Focal Adhesion Proteins α- and β-Parvin are Overexpressed in Human Colorectal Cancer and Correlate with Tumor Progression

Bravou, Vasiliki; Antonacopoulou, Anna G.; Papanikolaou, Sofia; Nikou, Sofia; Lilis, Ioannis; Giannopoulou, Efstathia; Kalofonos, Haralabos P.

doi:10.3109/07357907.2015.1047508

Cited by 19 publications

(22 citation statements)

References 28 publications

(58 reference statements)

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“…5D) was overexpressed in CRC including COAD and READ (Fig. 5E), which supported the previous study that reported overexpression of PARVB correlated significantly with lymph node metastasis and tumor invasion (Bravou et al, 2015). Bifidobacterium dentium , Parvimonas micra and Porphyromonas asaccharolytica are all bacteria found in the human oral cavity that are normally in low abundance in the colon.…”

Section: Resultssupporting

confidence: 90%

M-GWAS for the gut microbiome in Chinese adults illuminates on complex diseases

Liu

Tang

Huang

et al. 2019

Preprint

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SummaryThe gut microbiome has been established as a key environmental factor to health. Genetic influences on the gut microbiome have been reported, yet, doubts remain as to the significance of genetic associations. Here, we provide shotgun data for whole genome and whole metagenome from a Chinese cohort, identifying no less than 20% genetic contribution to the gut microbiota. Using common variants-, rare variants- and copy number variations (CNVs)-based association analyses, we identified abundant signals associated with the gut microbiome especially in metabolic, neurological and immunological functions. The controversial concept of enterotypes may have a genetic attribute, with the top 2 loci explaining 11% of the Prevotella-Bacteroides variances. Stratification according to gender led to the identification of differential associations in males and females. Genetically encoded responses to ectopic presence of oral bacteria in the gut appear to be a common theme in a number of diseases investigated by MWAS (Metagenome-wide association studies). Our two-stage M-GWAS (Microbiome genome-wide association studies) on a total of 1295 individuals unequivocally illustrates that neither microbiome nor GWAS studies could overlook one another in our quest for a better understanding of human health and diseases.HighlightsM-GWAS using high-depth whole genome identifies contributions from rare variants and CNVs.Gut microbial modules such as butyrate, amino acids, mucin degradation show genetic associations.Gender differential M-GWAS underscores differences in metabolic and psychological predispositions.Some of the MWAS markers for colorectal cancer and cardiometabolic diseases show genetic associations.

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Section: Resultssupporting

confidence: 90%

M-GWAS for the gut microbiome in Chinese adults illuminates on complex diseases

Liu

Tang

Huang

et al. 2019

Preprint

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“…From TCGA database, we further discovered a trend towards worse DFS in PCa patients (Gleason score ≥7) with higher mRNA expression of PARVB . Previous studies reported conflicting outcomes, as PARVB was downregulated in breast cancer ( 26 ) and urothelial cell carcinoma ( 27 ), while overexpressed and correlated with tumor progression in colorectal cancer ( 12 ) and tongue squamous cell carcinoma ( 28 ), which implied an organ-specific expression or function of β-parvin.…”

Section: Discussionmentioning

confidence: 99%

“…Evaluation of sections were accomplished by two pathologists at our institute. Immunoreactivity score (IRS) category, determined by the multiplication of score of staining intensity and score of percentage of positive cells, was applied to assess the immunohistochemistry (IHC) sections as previously described ( 12 ). In specific, intensity of staining was classified as 0 (negative), 1 (weak), 2 (moderate), and 3 (strong), and percentage of positive cells was classified as 0 (<1%), 1 (1–25%), 2 (26–50%), 3 (51–75%), and 4 (76–100%).…”

Section: Methodsmentioning

confidence: 99%

Differential expressions of integrin‑linked kinase, β‑parvin and cofilin 1 in high‑fat diet induced prostate cancer progression in a transgenic mouse model

