IL1 cluster gene polymorphisms in macedonian patients with chronic periodontitis

Abstract

Several studies have investigated the genetic polymorphisms for cytokines as potential genetic markers for periodontitis. The aim of this study was to determine the prevalence of IL1 cluster genes polymorphisms and their association with chronic periodontitis in the Macedonian population. The group of 114 unrelated Macedonian subjects with chronic periodontitis and 301 periodontitis-free Macedonian subjects were studied. DNA was isolated from peripheral blood leukocytes by phenol-chloroform extraction method. Cytokine genotyping was performed by PCR-SSP. The population genetics analysis package (PyPop) was used for analysis of the cytokine data for this report. Crude odds ratio (OR) was calculated as estimates of the relative risk with 95 % confi dence interval (CI). Genotype frequency of IL1B -511/C:T was signifi cantly higher in patients with periodontitis than in controls (OR=2.11, 95 % CI=1.35-3.32, p=0.001). IL1 cluster gene haplotype frequencies of TTTCT and TCTTT were associated with higher risk for periodontitis (OR=5.06, 95 % CI=1. p<0.0014 and OR=8.35, p<0.002, respectively). No signifi cant association of IL1 composite genotype (IL1 -889A:IL1B +3962) with periodontitis in Macedonians was found. The latter association was found to be signifi cant in genotype IL1B -511/C:T, haplotype TTTCT, and haplotype TCTTT, but without signifi cant association in IL1 composite genotype (Tab. 5, Ref. 43). Full Text in PDF www.elis.sk.

Meta‐Analysis of Association Between Interleukin‐1β C‐511T Polymorphism and Chronic Periodontitis Susceptibility

“…The literature search yielded 514 publications, and 19 case‐control studies 9,18‐35 involving 2,173 patients with CP and 3,900 healthy controls were included. Figure 1 shows the study selection process.…”

“…Of the 19 included studies, 12 were conducted in Asian participants, 18,19,22,26,28‐35 five in white participants, 9,20,21,23,27 and two in Brazilian (mixed) participants 24,25 . Controls of four studies 24,28,31,33 were out of HWE.…”

“…Controls of four studies 24,28,31,33 were out of HWE. Nine studies did not report the participants’ smoking statuses, 9,18,19,25,26,31,33‐35 six only included non‐smokers, 22,24,27‐29,32 and the remaining four studies 20,21,23,30 included participants of mixed smoking statuses. Table 1 shows the main characteristics of included studies.…”

Identification of Periopathogenes from Dental Plaque in Periodontal Patients with PCR Technique and Their Association with Composite Interleukin-1 Genotype

“…This finding is in favor of our research where significant association between the presence of composite genotype in the participants in the test group wasn't found on the other side composite genotype was registered at 8 of the patients with periodontitis in the control group. Also we didn't found significant association between the presence of the composite genotype and any of the tested periopathogen microorganism that only additionally confirms the inability to determine the association between the composite genotype and the microorganisms that cause periodontitis [39].…”

Quality of methods and reporting in association studies of chronic periodontitis and IL1A−889 and IL1B+3953/4 SNPs: A systematic review

“…Thirty‐eight studies made some efforts to control the effects of smoking habits. Twenty‐two studies excluded smokers, 10 articles used regression analysis, 1 adjusted the analysis of variance for smoking, 3 papers performed a stratified analysis, and the 2 remaining studies did not observe any statistically significant differences between cases and controls. Smoking habits were self‐reported by means of questionnaires.…”