ILK expression in human basal cell carcinoma correlates with epithelial–mesenchymal transition markers and tumour invasion

Papanikolaou, Sofia; Bravou, Vasiliki; Gyftopoulos, Kostis; Nakas, Dimitrios; Repanti, Maria; Papadaki, Helen

doi:10.1111/j.1365-2559.2010.03556.x

Cited by 41 publications

(37 citation statements)

References 46 publications

(138 reference statements)

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“…Cytoplasmic and nuclear expression, when observed, was evaluated separately. Immunoreactivity was scored on a scale of 0-3 according to intensity of staining and percentage of positive cells as previously described (18). Staining intensity was graded as 0 (negative), 1 (weak), 2 (moderate), and 3 (strong).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Focal Adhesion Proteins α- and β-Parvin are Overexpressed in Human Colorectal Cancer and Correlate with Tumor Progression

Bravou

Antonacopoulou

Papanikolaou

et al. 2015

Cancer Investigation

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This study aims to address the role of focal adhesion proteins α- and β-parvin in human colorectal carcinoma (CRC). Expression of α- and β-parvin was examined by immunohistochemistry and real-time RT-PCR in a series of human CRC. Parvins were overexpressed in CRC and their expression correlated significantly with tumor invasion, lymph node metastasis, and disease stage. A significant positive correlation of parvins protein expression with overexpression of integrin-linked kinase, p-AKT, and nuclear β-catenin, as well as with downregulation of E-cadherin was also observed. In conclusion, overexpression of α- and β-parvin seems to be implicated in human colorectal cancer progression.

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Section: Immunohistochemistrymentioning

confidence: 99%

Focal Adhesion Proteins α- and β-Parvin are Overexpressed in Human Colorectal Cancer and Correlate with Tumor Progression

Bravou

Antonacopoulou

Papanikolaou

et al. 2015

Cancer Investigation

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“…[13][14][15] Increased snail and decreased E-cadherin are involved in the progression of cancer and the shortening of survival. 12,16 In the present study, we assessed the snail-positive rate of NSCs and cancer cells that show glandular lumen in each section, and we found that snail-positive rate was significantly greater in NSCs than in cancer cells that show glandular lumen. In addition, the snail-positive rate in NSCs was greater than that in cancer cells that show glandular lumen in all patients (in all patients, [snailpositive rate in NSCs] ≧ [snail-positive rate in cancer cells that show glandular lumen]).…”

Section: Discussionmentioning

confidence: 83%

Neoplastic Spindle Cells Are an Independent Prognostic Factor in Pancreatic Cancer

Takahashi

Hisaka²,

Horiuchi³

et al. 2015

Pancreas

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“…Twistl may be a feasible biomarker for highly invasive and/or metastatic BCC. The expression levels of Snail, the other transcription factor promoting EMT, have been shown to correlate with the depth of tumour invasion in BCC (8). However, no significant relationship was found between Snail and down-regulation of E-cadherin (8), suggesting that another transcription factor is involved in the regulation of EMT in BCC.…”

Section: Discussionmentioning

confidence: 98%

“…The expression levels of Snail, the other transcription factor promoting EMT, have been shown to correlate with the depth of tumour invasion in BCC (8). However, no significant relationship was found between Snail and down-regulation of E-cadherin (8), suggesting that another transcription factor is involved in the regulation of EMT in BCC. Our finding is in accordance with the notion that Twistl is capable of promoting EMT, contributing to aggressive invasion and multiple organ métastases.…”

Section: Discussionmentioning

confidence: 98%

Twist1 as a Possible Biomarker for Metastatic Basal Cell Carcinoma

Majima¹,

Hirakawa²,

Kito³

et al. 2012

Acta Derm Venerol

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Basal cell carcinoma (BCC), a common tumour of epithelial origin, is locally invasive and has a low risk of metastasis, estimated to range from 0.028% to 0.1% (1). However, once BCC metastasizes to distant organs, the effective therapeutic options are limited, leading to poor patient outcome.Recent studies have shown that epithelial-mesenchymal transition (EMT) suppresses epithelial features, and induces mesenchymal traits to epithelial cells in several pathological conditions (2,3). Of note is that tumours of epithelial origin can express transcription factors Snail and Twistl, or the cell adhesion molecule N-cadherin as a mesenchymal marker. We have previously shown that N-cadherin is up-regulated in invasive tumours, but not in carcinoma in situ of extramammary Paget's disease, and that EMT is associated with distant organ metastasis, leading to poor patient survival (4,5). However, it remains unclear whether EMT plays a key role ir the development of highly invasive phenotype and potential metastasis in BCC. We report here the first case of morphoeic and metastatic BCC showing the induction of Twistl and the epithelial-to-mesenchymal conversion of cadherins in association with multiple organ métastases. CASE REPORTA 51-year-old Japanese man presented with a 3-cm ulcerated nodule with peripheral focal pigmentation on the upper back ( Fig. la). A biopsy specimen disclosed the proliferation and invasion of epithelial strands with mesenchymal transition and rich stroma, indicating a diagnosis of morphoeic BCC (Fig. lb). The patient underwent a tumour resection, and the defect was reconstructed with the trapezius musculocutaneous flap. After 4 years of follow-up, a subcutaneous mass appeared in the patient's supraclavicular fossa. Under the histological diagnosis of metastatic BCC, the tumour was surgically resected with subsequent reconstruction using the pectoral major musculo-cutaneous nap, followed by a total of 60 Gy radiation to the local region. However, multiple foci of tumour metastasis were found in the left levator scapulae muscle and the pleura ofthe left lung by magnetic resonance imaging. The patient underwent systemic chemotherapy with a combination of cisplatin and doxorubicin. However, the therapeutic effect was minimal, and at the time of writing, the tumours were disseminated in the lung. Fig. 1. Clinical, roentgenological, and pathological features in metastatic basal cell carcinoma (BCC). (a) Clinical presentation of 3-cm ulcerated nodule on the upper back with peripheral, focal pigmentation, (b) Haematoxylin & eosin stains (x 400) ofthe cutaneous primary site show foci of basaloid tumour ceils on the superiorly and infiltrative tumour cells within the stroma. (c) Prominent Twistl expression was revealed in the nuclei of tumour cells (arrowheads) at the invasive front in the index case, (d) No Twist 1 expression was found in control representative nodular BCC by immunohistochemistry. Scale bars= 100 um (b); 30 \irri (c, d).To clarify the potential role of EMT in metastatic BCC, we investigated...

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ILK expression in human basal cell carcinoma correlates with epithelial–mesenchymal transition markers and tumour invasion

Abstract: ILK overexpression in BCC is implicated in tumour progression probably through the induction of an EMT-related molecular profile. Nuclear localization of E-cadherin in BCC is also associated with aggressive tumour features.

Cited by 41 publications

References 46 publications

Focal Adhesion Proteins α- and β-Parvin are Overexpressed in Human Colorectal Cancer and Correlate with Tumor Progression

Focal Adhesion Proteins α- and β-Parvin are Overexpressed in Human Colorectal Cancer and Correlate with Tumor Progression

Neoplastic Spindle Cells Are an Independent Prognostic Factor in Pancreatic Cancer

Twist1 as a Possible Biomarker for Metastatic Basal Cell Carcinoma

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