Image mapping spectrometry: calibration and characterization

Abstract

Image mapping spectrometry (IMS) is a hyperspectral imaging technique that simultaneously captures spatial and spectral information about an object in real-time. We present a new calibration procedure for the IMS as well as the first detailed evaluation of system performance. We correlate optical components and device calibration to performance metrics such as light throughput, scattered light, distortion, spectral image coregistration, and spatial/spectral resolution. Spectral sensitivity and motion artifacts are also evaluated with a dynamic biological experiment. The presented methodology of evaluation is useful in assessment of a variety of hyperspectral and multi-spectral modalities. Results are important to any potential users/developers of an IMS instrument and to anyone who may wish to compare the IMS to other imaging spectrometers.

Snapshot 3D optical coherence tomography system using image mapping spectrometry

“…This challenge can be overcome by utilizing a tool with a narrower included angle, thus maintaining the uniformity of the facet width. By using a tool with 5 o included angle, the facets width variability was measured to be within 6.7%, in good agreement with previous mappers quality [27].…”

“…Our previously reported IMS systems demonstrated imaging in live biological tissue [27], therefore, we believe there are no system-related limitations for future live biological tissue imaging with IMS-OCT . Currently for our 16 Megapixel camera in this proof-of-concept system, It typically takes 8 s to transfer all acquired data to the computer via a USB port, and 9 s to generate a 3D (x,y,z) volume using Matlab, on an Intel® Core T M 2 Duo chip.…”

“…Since general IMS modalities have been previously reported in literature [24][25][26][27][28], key redesigns to meet OCT imaging' criteria are highlighted.…”

“…These components will be replaced by polarizing beamsplitters and waveplates to circulate light from the source to the detector (via the sample) more efficiently. At the IMS stage, we are designing the next system with a different geometry, aiming to reach light efficiency at the level of 50-60% achieved in previous IMS systems [27].…”

Lenslet array tunable snapshot imaging spectrometer (LATIS) for hyperspectral fluorescence microscopy

“…IFS with mirrors for HFM has been demonstrated with a camera called the Image Mapping Spectrometer (IMS) [35,36]. The IMS has acquired video rate speeds [37] with high light throughput, long pixel dwell time, and low computational costs.…”