Jiang

et al. 2018

Oncol Lett

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High-fat diet induced obesity was associated with more aggressive prostate cancer. Recent research has demonstrated that integrin-linked kinase (ILK), β-parvin and downstream cofilin 1 jointly affected cancer progression. Meanwhile, these proteins were also involved in energy metabolism. Therefore, the present study was conducted to investigate the potential function of ILK, β-parvin and cofilin 1 in the high-fat diet-induced progression of prostate cancer. Transgenic mice with prostate cancer were employed, fed with different diets and sacrificed at 20 and 28 weeks. Tumor differentiation, extracapsular extension and metastasis were compared between the groups. Expression levels of ILK, β-parvin and cofilin 1 in prostate were evaluated by immunohistochemical analysis and determined by an immunoreactivity score. Public databases were applied for analysis and validation. It was detected that high-fat diet feeding promoted cancer progression in transgenic mice with prostate cancer, with increased expressions of β-parvin (P=0.038) and cofilin 1 (P=0.018). Higher expressions of ILK, β-parvin and cofilin 1 were also associated with poorer cancer differentiation. Additionally, higher mRNA levels of CFL1 were correlated with a worse disease-free survival in patients of certain subgroups from The Cancer Genome Atlas database. Further studies were warranted in discussing the potential roles of ILK, β-parvin and cofilin 1 in high-fat diet feeding induced progression of prostate cancer.

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“…The role of PARVB in tumor progression remains controversial. Some studies reported that PARVB was down-regulated in breast cancer and could inhibit tumorigenicity [23,24], while others argued that PARVB was overexpressed in colorectal carcinoma and seemed to be implicated in cancer progression [25]. Still others reported that it also increased the cell migration capability in tongue squamous cell carcinoma [15].…”

Section: Discussionmentioning

confidence: 99%

HIF-1α and HIF-2α Mediated PARVB Expression Promotes Tumor Growth and Metastasis in Malignantmelanoma

Wang

et al. 2020

Preprint

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BackgroundMalignant melanoma is the leading cause of skin cancer-related death. The role of PARVB in malignant melanoma remains unclear. Hypoxia is a hallmark of solid tumors including melanoma. But the regulation role of hypoxia in PARVB expression has not been reported.MethodsHuman malignant melanoma tissues, cell lines and their controls were collected. IHC staining, qRT-PCR and Western blot were performed to reveal the differential PARVB expression. The role of PARVB in tumor growth and metastasis of malignant melanoma was evaluated in vitro and in vivo. The regulation role and mechanism of hypoxia and HIFs in PARVB expression was validated by qRT-PCR, Western blot, ChIP-PCR and Luciferase reporter assays.ResultsPARVB was upregulated in malignant melanoma and correlated with patient survival. OverexpressionofPARVB promoted tumor growth and metastasis of malignant melanoma. Furthermore, hypoxia induced HIF-1α and HIF-2α expression activated PARVB transcription and expression through binding to the specific hypoxia-responsive element (HRE) in the promoter region of PARVB.ConclusionsIn malignant melanoma, Hypoxia induced HIF-1α and HIF-2α expression could directly activate PARVB expression, which further promoted tumor growth and metastasis, inducing poor prognosis. These results indicated that PARVB might be a potential therapeutic target for malignant melanoma.

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Focal Adhesion Proteins α- and β-Parvin are Overexpressed in Human Colorectal Cancer and Correlate with Tumor Progression

Cited by 19 publications

References 28 publications

M-GWAS for the gut microbiome in Chinese adults illuminates on complex diseases

M-GWAS for the gut microbiome in Chinese adults illuminates on complex diseases

Differential expressions of integrin‑linked kinase, β‑parvin and cofilin 1 in high‑fat diet induced prostate cancer progression in a transgenic mouse model

HIF-1α and HIF-2α Mediated PARVB Expression Promotes Tumor Growth and Metastasis in Malignantmelanoma

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