IL‑1β promotes osteogenic differentiation of mouse bone marrow mesenchymal stem cells via the BMP/Smad pathway within a certain concentration range

Wang, Hao; Ni, Zhihao; Yang, Jiazhao; Li, Meng; Liu, Lei; Pan, Xuejie; Xu, Lei; Wang, Xujin; Fang, Shiyuan

doi:10.3892/etm.2020.9065

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…Wang et al suggested that, within a certain concentration range, interleukin (IL)-1β promotes the differentiation and osteogenic function of mouse bone marrow MSCs (MBMMSCs) through the bone morphogenetic protein/Smad (BMP/Smad) signaling pathway [ 71 ]. MBMMSCs proliferation in the presence of IL-1β was analyzed utilizing a Cell-Counting Kit-8 assay, and the effect of IL-1β on MBMMSC apoptosis was studied via flow cytometry.…”

Section: Factors That Induce Bone Formationmentioning

confidence: 99%

“…Othman et al identified two protein networks potentially involved in the osteoinduction process, one consisting of collagen fragments and collagen-related enzymes and another consisting of endopeptidase inhibitors and regulatory proteins. The authors' results show that protein profiling is a useful tool for understanding the interactions between a biomaterial and a biological system, which could help design and develop better biomaterials for use in bone regenerative therapies [60] [71]. MBMMSCs proliferation in the presence of IL-1β was analyzed utilizing a Cell-Counting Kit-8 assay, and the effect of IL-1β on MBMMSC apoptosis was studied via flow cytometry.…”

Section: Two Protein Network Are Potentially Implicated In Osteoindumentioning

confidence: 99%

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A Review of Recent Developments in the Molecular Mechanisms of Bone Healing

Rodríguez-Merchán

2021

IJMS

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Between 5 and 10 percent of fractures do not heal, a condition known as nonunion. In clinical practice, stable fracture fixation associated with autologous iliac crest bone graft placement is the gold standard for treatment. However, some recalcitrant nonunions do not resolve satisfactorily with this technique. For these cases, biological alternatives are sought based on the molecular mechanisms of bone healing, whose most recent findings are reviewed in this article. The pro-osteogenic efficacy of morin (a pale yellow crystalline flavonoid pigment found in old fustic and osage orange trees) has recently been reported, and the combined use of bone morphogenetic protein-9 (BMP9) and leptin might improve fracture healing. Inhibition with methyl-piperidino-pyrazole of estrogen receptor alpha signaling delays bone regeneration. Smoking causes a chondrogenic disorder, aberrant activity of the skeleton’s stem and progenitor cells, and an intense initial inflammatory response. Smoking cessation 4 weeks before surgery is therefore highly recommended. The delay in fracture consolidation in diabetic animals is related to BMP6 deficiency (35 kDa). The combination of bioceramics and expanded autologous human mesenchymal stem cells from bone marrow is a new and encouraging alternative for treating recalcitrant nonunions.

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Section: Factors That Induce Bone Formationmentioning

confidence: 99%

Section: Two Protein Network Are Potentially Implicated In Osteoindumentioning

confidence: 99%

A Review of Recent Developments in the Molecular Mechanisms of Bone Healing

Rodríguez-Merchán

2021

IJMS

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“…The role of various inflammatory mediators in osteogenic differentiation of MSCs is controversial. It has been described that mediators such as IL-1β and TNF-α can exert an adverse effect on the differentiation of murine MSCs into osteoblasts [46], although both mediators at low concentrations can also promote osteogenic differentiation in vitro [47,48]. Additionally, gene expression of the pleiotropic growth factor TGF-β1 was detected in all groups, and this can exert anti-inflammatory and pro-or anti-osteogenic effects depending on the context [49].…”

Section: Discussionmentioning

confidence: 91%

Pretreatment of Mesenchymal Stem Cells with Electrical Stimulation as a Strategy to Improve Bone Tissue Engineering Outcomes

