Identifikasi Dan Uji Daya Hambat Isolat Bakteri Endofit Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Terhadap Bakteri Patogen

Sepriana, Citra; Sumiati, Eti; Jekti, Dwi Soelistya Dyah; Zulkifli, Lalu

doi:10.29303/jppipa.v6i1.340

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…These bacteria tend to be present as endophytes in the three pulp samples used, namely Aren, Coconut, and Sago, as normal flora. This study was in line with other studies, which found that one of the endophytic bacteria in rambutan fruit flesh was the genus that belongs to Staphylococcus [34].…”

Section: Figure 1 the Inhibitory Activity Of Isolate Endophytes Of E Coli Bacteriasupporting

confidence: 93%

Characterization and Antibacterial Activity of Endophytic Bacteria from Flesh Fruit of the Arecaceae Family Against Antibiotic Resistant Bacteria Escherichia coli

Harahap

2021

Biotropika

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Bacteria can symbiotic mutualism and synergize with plants in the tissue and do not cause harm to plants. On the other hand, bacteria resistance provides an opportunity to search for antibacterials from natural material without threatening plant biodiversity. The purpose of this study was to characterize and test the antibacterial activity of endophytic bacteria from the fruit of the Arecaceae family against antibiotic-resistant bacteria Escherichia coli. The method used was direct culture on the growth of media in the laboratory by first sterilizing the surface of the flesh of the samples, such as Aren (Arenga pinnata), Coconut (Cocos nucifera), and Sago (Metroxylon sagu). The pure isolates obtained were characterized according to Bergeys Manual for Identification by performing Gram stain, catalase, and coagulase test. The results obtained were five isolates with codes A1, A2, K, S1, and S2 with variation in morphology colonies. Gram staining, catalase, and coagulase verification of the five isolates were groups of Grampositive cocci, catalase-positive, and coagulase-negative bacteria. Based on tests carried out on the characters of the endophytic bacteria, it was obtained a close relationship with the genus Staphylococcus. The results of the inhibitory activity test showed that Staphylococcus sp. K with the highest criteria of 43.26 mm and Staphylococcus sp. A1 with the lowest inhibition criteria with a value of 24.73 mm. Both are categorized as very strong inhibition criteria.

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Section: Figure 1 the Inhibitory Activity Of Isolate Endophytes Of E Coli Bacteriasupporting

confidence: 93%

Characterization and Antibacterial Activity of Endophytic Bacteria from Flesh Fruit of the Arecaceae Family Against Antibiotic Resistant Bacteria Escherichia coli

Harahap

2021

Biotropika

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“…Kondisi awal (pre) PCR diatur pada suhu 94°C selama 5 menit, kemudian diikuti dengan 35 siklus PCR yang terdiri dari denaturasi pada suhu 94°C selama 30 detik, annealing primer pada suhu 55°C selama 30 detik dan ekstensi pada suhu 72°C selama 45 detik. Setelah 35 siklus dilalui, selanjutnya dilakukan post PCR pada suhu 72°C selama 5 menit dan disimpan pada suhu 20°C (Sepriana et al, 2020)…”

Section: Identifikasi Molekuler Isolatunclassified

DNA Barcoding of Endophytic Bacteria from The Stems of Tissue Piper crocatum and Activity Anti-Bacterial Properties

