Immobilised cells of Pachysolen tannophilus yeast for ethanol production from crude glycerol

Stepanov, Nikolay; Ефременко, Елена

doi:10.1016/j.nbt.2016.05.002

Cited by 42 publications

(16 citation statements)

References 18 publications

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“…This was confirmed by the fact that, in the third cycle of cell usage, the same characteristics of methanogenesis were observed as in the second cycle. The observed effects were associated with the improvement of the adaptation of bacterial immobilized biocatalysts to the culture conditions, as observed in other studies [13,15,16,17].…”

Section: Resultssupporting

confidence: 77%

“…One of the advantages of using cells immobilized in a PVA cryogel demonstrated in this process is the possibility of the repeated use of these cells without losing efficacy, which was previously demonstrated only for other cells [13,15,16,17]. The results obtained open up prospects for a more in-depth study of the proposed approaches and may be useful to researchers engaged in the study of issues of environmentally safe and cost-effective waste treatment with the production of commercially significant products.…”

Section: Discussionmentioning

confidence: 99%

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Prospective Approach to the Anaerobic Bioconversion of Benzo- and Dibenzothiophene Sulfones to Sulfide

et al. 2019

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Sulfur recovery from organic molecules such as toxic sulfones is an actual problem, and its solution through the use of environmentally friendly and nature-like processes looks attractive for research and application. For the first time, the possible bioconversion of organic sulfones (benzo-and dibenzothiophene sulfones) to inorganic sulfide under anaerobic conditions with simultaneous biogas production from glucose within a methanogenesis process is demonstrated. Biogas with a methane content of 50.7%–82.1% was obtained without H2S impurities. Methanogenesis with 99.7%–100% efficiency and 97.8%–100% conversion of benzo- and dibenzothiophene sulfones (up to 0.45 mM) to inorganic sulfide were obtained in eight days by using a combination of various anaerobic biocatalysts immobilized in a poly(vinyl alcohol) cryogel. Pure cell cultures of sulfate-reducing bacteria and/or H2-producing bacteria were tested as additives to the methanogenic activated sludge. The immobilized activated sludge “enhanced” by bacterial additives appeared to retain its properties and be usable multiple times for the conversion of sulfones under batch conditions.

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Section: Resultssupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Prospective Approach to the Anaerobic Bioconversion of Benzo- and Dibenzothiophene Sulfones to Sulfide

et al. 2019

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“…The cell metabolic activity was determined by measuring the intracellular ATP concentration in samples of anaerobic consortia using the bioluminescent luciferin-luciferase method and the Microluminometer 3560 (New Horizons Diagnostics Co, Baltimore, MD, USA) [17,21,26]. For this purpose, samples of fermentation medium after vigorous mixing were collected at the beginning and end of the process (0.1 mL), transferred to dimethyl sulfoxide (0.9 mL), and allowed to stand at 25 • C for 2 h to extract intracellular ATP.…”

Section: Methodsmentioning

confidence: 99%

A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis

et al. 2019

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The possible use of the concentration of intracellular adenosine triphosphate (ATP) as a parameter enabling quick and adequate evaluation of the metabolic activity of methanogenic cells was demonstrated in the work. This approach was used to analyze the effect of introducing potassium humate and fulvic acids (1-10 g/L) into media with four different methanogenic consortia producing biogas. The ATP concentration was analyzed by the bioluminescent luciferin-luciferase method at the beginning and end of the process. During the entire process, the biogas composition, biogas efficiency, and the kinetics of methanogenesis in the presence of humic compounds were determined. The increase in the concentration of potassium humate led to a decrease in the overall energy status of the cells and reduced methanogenesis efficiency. However, fulvic acids introduced into the media stimulated methanogenesis in half of the tested consortia, which was accompanied by an increase in ATP concentration in cell samples. So, a positive correlation between the metabolic activity of cells in biogas formation and the concentration of ATP was observed. ATP concentration control appears to be an attractive tool for finding compounds that suppress methanogenesis in landfills.

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“…Glycerol is a useful raw material for the production of value added chemicals, in particular through bioconversion by microbial fermentation. A number of such promising applications include conversion to 1,3-propanediol, 9,10,[14][15][16] succinate, 17 ethanol, 18,19 butanol 20 and hydrogen. 21 The product of interest in this study is 1,3-propanediol, as we have shown that 1,3-PD can be converted into a range of value-added chemicals in downstream chemocatalytic hydrogen borrowing reactions.…”

Section: Introductionmentioning

confidence: 99%

Utilizing Crude Waste Glycerol in the Biorefinery: Glycerol Gels for in Situ Substrate Delivery to Whole Cell Biocatalysts

Bothwell

Krasňan

Lorenzini

et al. 2019

ACS Sustainable Chem. Eng.

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Immobilised cells of Pachysolen tannophilus yeast for ethanol production from crude glycerol

Cited by 42 publications

References 18 publications

Prospective Approach to the Anaerobic Bioconversion of Benzo- and Dibenzothiophene Sulfones to Sulfide

Prospective Approach to the Anaerobic Bioconversion of Benzo- and Dibenzothiophene Sulfones to Sulfide

A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis

Utilizing Crude Waste Glycerol in the Biorefinery: Glycerol Gels for in Situ Substrate Delivery to Whole Cell Biocatalysts

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