Bianconi,

Oliveira,

Klein

et al. 2023

Cells

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Electrical stimulation (EStim), whether used alone or in combination with bone tissue engineering (BTE) approaches, has been shown to promote bone healing. In our previous in vitro studies, mesenchymal stem cells (MSCs) were exposed to EStim and a sustained, long-lasting increase in osteogenic activity was observed. Based on these findings, we hypothesized that pretreating MSC with EStim, in 2D or 3D cultures, before using them to treat large bone defects would improve BTE treatments. Critical size femur defects were created in 120 Sprague–Dawley rats and treated with scaffold granules seeded with MSCs that were pre-exposed or not (control group) to EStim 1 h/day for 7 days in 2D (MSCs alone) or 3D culture (MSCs + scaffolds). Bone healing was assessed at 1, 4, and 8 weeks post-surgery. In all groups, the percentage of new bone increased, while fibrous tissue and CD68+ cell count decreased over time. However, these and other healing features, like mineral density, bending stiffness, the amount of new bone and cartilage, and the gene expression of osteogenic markers, did not significantly differ between groups. Based on these findings, it appears that the bone healing environment could counteract the long-term, pro-osteogenic effects of EStim seen in our in vitro studies. Thus, EStim seems to be more effective when administered directly and continuously at the defect site during bone healing, as indicated by our previous studies.

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“…These results are consistent with the ARS and show that the BMMSCs' expression and gene expression imply enhanced mineralization and proliferation. Wang et al found that IL-1β enhances osteogenic gene expression and differentiation in BMMSCs without triggering early mortality during bone healing 53 . This might be because the sterile in ammatory response is active at all stages but loses its osteogenic induction effect at high dosages.…”

Section: Discussionmentioning

confidence: 99%

Modulating proliferative and osteogenic potentials of mesenchymal stem cells via a novel formulation of MTA as a root perforation repair material

Elgendi,

Hassanien,

Nagy

et al. 2024

Preprint

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Root perforations may cause periodontitis, resulting in alveolar bone loss. This study compared the zirconia containing MTA Matreva® (MAT group) to traditional MTA Angelus® (ANG group) as root perforation repair materials regarding the cytotoxicity and the osteogenic potential. Bone marrow mesenchymal stem cells (BM-MSCs) were isolated from two albino rats and cultured. Cytotoxicity of the tested materials was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay at three-time intervals; 3, 7 and 14 days. The tested materials were used to direct the osteogenic differentiation of BM-MSCs into osteoblasts. Evaluation of osteogenic effect was performed through assessments of calcium deposition by Alizarin red staining (ARS), expression of receptor activator of nuclear factor-kappa B (NF-κB) ligand (RANKL) protein in BM-MSCs seeded on the tested materials for 14 days and osteogenic potential gene markers expression in BM-MSCs after culture on the tested materials for 14 days. The assessed genes included; tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), and interleukin 1-β (IL-1β). All data were statistically analyzed. There were no significant differences between ANG and MAT groups in terms of cytotoxicity and osteogenic potential (P>0.05). The highest level of cell proliferation occurred in MAT groups, followed by ANG group, compared to negative controls (NC). The MAT group showed the highest levels of Average % calcific area fraction (90%) followed by that of ANG group (68%) as compared to NC (48%). The BM-MSCs in the MAT group exhibited significantly highest level of RANKL protein expression, followed by that of ANG group. The ANG group revealed significantly greater TNF-α and IL-1β levels than those in NC. The MAT group had significantly greater value of TGF-β than that of NC. Zirconia containing MTA (MTA Matreva) has comparable cytotoxicity and osteogenic potential to traditional MTA (MTA Angelus), which is regarded a dependable material for root perforation healing.

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IL‑1β promotes osteogenic differentiation of mouse bone marrow mesenchymal stem cells via the BMP/Smad pathway within a certain concentration range

Cited by 8 publications

References 41 publications

A Review of Recent Developments in the Molecular Mechanisms of Bone Healing

A Review of Recent Developments in the Molecular Mechanisms of Bone Healing

Pretreatment of Mesenchymal Stem Cells with Electrical Stimulation as a Strategy to Improve Bone Tissue Engineering Outcomes

Modulating proliferative and osteogenic potentials of mesenchymal stem cells via a novel formulation of MTA as a root perforation repair material

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