Amrullah

Zulkifli²

2022

JBT

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Secondary metabolites from herbal are commonly used to treat disease due to infection from pathogenic bacteria, endophytic bacteria is shortcut to collect secondary metabolites more effective than extracting direct from the plant. This research aims to obtain endophytic bacterial isolates from the stems of the red betel plant which can be used as an inhibitor for the growth of pathogenic bacteria. The method of this research is exploratory descriptive experiment based research. The stages of this research consist of few techniques which are isolation of endophytic, antibacterial examination, morphological, physiological, and biochemical observation, DNA bacterial extraction, gel electrophoresis. To analyse DNA Barcoding using 16S rRNA gen, BLAST and MEGA 10. The results of the antibacterial activity of the isolates showed by clear zone formation against pathogenic bacteria such as E. coli, K. pneumoniae and S. aureus. Endophytic bacterial that successfully isolated are labelled (BSM1, BSM2, BSM3 and BSM4). Based on of the Gel electrophoresis shows that four endophytic bacterial isolates are positive by appearance of bands in 1324bp. Based on the results of the phylogenetic tree it can be concluded that BSM1 is closely related to Bacillus cereus JL with a genetic distance of 0.007, while the genetic distance of BSM2 was 0.012, BSM3 0.006 and BSM4 0.003, all three were closely related to Bacillus pumilus AUEC29.

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“…Kondisi awal (pre) PCR diatur pada suhu 94 o C selama 5 menit, selanjutnya diikuti dengan 35 siklus PCR yang terdiri dari denaturasi pada suhu 94 o C selama 30 detik, annealing primer pada suhu 55 o C selama 30 detik dan ekstensi pada suhu 72 o C selama 45 detik. Setelah 35 siklus terlampaui, dilakukan post PCR pada suhu 72 o C selama 5 menit dan disimpan pada suhu 20 o C (Sepriana et al, 2020…”

Section: Identifikasi Molekuler Isolat Bakteri Pelarut Fosfatunclassified

Screening and Molecular Identification of Phosphate-Solubilizing Rhizobacteria from Mangrove Ecosystem of the Lombok Island

Zulkifli

Sedijani

Rasmi

et al. 2020

JBT

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Phosphate solubilizing rhizobacteria can be used as a component in biofertilizer formulations to increase local and national food production without causing adverse risks to the environment compared to the use of chemical fertilizers. In this regard, screening and identification of phosphate solubilizing bacteria from the rhizosphere of several mangrove species that grow on the coast of Lombok Island has been carried out. The method of isolation used is the Pikovskaya method. Screening and identification activities have obtained 5 isolates of mangrove rhizosphere bacteria (BRM) which are indicated by their ability to form clear zones on Pikovskaya media, namely isolates BRM1 and BRM4 (isolated from the rhizosphere of Avicennia marina), BRM2 and BRM3 (isolated from the rhizosphere of Rhizophora apicullata), BRM5 (isolated from the rhizosphere of R. stylosa). All rhizobacteria isolates were identified as Gram-positive bacteria. Molecular analysis based on the comparison of 16S rRNA gene sequences of isolates with the genome database at GenBank (NCBI) using Mega 10 software, showed that all BRM isolates occupied the same cluster as bacterial species from the Genus Paenibacillus in the dendrogram of the phylogenetic tree, namely Paenibacillus sp. JWLB1 strain, Paenibacillus sp. Strain NO13, P. cineris strain cu1-7, P. favisporus strain CHP14, with genetic distance ranging from 1.3 to 1.4. Many species of the Genus Paenibacillus are currently known to play an important roles as plant growth-promoting bacteria. The BRM isolates obtained in this study can be further developed as a biofertilizer component (inoculant) in saline and dryland agriculture.

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Identifikasi Dan Uji Daya Hambat Isolat Bakteri Endofit Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Terhadap Bakteri Patogen

Cited by 4 publications

References 15 publications

Characterization and Antibacterial Activity of Endophytic Bacteria from Flesh Fruit of the Arecaceae Family Against Antibiotic Resistant Bacteria Escherichia coli

Characterization and Antibacterial Activity of Endophytic Bacteria from Flesh Fruit of the Arecaceae Family Against Antibiotic Resistant Bacteria Escherichia coli

DNA Barcoding of Endophytic Bacteria from The Stems of Tissue Piper crocatum and Activity Anti-Bacterial Properties

Screening and Molecular Identification of Phosphate-Solubilizing Rhizobacteria from Mangrove Ecosystem of the Lombok Island

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