An electrogenerated chemiluminescence aptasensor for lysozyme based on the interaction between Ru(bpy)₃²⁺ and cucurbit[8]uril

Abstract

Strong anodic Ru(bpy)32+ electrogenerated chemiluminescence (ECL) was obtained at a cucurbil[8]uril (CB[8]) modified electrode in neutral conditions without the need of an additional coreactant. An ECL aptasensor was fabricated based on the strong ECL emission as well as the host–guest interaction between DNA and CB[8]. Firstly, amino group‐terminated complementary DNA (DNA‐NH2) was firmly immobilized on CB[8]/glass carbon electrode, which could further increase ECL intensity. Then, a ferrocene group‐terminated lysozyme aptamer (Fc‐DNA) was hybridized with complementary DNA. The inhibiting effect of ferrocene on Ru(bpy)32+ ECL resulted in the apparent decrease in ECL signal. When the modified electrode was incubated in lysozyme, specific binding between lysozyme and its aptamer could release the ferrocene group from the electrode surface, and the ECL emission was recovered. As a result, an ‘on–off–on’ mode ECL aptasensor for lysozyme was fabricated. In the range 0.14–140 pg ml−1, the increased ECL intensities exhibited excellent linearity with the logarithm of lysozyme concentrations, and the detection limit was calculated as 0.093 pg ml−1 (3σ). The proposed ECL aptasensor exhibited satisfactory analytical performance, revealing the potential application of CB[n]s in an ECL sensing field.

Chemiluminescence assay for kanamycin based on target recycling strategy

“…Aptamers are single-stranded DNA or RNA oligonucleotides with high specificity and affinity for target substances screened from random oligonucleotide libraries based on Systematic Evolution of Ligands by EXponential enrichment (SELEX). [9][10][11] As an excellent tool of biological science, the aptamer has been widely used in the detection of cells, viruses, proteins, toxins, allergens, and other biological components because of its high affinity, strong specificity, large surface area, a large number of receptor binding sites, and a wide range of targets. [12][13][14] Catalytic hairpin assemblies (CHA), as a mature enzyme-free DNA amplification technique with high efficiency and target cycle, have been applied in the field of signal transduction and amplification related to nucleic acid analysis and biosensor.…”

Electrogenerated chemiluminescence sensor for silver ions based on their coordination interaction with cucurbit[6]uril

“…Thus, some preliminary investigations have been carried out in our laboratory, and it was found that Q[ n ]s could enhance the Ru(bpy) 3 2+ ECL intensity, which was used to detect lysozyme. 26 However, there are few reports about the application of Q[ n ]s in fabricating ECL sensors, and further applications of Q[ n ]s in ECL still need to be explored.…”