IL-1β Genetic Polimorphism in Menopause Women as Periodontal Disease Risk Factor

Abstract

Periodontal disease is a disease with multifactorial etiology that can occur in postmenopausal women. Objective: This research aims to know the description of the genetic polymorphism of IL-1β in postmenopausal women in Indonesia as a risk factor for periodontal disease. Methods: This study is a descriptive study on 55 biological stored amples. Sample's extracted DNA was analysed for polymorphisms IL -1β +3954 using PCR-RFLP method with Taq α 1 restriction enzyme digests then the result was electroforized. Research results were analyzed using chi square test. Results: The genotype CC frequency was 85.7%, CT 14.3%, and TT was absent in normal people. CC 86.5%, CT 8.3%, and TT did not exist in people who have periodontal disease. Conclusion: Genetic polymorphisms IL-1β in Indonesia's postmenopausal women is not a risk factor for periodontal disease ABSTRAK Polimorfisme genetik IL-1β pada wanita menopause sebagai faktor risiko penyakit periodontal. Penyakit periodontal merupakan penyakit oral dengan etiologi yang multifaktorial serta dapat terjadi pada wanita postmenopause. Tujuan: Penelitian ini bertujuan untuk mengetahui gambaran polimorfisme genetik IL-1β pada populasi wanita postmenopause Indonesia sebagai faktor risiko penyakit periodontal. Metode: Penelitian ini merupakan studi deskriptif menggunakan sampel biologi tersimpan sebanyak 55 buah. Hasil ekstraksi DNA, dilakukan pemeriksaan polimorfisme IL-1β +3954 menggunakan metode PCR-RFLP dengan digest enzim restriksi Taq α 1 kemudian hasilnya dielektroforesis. Hasil penelitian dianalisis menggunakan tes chi-square. Hasil: Gambaran frekuensi genotipe adalah CC 85,7%, CT 14,3%, dan TT tidak ada pada orang yang normal. CC 91,7%, CT 8,3%, and TT tidak ada pada orang yang memiliki penyakit periodontal. Simpulan: Polimorfisme genetik IL-1β pada wanita pascamenopause Indonesia bukan merupakan faktor risiko penyakit periodontal

Potency of stored DNA derived from buccal mucosal epithelial cells for genotype analysis of interleukin 4 -590 C/T

“…Research using stored DNA has also been carried out to analyze genetic variation in IL-1 beta in menopausal women. 8 Genotype analysis with PCR RFLP method was developed in 1987 and is a quite complex laboratory experiment.9 Enzym restriction must be sufficient in quantity and quality to obtain optimum results. This study on the IL-4 gene, especially in promoter at spot 590 showed some difficulties in digesting the samples.…”

Perbedaan Indeks Kebutuhan Perawatan Periodontal (Cpitn) Anak Normal Dan Anak Tunarungu

“…Koloni bakteri jika dibiarkan dan melekat pada permukaan gigi atau di bawah margin gingival akan menyebabkan gingivitis dan bila berlanjut bisa menyebabkan periodontitis. 1 Penyakit periodontal banyak ditemukan pada pasien dengan oral hygiene yang buruk. Penyakit periodontal juga dapat menjadi manifestasi oral dari beberapa penyakit sistemik.…”

The Difference Number of Bacterial Colonies between Smokers and Non-smokers Gingivitis Patients in Periodontics Department at USU Dental Hospital

“…Periodontal disease is a chronic inflammatory disease caused by bacterial invasion in the periodontal tissues, which involves the gingiva, periodontal ligament, alveolar bone and cementum [3,4]. The main cause of periodontal disease is the colonization of microorganisms that firmly attached to the tooth surface.…”

“…The main cause of periodontal disease is the colonization of microorganisms that firmly attached to the tooth surface. The bacterial colonies form the plaque that causes gingivitis [3].…”