A New Approach to Assess the Effect of Various Humic Compounds on the Metabolic Activity of Cells Participating in Methanogenesis

Abstract

The possible use of the concentration of intracellular adenosine triphosphate (ATP) as a parameter enabling quick and adequate evaluation of the metabolic activity of methanogenic cells was demonstrated in the work. This approach was used to analyze the effect of introducing potassium humate and fulvic acids (1-10 g/L) into media with four different methanogenic consortia producing biogas. The ATP concentration was analyzed by the bioluminescent luciferin-luciferase method at the beginning and end of the process. During the entire process, the biogas composition, biogas efficiency, and the kinetics of methanogenesis in the presence of humic compounds were determined. The increase in the concentration of potassium humate led to a decrease in the overall energy status of the cells and reduced methanogenesis efficiency. However, fulvic acids introduced into the media stimulated methanogenesis in half of the tested consortia, which was accompanied by an increase in ATP concentration in cell samples. So, a positive correlation between the metabolic activity of cells in biogas formation and the concentration of ATP was observed. ATP concentration control appears to be an attractive tool for finding compounds that suppress methanogenesis in landfills.

Metal Nanoparticles for Improving Bactericide Functionality of Usual Fibers

“…Benzethonium and benzalkonium chlorides were used as reference compounds in MBC detection. The concentration of intracellular ATP was determined by the known luciferin-luciferase method [12] with a Microluminometr 3560 (New horizons diagnostics, Arbutus, MD, USA) using a standard ATP reagent based on recombinant firefly luciferase (Lumtek LLC, Moscow, Russia). The data were linearized in semi-logarithm coordinates [11].…”

“…To solve the issue, specific dyes [9] or even a panel of direct detection bioassays [10] could be adapted. Alternatively, the concentration of intracellular adenosine triphosphate (ATP) [11,12] could be measured to produce highly reproducible and relevant results with nanomaterials [13].…”

Influence of humic compounds on bioluminescence of immobilized photobacteria

“…It was shown that in the studied range of concentrations of HCs (0.1-10 g / l) there is a notable direct correlation between the concentration of HCs and the residual luminescence intensity of immobilized P. phosphoreum cells. Moreover, those HCs that had the maximum effect in the case of suppression of the methanogenesis process [5], maximally inhibited the luminescence of photobacteria Thus, it can be argued that the biosensitive element in the form of immobilized P. phosphoreum cells can be used a primary screening tool for assessing the effect of HCs on cells of microorganisms.…”

Regulation of methanogenesis over introducing of humic compounds to biocatalytic systems with complicated content

“…Recently, the inhibition of the methanogenic activity of anaerobic consortia by various humic compounds (HCs) containing various functional (carboxyl, phenolic, hydroxyl, etc.) groups, has been actively studied [2]. At the same time, it should be noted that under real conditions (in landfills), microorganisms involved in the formation of biogas are in a stable state due to both the formation of a stable consortia, high cell density, and immobilization (sorption predominantly) on various materials.…”