Optical hyperspectral imaging in microscopy and spectroscopy - a review of data acquisition

Gao, Liang; Rjh, Smith

doi:10.1002/jbio.201400051

Cited by 161 publications

(103 citation statements)

References 112 publications

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“…The technology is applicable to both laser scanning microscopy and wide-field microscopy [3,4] DOI: 10.1002/opph.201600012…”

Section: Discussionmentioning

confidence: 99%

Innovative Filter Solutions for Hyperspectral Imaging

Pust

2016

Optik & Photonik

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Hyperspectral imaging (HSI) has been used for a couple of decades in applications such as satellite imaging, air reconnaissance and other not overly price sensitive markets. Still, there is no clear definition of hyperspectral imaging. Sometimes techniques that produce 2D images with more than the typical three RGB colors or spectral channels -for example by inclusion of a near-infrared channel -are already called hyperspectral. Mostly though, this is not considered sufficient. Typically, even ten spectral channels are still to be called multi-spectral rather than hyperspectral.In the following, we require that certain criteria are fulfilled for an imaging technique to be hyperspectral: ■ ■ For every pixel in the image, we measure the spectrum of the incident light or radiation.■ ■ The measured spectrum is continuous and not discretized to a limited number of channels.■ ■ The spectrum covers more than one sub-wavelength range, for example UVA, visible and near-infrared or NIR and SWIR. The advent of alternative approaches makes HSI attractive for volume markets or even consumer products, for example cancer detection, precision farming with UAV or directly at the plant, food testing in supermarkets and many more.Alternative approaches comprise sensors that are coated at wafer-level with fixed wavelength bandpass filters, e.g. Pixelteq or imec. Common are also thin film coatings on glass substrates that can be patterned during deposition (in situ), or by using a photolithographic process over the coating to block the addition or subtraction of materials deposited on the substrate surface, e.g. Materion. These micro-patterning techniques allow either filters that have a staircase of different center wavelengths in one direction, suited for the so-called push broom technique, or 2D mosaics, suited for the so-called snapshot technique.However, according to the above criteria, strictly speaking these solutions do not provide hyperspectral capability but are inherently multispectral due to their discrete changes in center wavelength. Typically, between 10 and 100 different wavelengths or channels are offered. A truly hyperspectral sensor offers a continuous change in center wavelength and as such a virtually unlimited number of channels.Delta Optical Thin Film follows a different approach to filters for hyperspectral imaging. Based on our extensive experience with linear variable filters, we develop and manufacture custom linear variable bandpass filters (LVBPF) for mid-size and full-frame CCD/CMOS sensors (e.g. 25 mm × 25 mm or 24 mm × 36 mm). These filters offer very high transmission levels and are fully blocked in the light-sensitive wavelength range of silicon-based detectors (200 nm to 1150 nm or higher). The combination of LVBPFs with silicon detectors allows the design of very compact, robust and affordable HSI detectors that offer sev- Delta Optical Thin Film A/S is the leading supplier of advanced, high performance linear variable filters commonly used in a variety of biomedical imaging applications including...

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“…The technology is applicable to both laser scanning microscopy and wide-field microscopy [3,4] DOI: 10.1002/opph.201600012…”

Section: Discussionmentioning

confidence: 99%

Innovative Filter Solutions for Hyperspectral Imaging

Pust

2016

Optik & Photonik

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“…Hyperspectral imaging (HSI) is also currently emerging as a major tool to afford automated unbiased, non-invasive monitoring of cellular vibration patterning [71,72]. HSI provides measurement of the electromagnetic radiation reflected from an object or scene (i.e., materials in the image) at many narrow wavelength bands.…”

Section: S5mentioning

confidence: 99%

Seeing Cell Biology with the Eyes of Physics

Ventura¹

2017

NanoWorld J

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Rhythmic oscillatory patterns permeate the entire universe and sustain cellular dynamics at biological level. The intracellular environment is now regarded as a complex nanotopography embedding "intracrine" signals that encompass the intracellular action of regulatory molecules coupled with the newly discovered functions of the microtubuar network. Microtubuli, are far away from simply being a part of the cytoskeleton, since they produce both nanomechanical motions and display electromagnetic resonance modes that may turn local events into non-local, long-ranging paths. The possibility that microtubuli form a bioelectronic circuit prompts rethinking of biomolecular recognition as a result of synchronization of the oscillatory patterns of proteins sustained and orchestrated by resonance modes brought about by the microtubular network itself. Here, we discuss these issues within the biomedical perspective of using physical energies to govern (stem) cell fate. We focus on the ability of specially conveyed electromagnetic fields to afford optimization of stem cell polarity and pluripotency, reversing stem cell aging and promoting a multilineage repertoire in human adult stem cells, as well as in human non-stem somatic cells. We discuss the use atomic force microscopy and hyperspectral imaging for deciphering the nanomotions generated by (stem) cells during their growth and differentiation. We highlight the potential for unraveling vibrational signatures that can be exploited to direct the differentiation and self-healing potential of tissue-resident stem cells in vivo. In conclusion, seeing stem cell biology with the eyes of Physics may help developing a Regenerative/Precision medicine afforded through the stimulation of the natural ability of tissues for self-healing, without the needs of stem cell transplantation.

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“…It can be further extended with special Raman detection schemes (e.g. line scanning-mode [21], modulation of the excitation wavelength [15], or dual-polarization [11]) and supplemented with additional detection techniques like fluorescence [25,31], phase contrast [25], dark-field [31] microscopy, and others [53]. Moreover, custom systems can be combined with advanced sample handling techniques, like microfluidics [2,3,14] or optical tweezers [1,24,25,57].…”

Section: Introductionmentioning

confidence: 99%

Design and first applications of a flexible Raman micro-spectroscopic system for biological imaging

Kiselev

Schie

Aškrabić

et al. 2016

BSI

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Abstract. Typical commercial Raman micro-spectroscopic systems do not offer much flexibility to the end user, thus limiting potential research applications. We present a design of a simple, highly flexible and portable confocal Raman microscope with a detailed list of parts. The system can perform spectral acquisition in different modes: single-point spectroscopy, hyperspectral point mapping or hyperspectral line mapping. Moreover, the microscope can be easily converted between inverted and upright configurations, which can be beneficial for specific situations. Fiber coupling enables to connect various lasers for excitation and spectrometer/CCD combinations for signal detection. The performance of the instrument is demonstrated via Raman spectroscopy at 785 nm excitation wavelength, single point mapping of pancreatic cancer cells placed onto a quartz substrate and line mapping of polystyrene beads.

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Optical hyperspectral imaging in microscopy and spectroscopy - a review of data acquisition

Cited by 161 publications

References 112 publications

Innovative Filter Solutions for Hyperspectral Imaging

Innovative Filter Solutions for Hyperspectral Imaging

Seeing Cell Biology with the Eyes of Physics

Design and first applications of a flexible Raman micro-spectroscopic system for biological imaging

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