyme aptamer (Fc‐DNA) was hybridized with complementary DNA. The inhibiting effect of ferrocene on Ru(bpy)32+ ECL resulted in the apparent decrease in ECL signal. When the modified electrode was incubated in lysozyme, specific binding between lysozyme and its aptamer could release the ferrocene group from the electrode surface, and the ECL emission was recovered. As a result, an ‘on–off–on’ mode ECL aptasensor for lysozyme was fabricated. In the range 0.14–140 pg ml−1, the increased ECL intensities exhibited excellent linearity with the logarithm of lysozyme concentrations, and the detection limit was calculated as 0.093 pg ml−1 (3σ). The proposed ECL aptasensor exhibited satisfactory analytical performance, revealing the potential application of CB[n]s in an ECL sensing field.

show abstract

Immobilization-free DNA-based homogeneous electrochemical biosensors

Cited by 54 publications

References 151 publications

Recent advances in applications of surfactant‐based voltammetric sensors

Recent advances in applications of surfactant‐based voltammetric sensors

Physical Surface Modification of Carbon-Nanotube/Polydimethylsiloxane Composite Electrodes for High-Sensitivity DNA Detection

An electrogenerated chemiluminescence aptasensor for lysozyme based on the interaction between Ru(bpy)₃²⁺ and cucurbit[8]uril

Contact Info

Product

Resources

About

Immobilization-free DNA-based homogeneous electrochemical biosensors

Cited by 54 publications

References 151 publications

Recent advances in applications of surfactant‐based voltammetric sensors

Recent advances in applications of surfactant‐based voltammetric sensors

Physical Surface Modification of Carbon-Nanotube/Polydimethylsiloxane Composite Electrodes for High-Sensitivity DNA Detection

An electrogenerated chemiluminescence aptasensor for lysozyme based on the interaction between Ru(bpy)32+ and cucurbit[8]uril

Contact Info

Product

Resources

About

An electrogenerated chemiluminescence aptasensor for lysozyme based on the interaction between Ru(bpy)₃²⁺ and cucurbit[8]uril